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Product Details
PA1-37625
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide from C-terminus of human e-NOS
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.6, with 1% BSA
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2153171
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is placenta, lung or ovarian carcinoma.
Target Information
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cNOS; Constitutive NOS; e-NOS; EC-NOS; Endothelial NOS; Nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; Nitric oxide synthase, endothelial; NOS type III; NOSIII; OTTHUMP00000213183
Gene Aliases: ECNOS; eNOS; NOS3
UniProt ID: (Human) P29474
Entrez Gene ID: (Human) 4846
actin monomer binding
nitric-oxide synthase activity
iron ion binding
protein binding
calmodulin binding
FMN binding
heme binding
tetrahydrobiopterin binding
arginine binding
cadmium ion binding
flavin adenine dinucleotide binding
NADP binding
scaffold protein binding
oxidoreductase
protein modifying enzyme
angiogenesis
ovulation from ovarian follicle
in utero embryonic development
blood vessel remodeling
regulation of sodium ion transport
regulation of the force of heart contraction by chemical signal
regulation of systemic arterial blood pressure by endothelin
arginine catabolic process
nitric oxide biosynthetic process
mitochondrion organization
nitric oxide mediated signal transduction
regulation of blood pressure
negative regulation of cell proliferation
response to heat
negative regulation of platelet activation
negative regulation of muscle hyperplasia
smooth muscle hyperplasia
removal of superoxide radicals
lung development
positive regulation of guanylate cyclase activity
lipopolysaccharide-mediated signaling pathway
response to fluid shear stress
negative regulation of potassium ion transport
endothelial cell migration
cell redox homeostasis
positive regulation of angiogenesis
negative regulation of blood pressure
positive regulation of vasodilation
regulation of blood vessel size
regulation of nitric-oxide synthase activity
negative regulation of hydrolase activity
negative regulation of calcium ion transport
oxidation-reduction process
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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