Invitrogen

eNOS Polyclonal Antibody

9 Published Figures

28 References

View all (45) eNOS antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)
Published Figures (9)

eNOS Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

eNOS Antibody (PA3-031A) in ICC/IF

Immunofluorescent analysis of eNOS using eNOS Polyclonal Antibody (Product # PA3-031A) shows staining in A2058 Cells. eNOS (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing eNOS (Product # PA3-031A) at a dilution of 1:200 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552 for GAR, Product # 35... View More

eNOS Antibody in Immunohistochemistry (Paraffin) (IHC (P))

eNOS Antibody in Immunohistochemistry (IHC)

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Product Details

PA3-031A

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 16 publications 16 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:250

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:250

View 4 publications 4 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Dog, Human, Mouse, Rat

Published species

Bovine, Dog, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues P(599) Y N S S P R P E Q H K S Y K(613) C of bovine eNOS.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum, PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2251447

Product Specific Information

PA3-031A detects endothelial Nitric Oxide Synthase (eNOS) from canine, bovine, human, mouse and rat tissues. This antibody does not detect brain NOS (bNOS) or inducible NOS (iNOS).

PA3-031A has been successfully used in Western blot, immunohistochemistry, immunofluorescence, and immunocytochemistry procedures. By Western blot, this antibody detects an approximately 140 kDa band representing eNOS from canine cardiac extracts. Immunohistochemical staining of eNOS in mouse cardiac tissue with PA3-031 results in the staining of the endothelium.

The PA3-031A immunogen is a synthetic peptide corresponding to residues P(599) Y N S S P R P E Q H K S Y K(613) C of bovine eNOS.

Target Information

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Hepatoprotective effect of nicorandil against acetaminophen-induced oxidative stress and hepatotoxicity in mice via modulating NO synthesis.

Environmental science and pollution research international

El-Kashef DH,Sharawy MH

Published figure using eNOS polyclonal antibody (Product # PA3-031A) in Immunohistochemistry

Wed Feb 01 00:00:00 EST 2023

Skin wound healing effects of (+)-syringaresinol from ginseng berry.

Journal of ginseng research

Hwang JH,Kang Y,Park HJ,Kim S,Lee SH,Kim H,Nam SJ,Lim KM

Published figure using eNOS polyclonal antibody (Product # PA3-031A) in Immunohistochemistry

Fri Sep 01 00:00:00 EDT 2023

Chronic exposure to tramadol induces cardiac inflammation and endothelial dysfunction in mice.

Scientific reports

Bakr MH,Radwan E,Shaltout AS,Farrag AA,Mahmoud AR,Abd-Elhamid TH,Ali M

Published figure using eNOS polyclonal antibody (Product # PA3-031A) in Immunohistochemistry

Tue Sep 21 00:00:00 EDT 2021

Activation of the mechanosensitive Ca²⁺ channel TRPV4 induces endothelial barrier permeability via the disruption of mitochondrial bioenergetics.

Redox biology

Lu Q,Zemskov EA,Sun X,Wang H,Yegambaram M,Wu X,Garcia-Flores A,Song S,Tang H,Kangath A,Cabanillas GZ,Yuan JX,Wang T,Fineman JR,Black SM

PA3-031A was used in Immunohistochemistry-immunofluorescence to investigate the role of endothelial nitric oxide synthase uncoupling in the TRPV4 activation-associated barrier disruption.

Fri Jan 01 00:00:00 EST 2021

Activation of the mechanosensitive Ca²⁺ channel TRPV4 induces endothelial barrier permeability via the disruption of mitochondrial bioenergetics.

Redox biology

Lu Q,Zemskov EA,Sun X,Wang H,Yegambaram M,Wu X,Garcia-Flores A,Song S,Tang H,Kangath A,Cabanillas GZ,Yuan JX,Wang T,Fineman JR,Black SM

PA3-031A was used in Immunohistochemistry-immunofluorescence to investigate the role of endothelial nitric oxide synthase uncoupling in the TRPV4 activation-associated barrier disruption.

Fri Jan 01 00:00:00 EST 2021

ASS1 and ASL suppress growth in clear cell renal cell carcinoma via altered nitrogen metabolism.

