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eNOS Antibody (MA5-47674) in ICC/IF
Immunocytochemistry analysis of eNOS in paraformaldehyde-fixed and NP-40-permeabilized human umbilical vein endothelial cells. The cells were labeled with mouse eNOS monoclonal antibody (Product # MA5-47674) and rabbit polyclonal eNOS antibody, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
Product Details
MA5-47674
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
M221
Immunogen
Clone (M221) was generated from a recombinant mouse eNOS protein that included amino acids residues in the C-terminal region. This sequence is conserved in human and rat eNOS, and has low homology to other NOS family members.
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2942655
Product Specific Information
The antibody detects a 140 kDa* protein corresponding to eNOS on SDS-PAGE immunoblots of human umbilical vein endothelial cells.
Target Information
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cNOS; Constitutive NOS; e-NOS; EC-NOS; endothelial nitric oxide synthase; endothelial nitric oxide synthase 3; Endothelial NOS; Nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; Nitric oxide synthase, endothelial; NOS type III; NOSIII; OTTHUMP00000213183
Gene Aliases: 2310065A03Rik; ECNOS; eNOS; Nos-3; NOS3
UniProt ID: (Human) P29474, (Mouse) P70313, (Rat) Q62600
Entrez Gene ID: (Human) 4846, (Mouse) 18127, (Rat) 24600
actin monomer binding
nitric-oxide synthase activity
iron ion binding
protein binding
calmodulin binding
FMN binding
heme binding
tetrahydrobiopterin binding
arginine binding
cadmium ion binding
flavin adenine dinucleotide binding
NADP binding
scaffold protein binding
actin binding
NADPH-hemoprotein reductase activity
beta-catenin binding
oxidoreductase activity
cadherin binding
metal ion binding
nitric-oxide synthase binding
Hsp90 protein binding
oxidoreductase
protein modifying enzyme
angiogenesis
ovulation from ovarian follicle
in utero embryonic development
blood vessel remodeling
regulation of sodium ion transport
regulation of the force of heart contraction by chemical signal
regulation of systemic arterial blood pressure by endothelin
arginine catabolic process
nitric oxide biosynthetic process
mitochondrion organization
nitric oxide mediated signal transduction
regulation of blood pressure
negative regulation of cell proliferation
response to heat
negative regulation of platelet activation
negative regulation of muscle hyperplasia
smooth muscle hyperplasia
removal of superoxide radicals
lung development
positive regulation of guanylate cyclase activity
lipopolysaccharide-mediated signaling pathway
response to fluid shear stress
negative regulation of potassium ion transport
endothelial cell migration
cell redox homeostasis
positive regulation of angiogenesis
negative regulation of blood pressure
positive regulation of vasodilation
regulation of blood vessel size
regulation of nitric-oxide synthase activity
negative regulation of hydrolase activity
negative regulation of calcium ion transport
oxidation-reduction process
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
signal transduction
response to estradiol
positive regulation of apoptotic process
negative regulation of smooth muscle cell proliferation
cellular response to mechanical stimulus
cellular response to transforming growth factor beta stimulus
response to hypoxia
female pregnancy
aging
response to mechanical stimulus
response to fructose
response to metal ion
response to lead ion
response to organic cyclic compound
response to activity
response to lipopolysaccharide
response to cytokine
cellular response to heat
response to statin
response to drug
response to amino acid
response to peptide hormone
response to ethanol
response to axon injury
response to corticosterone
response to hyperoxia
response to lipoprotein particle
retina development in camera-type eye
bone development
cellular response to magnesium ion
cellular response to cAMP
cellular response to tumor necrosis factor
cellular response to growth factor stimulus
cellular response to organic cyclic compound
response to candesartan
response to peptide
response to ketone
response to salt
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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