Invitrogen

gamma Catenin Monoclonal Antibody (PG-11E4)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

17 Published Figures

33 References

View all (47) gamma Catenin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

gamma Catenin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 24

gamma Catenin Antibody (13-8500) in ICC/IF

Immunofluorescence analysis of Gamma-Catenin was performed using 70% confluent log phase CACO2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Gamma-Catenin / Plakoglobin (PG-11E4) Mouse Monoclonal Antibody (Product # 13-8500) at 1:250 dilution in0.1%... View More

gamma Catenin Antibody in Western Blot (WB)

gamma Catenin Antibody in Immunohistochemistry (IHC)

gamma Catenin Antibody in Western Blot, Immunoprecipitation (WB, IP)

gamma Catenin Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

13-8500

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250

View 17 publications 17 publications

Immunohistochemistry (IHC)

Assay-dependent

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:250

View 6 publications 6 publications

Immunoprecipitation (IP)

Assay-dependent

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

PG-11E4

Immunogen

Fusion protein consisting of the maltose binding protein fused to full length human plakoglobin protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533040

Target Information

JUP is a major cytoplasmic protein which is the only known constituent common to sub membranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Alterations in Calcium Handling Are a Common Feature in an Arrhythmogenic Cardiomyopathy Cell Model Triggered by Desmosome Genes Loss.

International journal of molecular sciences

Vallverdú-Prats M,Carreras D,Pérez GJ,Campuzano O,Brugada R,Alcalde M

13-8500 was used in Western Blotting to propose that the loss of desmosomal genes provokes alterations in calcium handling, potentially contributing to the development of arrhythmogenic events in ACM.

Fri Jan 20 00:00:00 EST 2023

Premature Termination Codon in 5' Region of Desmoplakin and Plakoglobin Genes May Escape Nonsense-Mediated Decay through the Reinitiation of Translation.

International journal of molecular sciences

Vallverdú-Prats M,Brugada R,Alcalde M

13-8500 was used in Western Blotting to evaluate the likelihood that desmosomal genes carrying 5'-premature termination codon will trigger reinitiation.

Fri Jan 07 00:00:00 EST 2022

The fine-tuning of endoplasmic reticulum stress response and autophagy activation during trophoblast syncytialization.

Cell death & disease

Bastida-Ruiz D,Yart L,Wuillemin C,Ribaux P,Morris N,Epiney M,Martinez de Tejada B,Cohen M

Published figure using gamma Catenin monoclonal antibody (Product # 13-8500) in Immunofluorescence

Mon Sep 09 00:00:00 EDT 2019

Mechanical loading of desmosomes depends on the magnitude and orientation of external stress.

Nature communications

Price AJ,Cost AL,Ungewiß H,Waschke J,Dunn AR,Grashoff C

13-8500 was used in Immunohistochemistry to develop Forster resonance energy transfer-based tension sensors to measure the forces experienced by desmoplakin.

Tue Dec 11 00:00:00 EST 2018

Cardiac Fibro-Adipocyte Progenitors Express Desmosome Proteins and Preferentially Differentiate to Adipocytes Upon Deletion of the Desmoplakin Gene.

Circulation research

Lombardi R,Chen SN,Ruggiero A,Gurha P,Czernuszewicz GZ,Willerson JT,Marian AJ

Published figure using gamma Catenin monoclonal antibody (Product # 13-8500) in Immunocytochemistry

Fri Jun 24 00:00:00 EDT 2016

SuperSILAC Quantitative Proteome Profiling of Murine Middle Ear Epithelial Cell Remodeling with NTHi.

PloS one

Val S,Burgett K,Brown KJ,Preciado D

13-8500 was used in western blot to characterize the murine middle ear epithelial cell remodeling with NTHi by SuperSILAC quantitative proteome profiling

Tue Jul 19 00:00:00 EDT 2016

Glucocorticoids and histone deacetylase inhibitors cooperate to block the invasiveness of basal-like breast cancer cells through novel mechanisms.

Oncogene

Law ME,Corsino PE,Jahn SC,Davis BJ,Chen S,Patel B,Pham K,Lu J,Sheppard B,Nørgaard P,Hong J,Higgins P,Kim JS,Luesch H,Law BK

13-8500 was used in immunocytochemistry and western blot to report that glucocorticoids and HDAC inhibitors cooperate to suppress the invasiveness of breast cancer cells via E-cadherin.