Cancer & metabolism

Khare S,Kim LC,Lobel G,Doulias PT,Ischiropoulos H,Nissim I,Keith B,Simon MC

PA3-031A was used in Immunohistochemistry to elucidate the contributions of urea cycle enzymes, argininosuccinate synthase 1 (ASS1), and argininosuccinate lyase (ASL) towards ccRCC progression.

Fri Dec 03 00:00:00 EST 2021

Laminar flow substantially affects the morphology and functional phenotype of glomerular endothelial cells.

PloS one

't Hart DC,van der Vlag J,Nijenhuis T

PA3-031A was used in Immunocytochemistry-immunofluorescence to investigate the influence of laminar flow-induced shear stress on the glomerular endothelium.

Mon Oct 18 00:00:00 EDT 2021

Laminar flow substantially affects the morphology and functional phenotype of glomerular endothelial cells.

PloS one

't Hart DC,van der Vlag J,Nijenhuis T

PA3-031A was used in Immunocytochemistry-immunofluorescence to investigate the influence of laminar flow-induced shear stress on the glomerular endothelium.

Mon Oct 18 00:00:00 EDT 2021

PGC1α is required for the renoprotective effect of lncRNA Tug1 in vivo and links Tug1 with urea cycle metabolites.

Cell reports

Li L,Long J,Mise K,Galvan DL,Overbeek PA,Tan L,Kumar SV,Chan WK,Lorenzi PL,Chang BH,Danesh FR

PA3-031A was used in Flow cytometry/Cell sorting to generate a triple-mutant diabetic mouse model coupled with metabolomic profiling data to interrogate whether Tug1 interaction with peroxisome proliferator-activated receptor gamma coactivator 1α (PGC1α) is required for mitochondrial remodeling and progression of DN in vivo.

Tue Aug 10 00:00:00 EDT 2021

NOS cofactor tetrahydrobiopterin contributes to anesthetic preconditioning induced myocardial protection in the isolated ex vivo rat heart.

International journal of molecular medicine

Wang C,Qiao S,Hong L,Sun J,Che T,An J,Camara AKS

PA3-031A was used in Western Blot to observe the role of BH4 availability and the association of HSP90 with NOS3 in APC‚mediated cardioprotection against I/R injury.

Sat Feb 01 00:00:00 EST 2020

Cyclic compression increases F508 Del CFTR expression in ciliated human airway epithelium.

American journal of physiology. Lung cellular and molecular physiology

Marozkina N,Bosch J,Cotton C,Smith L,Seckler J,Zaman K,Rehman S,Periasamy A,Gaston H,Altawallbeh G,Davis M,Jones DR,Schilz R,Randell SH,Gaston B

PA3-031A was used in Immunocytochemistry-Immunofluorescence to study how proteins relevant to lung health are affected by cyclic increases in apical air pressure.

Thu Aug 01 00:00:00 EDT 2019

Overexpression of 11β-hydroxysteroid dehydrogenase 1 in visceral adipose tissue and underexpression of endothelial nitric oxide synthase in the adrenal cortex of dogs with hyperadrenocorticism.

Open veterinary journal

Miceli DD,Abiuso AMB,Vidal PN,Gallelli MF,Pignataro OP,Castillo VA

PA3-031A was used in Immunohistochemistry to evaluate 11Β-Hydroxysteroid dehydrogenase 1 and endothelial nitric oxide synthase expression in dogs with hyperadrenocorticism.

Wed Nov 20 00:00:00 EST 2019

Glutamine prevents oxidative stress in a model of portal hypertension.

World journal of gastroenterology

Zabot GP,Carvalhal GF,Marroni NP,Licks F,Hartmann RM,da Silva VD,Fillmann HS

Published figure using eNOS polyclonal antibody (Product # PA3-031A) in Immunohistochemistry

Fri Jul 07 00:00:00 EDT 2017

The elevation of intraocular pressure is associated with apoptosis and increased immunoreactivity for nitric oxide synthase in rat retina whereas the effectiveness of retina derived relaxing factor is unaffected.

Experimental eye research

Takır S,Gürel-Gürevin E,Toprak A,Demirci-Tansel C,Uydeş-Doğan BS

PA3-031A was used in immunohistochemistry - paraffin section to investigate the association with apoptosis and increased immunoreactivity for nitric oxide synthase in rat retina causing an elevation of intraocular pressure and how the retina derived rela

Fri Apr 01 00:00:00 EDT 2016

Ameliorative effects of pentoxifylline on NOS induced by diabetes in rat kidney.

