Invitrogen

mTOR Monoclonal Antibody (215Q18)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

10 Published Figures

13 References

View all (96) mTOR antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

mTOR Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

mTOR Antibody (AHO1232) in ICC/IF

Immunofluorescent analysis of mTOR was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with mTOR Mouse monoclonal Antibody (Product # AHO1232) at 2 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at roo... View More

mTOR Antibody in Immunohistochemistry (Paraffin) (IHC (P))

mTOR Antibody in Immunohistochemistry (IHC)

mTOR Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

mTOR Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A reduced signal was observed for target protein in HeLa Cas9 cell line transduced with mTOR Lentiviral sgRNA compared to control cell line using Anti-mTOR Monoclonal Antibody (215Q18) (Product # AHO1232). {KO}

Product Details

AHO1232

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

Immunocytochemistry (ICC/IF)

1:100-1:500

View 3 publications 3 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

215Q18

Immunogen

Recombinant fragment of human mTOR expressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536329

Target Information

FRAP1 (mTOR) is a serine/threonine kinase that plays a critical role in cellular growth and proliferation. Perturbations in the mTOR/PI3-kinase/AKT pathway are associated with numerous forms of cancer. FRAP1 is also the target of rapamycin and its analogues, which are currently used as immunosuppressants and cancer therapeutics. Mutations affecting the gene results in Smith-Kingsmore syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nicorandil mitigates amiodarone-induced pulmonary toxicity and fibrosis in association with the inhibition of lung TGF-β1/PI3K/Akt1-p/mTOR axis in rats.

Clinical and experimental pharmacology & physiology

Harb I,Ashour H,Rashed LA,Mostafa A,Samir M,Aboulhoda BE,El-Hanbuli H,Rashwan E,Mahmoud H

Published figure using mTOR monoclonal antibody (Product # AHO1232) in Western Blot

Sun Jan 01 00:00:00 EST 2023

Nicorandil mitigates amiodarone-induced pulmonary toxicity and fibrosis in association with the inhibition of lung TGF-β1/PI3K/Akt1-p/mTOR axis in rats.

Clinical and experimental pharmacology & physiology

Harb I,Ashour H,Rashed LA,Mostafa A,Samir M,Aboulhoda BE,El-Hanbuli H,Rashwan E,Mahmoud H

Published figure using mTOR monoclonal antibody (Product # AHO1232) in Western Blot

Sun Jan 01 00:00:00 EST 2023

Berberine Inhibited Growth and Migration of Human Colon Cancer Cell Lines by Increasing Phosphatase and Tensin and Inhibiting Aquaporins 1, 3 and 5 Expressions.

Molecules (Basel, Switzerland)

Tarawneh N,Hamadneh L,Abu-Irmaileh B,Shraideh Z,Bustanji Y,Abdalla S

AHO1232 was used in Western Blotting to investigate the anti-cancer effect of berberine on HT-29, SW-480 and HCT-116 human CRC cell lines.

Sat Apr 29 00:00:00 EDT 2023

Berberine Inhibited Growth and Migration of Human Colon Cancer Cell Lines by Increasing Phosphatase and Tensin and Inhibiting Aquaporins 1, 3 and 5 Expressions.

Molecules (Basel, Switzerland)

Tarawneh N,Hamadneh L,Abu-Irmaileh B,Shraideh Z,Bustanji Y,Abdalla S

AHO1232 was used in Western Blotting to investigate the anti-cancer effect of berberine on HT-29, SW-480 and HCT-116 human CRC cell lines.

Sat Apr 29 00:00:00 EDT 2023

SCA^® Slows the Decline of Functional Parameters Associated with Senescence in Skin Cells.

International journal of molecular sciences

Castro B,de Paz N,González S,Rodríguez-Luna A

Published figure using mTOR monoclonal antibody (Product # AHO1232) in Immunocytochemistry

Fri Jun 10 00:00:00 EDT 2022

Discovery of a novel potentially transforming somatic mutation in CSF2RB gene in breast cancer.

Cancer medicine

Rashid M,Ali R,Almuzzaini B,Song H,AlHallaj A,Abdulkarim AA,Mohamed Baz O,Al Zahrani H,Mustafa Sabeena M,Alharbi W,Hussein M,Boudjelal M

AHO1232 was used in Western Blotting to report the discovery of a mutation in the CSF2RB gene of a breast cancer patient and the cell line KAIMRC1.

Mon Nov 01 00:00:00 EDT 2021

Discovery of a novel potentially transforming somatic mutation in CSF2RB gene in breast cancer.

Cancer medicine

Rashid M,Ali R,Almuzzaini B,Song H,AlHallaj A,Abdulkarim AA,Mohamed Baz O,Al Zahrani H,Mustafa Sabeena M,Alharbi W,Hussein M,Boudjelal M

AHO1232 was used in Western Blotting to report the discovery of a mutation in the CSF2RB gene of a breast cancer patient and the cell line KAIMRC1.

Mon Nov 01 00:00:00 EDT 2021

Isolation and Establishment of a Highly Proliferative, Cancer Stem Cell-Like, and Naturally Immortalized Triple-Negative Breast Cancer Cell Line, KAIMRC2.

Cells

Ali R,Al Zahrani H,Barhoumi T,Alhallaj A,Mashhour A,Alshammari MA,Alshawakir YA,Baz O,Alanazi AH,Khan AL,Al Nikhli H,Al Balwi MA,Al Riyees L,Boudjelal M

AHO1232 was used in Western Blot to report the establishment of a naturally immortalized highly tumorigenic and triple-negative breast cancer cell line, KAIMRC2.

