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mTOR Antibody (PA1-188) in ICC/IF
Immunofluorescent analysis of mTOR (green) in U-2 OS cells. The cells were fixed with formaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 1% Blocker BSA in PBS (Product # 37525) for 15 minutes, all at room temperature. Cells were stained with an mTOR C-terminal polyclonal antibody (Product # PA1-188) at a dilution of 1:50 in 1% Blocker BSA in PBS (Product # 37525) for 1 hour at room temperature, and then incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody (Pr... View More
Product Details
PA1-188
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to amino acids 2447-2549 of human mTOR expressed in E. coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS with 30% glycerol, 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2539920
Product Specific Information
Western blot analysis of PA1-188 detects mTOR at ~250 kDa in human and non-human primate cells, slightly below the theoretical MW of ~280kDa. Using a Tris-Acetate SDS gel for high molecular weight protein separation, and a nitrocellulose membrane for protein transfer are necessary for optimal WB results.
Immunofluorescence analysis shows cytoplasmic and some nuclear staining of mTOR in human B3 and U-2 OS cells.
Target Information
FRAP1 (mTOR) is a serine/threonine kinase that plays a critical role in cellular growth and proliferation. Perturbations in the mTOR/PI3-kinase/AKT pathway are associated with numerous forms of cancer. FRAP1 is also the target of rapamycin and its analogues, which are currently used as immunosuppressants and cancer therapeutics. Mutations affecting the gene results in Smith-Kingsmore syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP-rapamycin associated protein; FKBP12-rapamycin complex-associated protein; FKBP12-rapamycin complex-associated protein 1; m-TOR; Mammalian target of rapamycin; Mechanistic target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); mTOR; mTORC1; Rapamycin and FKBP12 target 1; rapamycin associated protein FRAP2; Rapamycin target protein 1; Serine/threonine-protein kinase mTOR
Gene Aliases: FRAP; FRAP1; FRAP2; MTOR; RAFT1; RAPT1; SKS
UniProt ID: (Human) P42345
Entrez Gene ID: (Human) 2475
RNA polymerase III type 1 promoter DNA binding
RNA polymerase III type 2 promoter DNA binding
RNA polymerase III type 3 promoter DNA binding
TFIIIC-class transcription factor binding
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
kinase activity
protein kinase binding
protein domain specific binding
ribosome binding
phosphoprotein binding
non-receptor serine/threonine protein kinase
protein modifying enzyme
negative regulation of protein phosphorylation
positive regulation of protein phosphorylation
positive regulation of endothelial cell proliferation
heart morphogenesis
heart valve morphogenesis
regulation of glycogen biosynthetic process
energy reserve metabolic process
'de novo' pyrimidine nucleobase biosynthetic process
DNA repair
protein phosphorylation
response to stress
cell cycle arrest
signal transduction
germ cell development
brain development
cell aging
response to nutrient
long-term memory
visual learning
post-embryonic development
negative regulation of autophagy
positive regulation of lamellipodium assembly
positive regulation of gene expression
positive regulation of myotube differentiation
positive regulation of neuron maturation
negative regulation of muscle atrophy
cell growth
macroautophagy
negative regulation of macroautophagy
phosphorylation
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
spinal cord development
protein catabolic process
positive regulation of actin filament polymerization
T cell costimulation
negative regulation of protein ubiquitination
ruffle organization
regulation of myelination
cellular response to nutrient levels
TOR signaling
regulation of fatty acid beta-oxidation
regulation of response to food
response to insulin
regulation of actin cytoskeleton organization
social behavior
multicellular organism growth
growth
wound healing
response to cocaine
regulation of GTPase activity
response to amino acid
response to morphine
regulation of carbohydrate utilization
positive regulation of nitric oxide biosynthetic process
regulation of osteoclast differentiation
positive regulation of translation
negative regulation of cell size
regulation of protein kinase activity
positive regulation of transcription from RNA polymerase III promoter
protein autophosphorylation
positive regulation of lipid biosynthetic process
phosphatidylinositol-mediated signaling
mRNA stabilization
positive regulation of smooth muscle cell proliferation
positive regulation of oligodendrocyte differentiation
positive regulation of peptidyl-tyrosine phosphorylation
voluntary musculoskeletal movement
positive regulation of stress fiber assembly
negative regulation of NFAT protein import into nucleus
positive regulation of protein kinase B signaling
cardiac muscle cell development
cardiac muscle contraction
maternal process involved in female pregnancy
positive regulation of glial cell proliferation
positive regulation of dendritic spine development
positive regulation of cell growth involved in cardiac muscle cell development
cellular response to hypoxia
regulation of brown fat cell differentiation
regulation of membrane permeability
regulation of cellular response to heat
positive regulation of neuron death
positive regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter
positive regulation of eating behavior
positive regulation of cholangiocyte proliferation
positive regulation of sensory perception of pain
negative regulation of cholangiocyte apoptotic process
positive regulation of granulosa cell proliferation
positive regulation of skeletal muscle hypertrophy
negative regulation of iodide transmembrane transport
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