Renal failure

Sönmez MF,Dündar M

PA3-031A was used in immunohistochemistry - paraffin section to analyze NOS induced by diabetes in rat kidney and ameliorative effects of pentoxifylline

Wed Jan 04 00:00:00 EST 2017

Nitric oxide synthase in diabetic rat testicular tissue and the effects of pentoxifylline therapy.

Systems biology in reproductive medicine

Sönmez MF,Kılıç E,Karabulut D,Çilenk K,Deligönül E,Dündar M

PA3-031A was used in immunohistochemistry and western blot to determine the effects of pentoxifylline therapy and nitric oxide synthase in diabetic rat testicular tissue

Wed Oct 19 00:00:00 EDT 2016

Therapeutic effects of pentoxifylline on diabetic heart tissue via NOS.

Anatolian journal of cardiology

Karabulut D,Ulusoy HB,Kaymak E,Sönmez MF

PA3-031A was used in Immunohistochemistry to find that diabetes causes serious damage in heart tissue via NOS, and pentoxifylline aided in improving nNOS and iNOS expression.

Sun May 01 00:00:00 EDT 2016

GSPE is superior to NAC in the prevention of contrast-induced nephropathy: might this superiority be related to caspase 1 and calpain 1?

Life sciences

Ulusoy S,Ozkan G,Mungan S,Orem A,Yulug E,Alkanat M,Yucesan FB

PA3-031A was used in immunohistochemistry to study the potential role of calpain-1 in the greater protective effect against contrast-induced nephropathy of grape seed proanthocyanidin extract as compared to N-acetyl cysteine

Sat May 17 00:00:00 EDT 2014

Perspective on rhabdomyolysis-induced acute kidney injury and new treatment options.

American journal of nephrology

Ulusoy S,Ozkan G,Alkanat M,Mungan S,Yuluğ E,Orem A

PA3-031A was used in immunohistochemistry to study the protective effects of a grape seed proanthocyanidin extract in a rat model of acute kidney injury induced by rhabdomyolysis

Mon Jul 21 00:00:00 EDT 2014

Immunohistochemical expression of nitric oxide synthase enzymes (iNOS, eNOS, nNOS) in the estrual and luteal phases of the sexual cycle in the cow oviduct.

Anatomia, histologia, embryologia

Özen A,Ergün E,Öztaş E,Ergün L,Özcan Z,Alabay B,Bayraktaroğlu AG,Kürüm A,Erdoğan E

PA3-031A was used in immunohistochemistry to study the bovine oviduct expression of iNOS, eNOS and nNOS across the sexual cycle

Tue Oct 01 00:00:00 EDT 2013

Caloric restriction increases internal iliac artery and penil nitric oxide synthase expression in rat: comparison of aged and adult rats.

Archivio italiano di urologia, andrologia : organo ufficiale [di] Societa italiana di ecografia urologica e nefrologica

Ozbek E,Simsek A,Ozbek M,Somay A

PA3-031A was used in immunohistochemistry to study the expression of eNOS and nNOS in the internal iliac artery and penis of rats subjected to caloric restriction

Thu Sep 26 00:00:00 EDT 2013

Sympathetic nervous system regulates bone marrow-derived cell egress through endothelial nitric oxide synthase activation: role in postischemic tissue remodeling.

Arteriosclerosis, thrombosis, and vascular biology

Récalde A,Richart A,Guérin C,Cochain C,Zouggari Y,Yin KH,Vilar J,Drouet I,Lévy B,Varoquaux O,Silvestre JS

PA3-031A was used in immunocytochemistry to investigate the mechanism of the regulation of bone marrow-derived cell egress by sympathetic nervous system

Thu Mar 01 00:00:00 EST 2012

Changes in renal vessels following the long-term administration of an angiotensin II receptor blocker in Zucker fatty rats.

