Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
NeoBiotechnologies
gp100/Melanosome/PMEL17/SILV (Melanoma Marker) Recombinant Mouse Monoclonal Antibody (rPMEL17/6821)
Product Details
6490-MSM11-P1
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
rPMEL17/6821
Immunogen
Recombinant full-length human gp100 protein
Conjugate
Form
Liquid
Concentration
200 µg/mL
Purification
Protein A/G
Storage buffer
PBS with 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Antibody is stable for 24 months.
Positive Control: COLO-38 or SK-MEL-28 cells. Human melanoma. Cellular Localization: Cytoplasm
Specificity Comments: The gp100 molecule is a 100kDa glycosylated protein that is cleaved into a small (26kDa) carboxy-terminal fragment and a larger amino- terminal section (60 64 kDa), which is subsequently cleaved to generate 26kDa and 34 38kDa fragments. By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa).
Does not react with dog and rat
Target Information
Melanoma is a malignant tumor of melanocytes which are found predominantly in skin but also in the bowel and the eye (see uveal melanoma). It is one of the rarer types of skin cancer but causes the majority of skin cancer related deaths.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: 95kDa melanocyte-specific secreted glycoprotein; ME20-M; ME20M; Melanocyte lineage specific antigen GP100; melanocyte protein mel 17; Melanocyte protein PMEL; Melanocyte protein Pmel 17; Melanocytes lineage-specific antigen GP100; Melanoma-associated ME20 antigen; melanosomal matrix protein17; P1; P100; Pmel 17; Premelanosome protein; Silver locus protein homolog; silver, mouse, homolog of
Gene Aliases: D12S53E; gp100; ME20; ME20-M; ME20M; P1; P100; PMEL; PMEL17; SI; SIL; SILV
UniProt ID: (Human) P40967
Entrez Gene ID: (Human) 6490
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support