Invitrogen

mCherry Monoclonal Antibody (4B3)

FIGURE: 1 / 1

mCherry Antibody (MA5-32977) in WB

Western blot analysis of mCherry in mCherry fusion protein (1 µg). Sample was incubated with mCherry monoclonal antibody (Product # MA5-32977) using a 1:500-1:10,000 dilution.

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Product Details

MA5-32977

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:10,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

1:200

Product Specifications

Species Reactivity

Tag

Published species

Mouse, Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4B3

Immunogen

Synthetic peptide mCherry-Tag.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2802611

Target Information

MCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct which absorbs at 587 nm and emits at 610 nm. It is resistant to photobleaching and is stable. It matures quickly, with a t 0. 5 of 15 minutes, allowing it to be visualized soon after translation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Neuroprotective properties of anti-apoptotic BCL-2 proteins in 5xFAD mouse model of Alzheimer's disease.

IBRO neuroscience reports

Chernyuk D,Callens M,Polozova M,Gordeev A,Chigriai M,Rakovskaya A,Ilina A,Pchitskaya E,Van den Haute C,Vervliet T,Bultynck G,Bezprozvanny I

MA5-32977 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to indicate that Bcl-2-based strategies hold neuroprotective potential in AD models, though the underlying mechanisms requires further investigation.

Thu Jun 01 00:00:00 EDT 2023

Structural basis for RNA surveillance by the human nuclear exosome targeting (NEXT) complex.

Cell

Puno MR,Lima CD

MA5-32977 was used in Western Blotting to determine cryogenic electron microscopy structures of human nuclear exosome targeting (NEXT) complexes bound to RNA that reveal mechanistic insights to substrate recognition and early steps that precede RNA handover to the exosome.

Thu Jun 09 00:00:00 EDT 2022

Translation and Replication Dynamics of Single RNA Viruses.