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Product Details
MA5-42837
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
3L0U6
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 2-200 of human iNOS (P35228).
Conjugate
Form
Liquid
Concentration
3 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911978
Product Specific Information
Positive test controls include: RAW264.7.
Immunogen sequence: ACPWKFLFKT KFHQYAMNGE KDINNNVEKA PCATSSPVTQ DDLQYHNLSK QQNESPQPLV ETGKKSPESL VKLDATPLSS PRHVRIKNWG SGMTFQDTLH HKAKGILTCR SKSCLGSIMT PKSLTRGPRD KPTPPDELLP QAIEFVNQYY GSFKEAKIEE HLARVEAVTK EIETTGTYQL TGDELIFATK QAWRNAPRC
Target Information
iNOS (Inducible Nitric oxide, NO, NOS) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. iNOS is expressed in liver and inducible by a combination of lipopolysaccharide and certain cytokines. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. Diseases associated with iNOS dysfunction include achalasia and impotence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hepatocytes; inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; MAC-NOS; Macrophage NOS; nitric oxide synthase 2, inducible, macrophage; Nitric oxide synthase, inducible; nitric oxide synthase-inducible; NOS type II; OTTMUSP00000000202; Peptidyl-cysteine S-nitrosylase NOS2
Gene Aliases: i-NOS; iNOS; Inosl; Nos-2; NOS-II; Nos2; Nos2a
UniProt ID: (Mouse) P29477, (Rat) Q06518
Entrez Gene ID: (Mouse) 18126, (Rat) 24599
actin binding
NADPH-hemoprotein reductase activity
nitric-oxide synthase activity
receptor binding
iron ion binding
protein binding
calmodulin binding
beta-catenin binding
cAMP-dependent protein kinase regulator activity
FMN binding
oxidoreductase activity
protein kinase binding
heme binding
tetrahydrobiopterin binding
arginine binding
protein homodimerization activity
cadherin binding
metal ion binding
flavin adenine dinucleotide binding
nitric-oxide synthase binding
Hsp90 protein binding
amino acid binding
NADP binding
oxidoreductase
protein modifying enzyme
ovulation from ovarian follicle
response to hypoxia
arginine catabolic process
superoxide metabolic process
nitric oxide biosynthetic process
inflammatory response
nitric oxide mediated signal transduction
negative regulation of gene expression
peptidyl-cysteine S-nitrosylation
positive regulation of guanylate cyclase activity
response to lipopolysaccharide
cellular response to drug
regulation of cell proliferation
negative regulation of protein catabolic process
defense response to bacterium
negative regulation of blood pressure
regulation of protein kinase activity
positive regulation of vasodilation
regulation of insulin secretion
positive regulation of killing of cells of other organism
oxidation-reduction process
cellular response to lipopolysaccharide
cellular response to interferon-gamma
endothelial cell proliferation
blood vessel remodeling
signal transduction
G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger
aging
regulation of heart contraction
regulation of blood pressure
response to mechanical stimulus
response to hormone
response to activity
glycoside metabolic process
cGMP-mediated signaling
response to estradiol
response to tumor necrosis factor
intracellular signal transduction
response to immobilization stress
wound healing
positive regulation of apoptotic process
retinoic acid receptor signaling pathway
defense response to Gram-negative bacterium
cellular response to lithium ion
cellular response to vitamin D
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular response to redox state
response to dexamethasone
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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