Thu Mar 07 00:00:00 EST 2013

Glucocorticoids and histone deacetylase inhibitors cooperate to block the invasiveness of basal-like breast cancer cells through novel mechanisms.

Oncogene

Law ME,Corsino PE,Jahn SC,Davis BJ,Chen S,Patel B,Pham K,Lu J,Sheppard B,Nørgaard P,Hong J,Higgins P,Kim JS,Luesch H,Law BK

13-8500 was used in immunocytochemistry and western blot to report that glucocorticoids and HDAC inhibitors cooperate to suppress the invasiveness of breast cancer cells via E-cadherin.

Thu Mar 07 00:00:00 EST 2013

Gastro-oesophageal reflux disease is associated with up-regulation of desmosomal components in oesophageal mucosa.

Histopathology

Wex T,Mönkemüller K,Stahr A,Kuester D,Fry LC,Völkel S,Kandulski A,Roessner A,Malfertheiner P

13-8500 was used in immunohistochemistry to examine the role of desmosomal proteins in gastro-oesophageal reflux disease.

Wed Feb 01 00:00:00 EST 2012

Assessment of desmosomal components (desmoglein 1-3, plakoglobin) in cardia mucosa in relation to gastroesophageal reflux disease and Helicobacter pylori infection.

Human pathology

Wex T,Kuester D,Mönkemüller K,Stahr A,Fry LC,Kandulski A,Kropf S,Roessner A,Malfertheiner P

13-8500 was used in immunohistochemistry to examine the contribution of the desmosomal compartment to gastroesophageal reflux disease.

Mon Oct 01 00:00:00 EDT 2012

EpCAM contributes to formation of functional tight junction in the intestinal epithelium by recruiting claudin proteins.

Developmental biology

Lei Z,Maeda T,Tamura A,Nakamura T,Yamazaki Y,Shiratori H,Yashiro K,Tsukita S,Hamada H

13-8500 was used in immunohistochemistry (frozen) to report that mice lacking EpCAM have intestinal barrier defects and die shortly after birth as a result of intestinal erosion.

Thu Nov 15 00:00:00 EST 2012

Functional effects of the TMEM43 Ser358Leu mutation in the pathogenesis of arrhythmogenic right ventricular cardiomyopathy.

BMC medical genetics

Rajkumar R,Sembrat JC,McDonough B,Seidman CE,Ahmad F

Published figure using gamma Catenin monoclonal antibody (Product # 13-8500) in Western Blot

Thu Mar 29 00:00:00 EDT 2012

Nuclear plakoglobin is essential for differentiation of cardiac progenitor cells to adipocytes in arrhythmogenic right ventricular cardiomyopathy.

Circulation research

Lombardi R,da Graca Cabreira-Hansen M,Bell A,Fromm RR,Willerson JT,Marian AJ

13-8500 was used in Western Blotting to assess the pathogenic role of plakoglobin in adipogenesis in Arrhythmogenic right ventricular cardiomyopathy.

Fri Dec 09 00:00:00 EST 2011

SOX4 interacts with plakoglobin in a Wnt3a-dependent manner in prostate cancer cells.

BMC cell biology

Lai YH,Cheng J,Cheng D,Feasel ME,Beste KD,Peng J,Nusrat A,Moreno CS

Published figure using gamma Catenin monoclonal antibody (Product # 13-8500) in Immunofluorescence

Sat Nov 19 00:00:00 EST 2011

SOX4 interacts with plakoglobin in a Wnt3a-dependent manner in prostate cancer cells.

BMC cell biology

Lai YH,Cheng J,Cheng D,Feasel ME,Beste KD,Peng J,Nusrat A,Moreno CS

Published figure using gamma Catenin monoclonal antibody (Product # 13-8500) in Immunofluorescence

Sat Nov 19 00:00:00 EST 2011

SOX4 interacts with plakoglobin in a Wnt3a-dependent manner in prostate cancer cells.

BMC cell biology

Lai YH,Cheng J,Cheng D,Feasel ME,Beste KD,Peng J,Nusrat A,Moreno CS

Published figure using gamma Catenin monoclonal antibody (Product # 13-8500) in Immunofluorescence

Sat Nov 19 00:00:00 EST 2011

Genetic fate mapping identifies second heart field progenitor cells as a source of adipocytes in arrhythmogenic right ventricular cardiomyopathy.