Journal of the renin-angiotensin-aldosterone system : JRAAS

Nakanishi K,Nagai Y,Akimoto T,Kato H,Yanakieva-Georgieva N,Ishikawa Y,Yoshihara K,Ito K,Yamanaka N,Oite T

PA3-031A was used in immunohistochemistry to identify how long term exposure to angiotensin II receptor blockers changes renal vessels

Wed Jun 01 00:00:00 EDT 2011

The human placenta from heavy smokers: evaluation of vasoactive peptides by immunohistochemistry.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica

Clausen HV,Larsen LG,Jørgensen A,Bzorek M

PA3-031A was used in immunohistochemistry to investigate the expression of e-NOS and endothelin-1 in human placenta from heavy smokers and non-smokers

Mon Jan 01 00:00:00 EST 2007

Expression of nitric oxide synthase and effect of substrate manipulation of the nitric oxide pathway in mouse ovarian follicles.

Human reproduction (Oxford, England)

Mitchell LM,Kennedy CR,Hartshorne GM

PA3-031A was used in immunocytochemistry to investigate the expression and role of nitric oxide synthase in mouse ovarian follicles

Thu Jan 01 00:00:00 EST 2004

Warfarin reduces the incidence of osteonecrosis of the femoral head in spontaneously hypertensive rats.

Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association

Wada M,Kumagai K,Murata M,S-Yamashita Y,Shindo H

PA3-031A was used in immunohistochemistry to study the effect of warfarin on the osteonecrosis of femoral head in spontaneously hypertensive rats

Tue Dec 13 00:00:00 EST 2005

Decreased expression of myocardial eNOS and caveolin in dogs with hypertrophic cardiomyopathy.

American journal of physiology. Heart and circulatory physiology

Piech A,Massart PE,Dessy C,Feron O,Havaux X,Morel N,Vanoverschelde JL,Donckier J,Balligand JL

PA3-031A was used in western blot to investigate the changes of eNOS and caveolin-1 and -3 in their respective expression level in a dog model of moderate, nonfailing, hypertrophic cardiomyopathy

Tue Jan 01 00:00:00 EST 2002

GnRH agonist-suppressed expression of nitric oxide synthases and generation of peroxynitrite in adenomyosis.

Human reproduction (Oxford, England)

Kamada Y,Nakatsuka M,Asagiri K,Noguchi S,Habara T,Takata M,Kudo T

PA3-031A was used in immunohistochemistry to investigate the role of gonadotrophin-releasing hormone agonists during nitric oxide synthase expression and peroxynitrite generation in adenomyosis

Fri Dec 01 00:00:00 EST 2000

Experimental allergic encephalomyelitis in the rat is inhibited by aminoguanidine, an inhibitor of nitric oxide synthase.

Journal of neuroimmunology

Zhao W,Tilton RG,Corbett JA,McDaniel ML,Misko TP,Williamson JR,Cross AH,Hickey WF

PA3-031A was used in immunohistochemistry to investigate the role of nitric oxide production in the pathogenesis of rat experimental allergic encephalomyelitis

Thu Feb 01 00:00:00 EST 1996

Bioinformatics

Protein Aliases: cNOS; Constitutive NOS; e-NOS; EC-NOS; endothelial nitric oxide synthase; endothelial nitric oxide synthase 3; Endothelial NOS; eNOS; Nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; Nitric oxide synthase, endothelial; NOS type III; NOSIII; OTTHUMP00000213183

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Gene Aliases: 2310065A03Rik; ECNOS; eNOS; NOS; Nos-3; NOS3

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UniProt ID: (Human) P29474, (Mouse) P70313, (Rat) Q62600

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Entrez Gene ID: (Bovine) 287024, (Human) 4846, (Dog) 403784, (Mouse) 18127, (Rat) 24600

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Function(s)

nitric-oxide synthase activity calmodulin binding FMN binding heme binding metal ion binding flavin adenine dinucleotide binding NADP binding actin monomer binding iron ion binding protein binding tetrahydrobiopterin binding arginine binding cadmium ion binding scaffold protein binding actin binding NADPH-hemoprotein reductase activity beta-catenin binding oxidoreductase activity cadherin binding nitric-oxide synthase binding Hsp90 protein binding oxidoreductase protein modifying enzyme

Process(es)

Performance Guarantee

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eNOS Polyclonal Antibody

eNOS Antibody (PA3-031A) in ICC/IF

Product Details

PA3-031A

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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