Invitrogen

iNOS Monoclonal Antibody (4E5)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3 Published Figures

14 References

View all (65) iNOS antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

iNOS Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

iNOS Antibody (MA5-17139) in ICC/IF

Immunofluorescence analysis of iNOS Monoclonal Antibody (4E5) was performed using 70% confluent log phase Hep G2 cells treated with 4 ng/mL of IL-1 beta for 16 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with iNOS Monoclonal Antibody (4E5) (Product # MA5-17139) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secon... View More

iNOS Antibody in Immunohistochemistry (Paraffin) (IHC (P))

iNOS Antibody in Immunohistochemistry (IHC)

Product Image Gallery

iNOS Antibody — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Immunofluorescence analysis of iNOS using iNOS Monoclonal Antibody (4E5) (Product # MA5-17139) shows induction of iNOS in Hep G2 cell line upon IL-1 beta treatment. {TM}

Product Details

MA5-17139

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5 µg/mL

View 2 publications 2 publications

Flow Cytometry (Flow)

1:200-1:400

View 4 publications 4 publications

ELISA (ELISA)

1:10,000

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rabbit, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4E5

Immunogen

Purified recombinant fragment of human NOS2 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538610

Product Specific Information

MA5-17139 targets NOS2 in FACS, IHC, indirect ELISA, and WB applications and shows reactivity with Human and Mouse samples.

The MA5-17139 immunogen is purified recombinant fragment of human NOS2 expressed in E. Coli.

MA5-17139 detects NOS2 which has a predicted molecular weight of approximately 131kDa.

Target Information

iNOS (Inducible Nitric oxide, NO, NOS) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. iNOS is expressed in liver and inducible by a combination of lipopolysaccharide and certain cytokines. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. Diseases associated with iNOS dysfunction include achalasia and impotence.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Expression of Cellular Prion Protein, PrPC, Favors pTau Propagation and Blocks NMDAR Signaling in Primary Cortical Neurons.

Cells

Rivas-Santisteban R,Raïch I,Aguinaga D,Saura CA,Franco R,Navarro G

MA5-17139 was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to identify NMDA-PrPC complexes where PrPC was capable of blocking NMDAR-mediated effects.

Wed Jan 11 00:00:00 EST 2023

CircSLCO3A1 depletion ameliorates lipopolysaccharide-induced inflammation and apoptosis of human pulmonary alveolar epithelial cells through the miR-424-5p/HMGB3 pathway.

Molecular & cellular toxicology

Li Y,Zhang C,Zhao Z

MA5-17139 was used in Western Blotting to investigate the role of circSLCO3A1 in acute lung injury (ALI) and its underlying mechanism, revealing that circSLCO3A1 upregulation in ALI contributes to pulmonary epithelial cell inflammation and apoptosis through the miR-424-5p/HMGB3 axis.

Thu May 25 00:00:00 EDT 2023

MAGL regulates synovial macrophage polarization vis inhibition of mitophagy in osteoarthritic pain.

Molecular medicine reports

Gu C,Chen M,Li X,Geng D,Wang C

MA5-17139 was used in Immunohistochemistry to investigate the potential mechanism of Monoacylglycerol lipase in osteoarthritis pain.

Thu Jun 01 00:00:00 EDT 2023

FcγRIIB potentiates differentiation of myeloid-derived suppressor cells to mediate tumor immunoescape.

Theranostics

Wu L,Xu Y,Zhao H,Zhou Y,Chen Y,Yang S,Lei J,Zhang J,Wang J,Wu Y,Li Y

MA5-17139 was used in Flow Cytometry to uncover an unrecognized regulatory role of the FcγRIIB in abnormal differentiation of MDSCs during cancer development and suggest a potential therapeutic target for anti-tumor therapy.

Thu Mar 10 00:00:00 EST 2022

FcγRIIB potentiates differentiation of myeloid-derived suppressor cells to mediate tumor immunoescape.

Theranostics

Wu L,Xu Y,Zhao H,Zhou Y,Chen Y,Yang S,Lei J,Zhang J,Wang J,Wu Y,Li Y

Thu Mar 10 00:00:00 EST 2022

Riboflavin attenuates tartrazine toxicity in the cerebellar cortex of adult albino rat.