Circulation research

Lombardi R,Dong J,Rodriguez G,Bell A,Leung TK,Schwartz RJ,Willerson JT,Brugada R,Marian AJ

13-8500 was used in Immunoprecipitation to identify the cellular origin of excess adipocytes, the mechanism and basis for predominant involvement of the right ventricle in arrhythmogenic right ventricular cardiomyopathy.

Fri May 08 00:00:00 EDT 2009

Long-term, multilineage hematopoiesis occurs in the combined absence of beta-catenin and gamma-catenin.

Blood

Jeannet G,Scheller M,Scarpellino L,Duboux S,Gardiol N,Back J,Kuttler F,Malanchi I,Birchmeier W,Leutz A,Huelsken J,Held W

13-8500 was used in western blot to investigate whether hematopoietic cells transduce canonical Wnt signals in the absence of beta- and gamma-catenin

Tue Jan 01 00:00:00 EST 2008

Characterization of a novel type of adherens junction in meningiomas and the derived cell line HBL-52.

Cell and tissue research

Akat K,Bleck CK,Lee YM,Haselmann-Weiss U,Kartenbeck J

13-8500 was used in immunocytochemistry identify and characterize a new type of adherens junction in human meningiomas

Fri Feb 01 00:00:00 EST 2008

Rab4A GTPase catenin interactions are involved in cell junction dynamics in the testis.

Journal of andrology

Mruk DD,Lau AS,Sarkar O,Xia W

13-8500 was used in Western Blotting to suggest that Rab4A participates in adherens junction dynamics in the testis.

Thu Dec 27 00:00:00 EST 2007

Adjudin-mediated junction restructuring in the seminiferous epithelium leads to displacement of soluble guanylate cyclase from adherens junctions.

Journal of cellular physiology

Sarkar O,Xia W,Mruk DD

13-8500 was used in immunoprecipitation and western blot to determine the role of soluble guanylate cyclase in junction restructuring of the seminiferous epithelium of rat testis

Sat Jul 01 00:00:00 EDT 2006

Adjudin-mediated junction restructuring in the seminiferous epithelium leads to displacement of soluble guanylate cyclase from adherens junctions.

Journal of cellular physiology

Sarkar O,Xia W,Mruk DD

13-8500 was used in immunoprecipitation and western blot to determine the role of soluble guanylate cyclase in junction restructuring of the seminiferous epithelium of rat testis

Sat Jul 01 00:00:00 EDT 2006

Spink5-deficient mice mimic Netherton syndrome through degradation of desmoglein 1 by epidermal protease hyperactivity.

Nature genetics

Descargues P,Deraison C,Bonnart C,Kreft M,Kishibe M,Ishida-Yamamoto A,Elias P,Barrandon Y,Zambruno G,Sonnenberg A,Hovnanian A

Sat Jan 01 00:00:00 EST 2005

Spink5-deficient mice mimic Netherton syndrome through degradation of desmoglein 1 by epidermal protease hyperactivity.

Nature genetics

Descargues P,Deraison C,Bonnart C,Kreft M,Kishibe M,Ishida-Yamamoto A,Elias P,Barrandon Y,Zambruno G,Sonnenberg A,Hovnanian A

Sat Jan 01 00:00:00 EST 2005

Modulating the strength of cadherin adhesion: evidence for a novel adhesion complex.

Journal of cell science

Kim YJ,Sauer C,Testa K,Wahl JK,Svoboda RA,Johnson KR,Wheelock MJ,Knudsen KA

13-8500 was used in immunocytochemistry and western blot to identify and characterize a novel classical cadherin adhesion system

Thu Sep 01 00:00:00 EDT 2005

Mispolarization of desmosomal proteins and altered intercellular adhesion in autosomal dominant polycystic kidney disease.

American journal of physiology. Renal physiology

Silberberg M,Charron AJ,Bacallao R,Wandinger-Ness A

Published figure using gamma Catenin monoclonal antibody (Product # 13-8500) in Western Blot

Wed Jun 01 00:00:00 EDT 2005

Mispolarization of desmosomal proteins and altered intercellular adhesion in autosomal dominant polycystic kidney disease.

American journal of physiology. Renal physiology

Silberberg M,Charron AJ,Bacallao R,Wandinger-Ness A

Published figure using gamma Catenin monoclonal antibody (Product # 13-8500) in Western Blot

Wed Jun 01 00:00:00 EDT 2005

The duality of beta-catenin function: a requirement in lens morphogenesis and signaling suppression of lens fate in periocular ectoderm.

Developmental biology

Smith AN,Miller LA,Song N,Taketo MM,Lang RA

13-8500 was used in Immunohistochemistry-immunofluorescence to investigate the cytoskeletal and signalling roles of beta-catenin during the early phases of lens development.

Thu Sep 15 00:00:00 EDT 2005

Identification of a role for beta-catenin in the establishment of a bipolar mitotic spindle.

The Journal of biological chemistry

Kaplan DD,Meigs TE,Kelly P,Casey PJ

Fri Mar 19 00:00:00 EST 2004

Identification of a role for beta-catenin in the establishment of a bipolar mitotic spindle.

The Journal of biological chemistry

Kaplan DD,Meigs TE,Kelly P,Casey PJ

Fri Mar 19 00:00:00 EST 2004

C-terminal-binding protein corepresses epithelial and proapoptotic gene expression programs.

Proceedings of the National Academy of Sciences of the United States of America

Grooteclaes M,Deveraux Q,Hildebrand J,Zhang Q,Goodman RH,Frisch SM

13-8500 was used in western blot to examine if the epithelial-to-mesenchymal transitions and the acquisition of apoptosis resistance in cancer cells are dependent or independent events.

Tue Apr 15 00:00:00 EDT 2003

Hantavirus infection induces the expression of RANTES and IP-10 without causing increased permeability in human lung microvascular endothelial cells.

Journal of virology

Sundstrom JB,McMullan LK,Spiropoulou CF,Hooper WC,Ansari AA,Peters CJ,Rollin PE

13-8500 was used in Western Blotting to reveal a possible mechanism by which hantavirus infection and a TH1 immune response can cooperate to synergistically enhance chemokine expression by HMVEC-Ls and trigger immune-mediated increases in vascular permeability.

Sun Jul 01 00:00:00 EDT 2001

A comparative evaluation of beta-catenin and plakoglobin signaling activity.

Oncogene

Williams BO,Barish GD,Klymkowsky MW,Varmus HE

13-8500 was used in Western Blot to compare the differences between beta-catenin and plakoglobin.

Thu Nov 23 00:00:00 EST 2000

Tumor-derived mutated E-cadherin influences beta-catenin localization and increases susceptibility to actin cytoskeletal changes induced by pervanadate.

Cell adhesion and communication

Luber B,Candidus S,Handschuh G,Mentele E,Hutzler P,Feller S,Voss J,Höfler H,Becker KF

13-8500 was used in western blot to assess the localization of adherens junction components in cells expressing mutant E-cadherin derivatives.

Mon May 01 00:00:00 EDT 2000

Bioinformatics

Protein Aliases: catenin (cadherin-associated protein), gamma 80kDa; Catenin gamma; Desmoplakin III; Desmoplakin-3; gamma-catenin; Junction plakoglobin; OTTHUMP00000164735

View more View less

Gene Aliases: ARVD12; CTNNG; D930025P04Rik; DP3; DPIII; JUP; PDGB; PG; PKGB

View more View less

UniProt ID: (Human) P14923, (Mouse) Q02257

View more View less

Entrez Gene ID: (Human) 3728, (Mouse) 16480

View more View less

Function(s)

transcription coactivator activity signal transducer activity structural molecule activity structural constituent of cell wall protein binding protein kinase binding protein phosphatase binding protein homodimerization activity alpha-catenin binding cadherin binding cell adhesion molecule binding cell adhesive protein binding involved in bundle of His cell-Purkinje myocyte communication cadherin binding involved in cell-cell adhesion

Process(es)

desmosome assembly cytoskeletal anchoring at plasma membrane signal transduction single organismal cell-cell adhesion cell migration adherens junction organization adherens junction assembly regulation of cell proliferation positive regulation of protein import into nucleus skin development detection of mechanical stimulus positive regulation of sequence-specific DNA binding transcription factor activity protein heterooligomerization endothelial cell-cell adhesion cellular response to indole-3-methanol bundle of His cell-Purkinje myocyte adhesion involved in cell communication regulation of heart rate by cardiac conduction establishment of protein localization to plasma membrane positive regulation of canonical Wnt signaling pathway regulation of ventricular cardiac muscle cell action potential cell adhesion regulation of cell fate specification regulation of nucleic acid-templated transcription

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

gamma Catenin Monoclonal Antibody (PG-11E4)

gamma Catenin Antibody (13-8500) in ICC/IF

Product Details

13-8500

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!