Scientific reports

Ismail OI,Rashed NA

MA5-17139 was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to investigate the impact of tartrazine administration on the cerebellum and to assess the potential role of riboflavin co-administration in the adult male albino rat.

Fri Nov 11 00:00:00 EST 2022

Dual targeting of mTOR/IL-17A and autophagy by fisetin alleviates psoriasis-like skin inflammation.

Frontiers in immunology

Roy T,Banang-Mbeumi S,Boateng ST,Ruiz EM,Chamcheu RN,Kang L,King JA,Walker AL,Nagalo BM,Kousoulas KG,Esnault S,Huang S,Chamcheu JC

MA5-17139 was used in Immunohistochemistry to suggest fisetin as a potential treatment for psoriasis and possibly other inflammatory skin diseases.

Tue Feb 07 00:00:00 EST 2023

Local Delivery of Pirfenidone by PLA Implants Modifies Foreign Body Reaction and Prevents Fibrosis.

Biomedicines

Fayzullin A,Churbanov S,Ignatieva N,Zakharkina O,Tokarev M,Mudryak D,Khristidis Y,Balyasin M,Kurkov A,Golubeva EN,Aksenova NA,Dyuzheva T,Timashev P,Guller A,Shekhter A

MA5-17139 was used in Immunohistochemistry (Paraffin) to understand the possible role of Pirfenidone and polyactic acid in reducing peri-implant fibrosis in post-surgery.

Wed Jul 21 00:00:00 EDT 2021

Local Delivery of Pirfenidone by PLA Implants Modifies Foreign Body Reaction and Prevents Fibrosis.

Biomedicines

Fayzullin A,Churbanov S,Ignatieva N,Zakharkina O,Tokarev M,Mudryak D,Khristidis Y,Balyasin M,Kurkov A,Golubeva EN,Aksenova NA,Dyuzheva T,Timashev P,Guller A,Shekhter A

MA5-17139 was used in Immunohistochemistry (Paraffin) to understand the possible role of Pirfenidone and polyactic acid in reducing peri-implant fibrosis in post-surgery.

Wed Jul 21 00:00:00 EDT 2021

Platelet-Like Fusogenic Liposome-Mediated Targeting Delivery of miR-21 Improves Myocardial Remodeling by Reprogramming Macrophages Post Myocardial Ischemia-Reperfusion Injury.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Tan H,Song Y,Chen J,Zhang N,Wang Q,Li Q,Gao J,Yang H,Dong Z,Weng X,Wang Z,Sun D,Yakufu W,Pang Z,Huang Z,Ge J

MA5-17139 was used in Immunocytochemistry to construct a platelet-like fusogenic liposome (PLPs) to deliver contents to the cytoplasm of target cells.

Sun Aug 01 00:00:00 EDT 2021

RIPK3 Orchestrates Fatty Acid Metabolism in Tumor-Associated Macrophages and Hepatocarcinogenesis.

Cancer immunology research

Wu L,Zhang X,Zheng L,Zhao H,Yan G,Zhang Q,Zhou Y,Lei J,Zhang J,Wang J,Xin R,Jiang L,Peng J,Chen Q,Lam SM,Shui G,Miao H,Li Y

MA5-17139 was used in Flow cytometry/Cell sorting to provide molecular basis for the regulation of RIPK3-mediated, lipid metabolic reprogramming of TAMs, thus highlighting a potential strategy for targeting the immunometabolism of HCC.

Fri May 01 00:00:00 EDT 2020

Short-Chain Fatty Acids Improve Poststroke Recovery via Immunological Mechanisms.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Sadler R,Cramer JV,Heindl S,Kostidis S,Betz D,Zuurbier KR,Northoff BH,Heijink M,Goldberg MP,Plautz EJ,Roth S,Malik R,Dichgans M,Holdt LM,Benakis C,Giera M,Stowe AM,Liesz A

MA5-17139 was used in Immunohistochemistry to test the hypothesis that short-chain fatty acids (SCFAs), key bioactive microbial metabolites, are the missing link along the gut-brain axis and might be able to modulate recovery after experimental stroke.

Wed Jan 29 00:00:00 EST 2020

TRPV4 Plays a Role in Matrix Stiffness-Induced Macrophage Polarization.

Frontiers in immunology

Dutta B,Goswami R,Rahaman SO

MA5-17139 was used in Immunohistochemistry to determine key aspects of the molecular mechanism by which mechanical cues regulate macrophage polarization.

Mon May 31 00:00:00 EDT 2021

TRPV4 Plays a Role in Matrix Stiffness-Induced Macrophage Polarization.

Frontiers in immunology

Dutta B,Goswami R,Rahaman SO

MA5-17139 was used in Immunohistochemistry to determine key aspects of the molecular mechanism by which mechanical cues regulate macrophage polarization.

Mon May 31 00:00:00 EDT 2021

S100A9 Regulates MDSCs-Mediated Immune Suppression via the RAGE and TLR4 Signaling Pathways in Colorectal Carcinoma.

Frontiers in immunology

Huang M,Wu R,Chen L,Peng Q,Li S,Zhang Y,Zhou L,Duan L

MA5-17139 was used in Flow Cytometry to investigate the effect and molecular mechanisms of S100A9 on the accumulation and immunosuppressive function of myeloid-derived suppressor cells in colorectal carcinoma.

Tue Nov 10 00:00:00 EST 2020

A RIPK3-PGE₂ Circuit Mediates Myeloid-Derived Suppressor Cell-Potentiated Colorectal Carcinogenesis.

Cancer research

Yan G,Zhao H,Zhang Q,Zhou Y,Wu L,Lei J,Wang X,Zhang J,Zhang X,Zheng L,Du G,Xiao W,Tang B,Miao H,Li Y

MA5-17139 was used in Flow cytometry/Cell sorting to demonstrate a novel signaling circuit by which RIPK3 and PGE2 regulate tumor immunity.

Mon Oct 01 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: HEP-NOS; Hepatocyte NOS; hepatocytes; inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; MAC-NOS; Macrophage NOS; nitric oxide synthase 2, inducible; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase 2A (inducible, hepatocytes); Nitric oxide synthase, inducible; nitric oxide synthase, macrophage; nitric oxide synthase-inducible; NOS type II; NOS, type II; OTTMUSP00000000202; Peptidyl-cysteine S-nitrosylase NOS2

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Gene Aliases: HEP-NOS; i-NOS; INOS; Inosl; NOS; Nos-2; NOS-II; NOS2; NOS2A

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UniProt ID: (Human) O94994, (Mouse) P29477

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Entrez Gene ID: (Human) 4843, (Mouse) 18126

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Function(s)

nitric-oxide synthase activity receptor binding protein binding calmodulin binding FMN binding heme binding tetrahydrobiopterin binding arginine binding protein homodimerization activity metal ion binding flavin adenine dinucleotide binding NADP binding actin binding NADPH-hemoprotein reductase activity iron ion binding beta-catenin binding cAMP-dependent protein kinase regulator activity oxidoreductase activity protein kinase binding cadherin binding nitric-oxide synthase binding Hsp90 protein binding oxidoreductase

Process(es)

response to hypoxia positive regulation of leukocyte mediated cytotoxicity innate immune response in mucosa arginine catabolic process superoxide metabolic process nitric oxide biosynthetic process nitric oxide mediated signal transduction circadian rhythm response to bacterium negative regulation of gene expression peptidyl-cysteine S-nitrosylation positive regulation of guanylate cyclase activity prostaglandin secretion cellular response to drug regulation of cell proliferation negative regulation of protein catabolic process defense response to bacterium regulation of cellular respiration cell redox homeostasis negative regulation of blood pressure positive regulation of vasodilation regulation of insulin secretion defense response to Gram-negative bacterium positive regulation of killing of cells of other organism oxidation-reduction process cellular response to lipopolysaccharide cellular response to interferon-gamma interleukin-6 secretion interleukin-8 secretion regulation of cytokine production involved in inflammatory response ovulation from ovarian follicle inflammatory response response to lipopolysaccharide regulation of protein kinase activity

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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iNOS Monoclonal Antibody (4E5)

iNOS Antibody (MA5-17139) in ICC/IF

Product Details

MA5-17139

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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