Mon May 24 00:00:00 EDT 2021

Isolation and Establishment of a Highly Proliferative, Cancer Stem Cell-Like, and Naturally Immortalized Triple-Negative Breast Cancer Cell Line, KAIMRC2.

Cells

Ali R,Al Zahrani H,Barhoumi T,Alhallaj A,Mashhour A,Alshammari MA,Alshawakir YA,Baz O,Alanazi AH,Khan AL,Al Nikhli H,Al Balwi MA,Al Riyees L,Boudjelal M

AHO1232 was used in Western Blot to report the establishment of a naturally immortalized highly tumorigenic and triple-negative breast cancer cell line, KAIMRC2.

Mon May 24 00:00:00 EDT 2021

UPF2 leads to degradation of dendritically targeted mRNAs to regulate synaptic plasticity and cognitive function.

Molecular psychiatry

Notaras M,Allen M,Longo F,Volk N,Toth M,Li Jeon N,Klann E,Colak D

AHO1232 was used in Immunohistochemistry to establish that UPF2 regulates synaptic plasticity, cognition, and local protein synthesis in dendrites, providing fundamental insight into the neuron-specific function of NMD within the brain.

Tue Dec 01 00:00:00 EST 2020

Interaction of YAP1 and mTOR promotes bladder cancer progression.

International journal of oncology

Xu M,Gu M,Zhou J,Da J,Wang Z

Published figure using mTOR monoclonal antibody (Product # AHO1232) in Immunofluorescence

Wed Jan 01 00:00:00 EST 2020

Interaction of YAP1 and mTOR promotes bladder cancer progression.

International journal of oncology

Xu M,Gu M,Zhou J,Da J,Wang Z

Published figure using mTOR monoclonal antibody (Product # AHO1232) in Immunofluorescence

Wed Jan 01 00:00:00 EST 2020

Interaction of YAP1 and mTOR promotes bladder cancer progression.

International journal of oncology

Xu M,Gu M,Zhou J,Da J,Wang Z

Published figure using mTOR monoclonal antibody (Product # AHO1232) in Immunofluorescence

Wed Jan 01 00:00:00 EST 2020

Interaction of YAP1 and mTOR promotes bladder cancer progression.

International journal of oncology

Xu M,Gu M,Zhou J,Da J,Wang Z

Published figure using mTOR monoclonal antibody (Product # AHO1232) in Immunofluorescence

Wed Jan 01 00:00:00 EST 2020

A Stat6/Pten Axis Links Regulatory T Cells with Adipose Tissue Function.

Cell metabolism

Kälin S,Becker M,Ott VB,Serr I,Hosp F,Mollah MMH,Keipert S,Lamp D,Rohner-Jeanrenaud F,Flynn VK,Scherm MG,Nascimento LFR,Gerlach K,Popp V,Dietzen S,Bopp T,Krishnamurthy P,Kaplan MH,Serrano M,Woods SC,Tripal P,Palmisano R,Jastroch M,Blüher M,Wolfrum C,Weigmann B,Ziegler AG,Mann M,Tschöp MH,Daniel C

AHO1232 was used in Immunocytochemistry-immunoflourescence to study the link between adipose tissue function and regulatory T cells.

Tue Sep 05 00:00:00 EDT 2017

Anti-inflammatory and Antitumor Activity of a Triple Therapy for a Colitis-Related Colorectal Cancer.

Journal of Cancer

Figueroa-González G,García-Castillo V,Coronel-Hernández J,López-Urrutia E,León-Cabrera S,Arias-Romero LE,Terrazas LI,Rodríguez-Sosa M,Campos-Parra AD,Zúñiga-Calzada E,Lopez-Camarillo C,Morales-González F,Jacobo-Herrera NJ,Pérez-Plasencia C

AHO1232 was used in western blot to discuss targeting mTOR-signaling and lactate dehydrogenase A as a strategy to treat colorectal cancer

Fri Apr 08 00:00:00 EDT 2022

Rapamycin reveals an mTOR-independent repression of Kv1.1 expression during epileptogenesis.

Neurobiology of disease

Sosanya NM,Brager DH,Wolfe S,Niere F,Raab-Graham KF

AHO1232 was used in western blot to study mTOR, microRNA miR-129-5p, and Kv1.1 in epilepsy

Thu Jan 01 00:00:00 EST 2015

Bioinformatics

Protein Aliases: angiopoietin-like factor CDT6; FK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP-rapamycin associated protein; FKBP-rapamycin associated protein (FRAP); FKBP-rapamycin-associated protein FRAP; FKBP12-rapamycin complex-associated protein; FKBP12-rapamycin complex-associated protein 1; m-TOR; Mammalian target of rapamycin; Mechanistic target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); mTOR; mTORC1; Rapamycin and FKBP12 target 1; rapamycin and FKBP12 target-1 protein; rapamycin associated protein FRAP2; Rapamycin target protein 1; RAPT1; Serine/threonine-protein kinase mTOR

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Gene Aliases: 2610315D21Rik; AI327068; flat; FRAP; FRAP1; FRAP2; MTOR; RAFT1; RAPT1; SKS

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UniProt ID: (Human) P42345, (Mouse) Q9JLN9, (Rat) P42346

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Entrez Gene ID: (Human) 2475, (Mouse) 56717, (Rat) 56718

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Function(s)

RNA polymerase III type 1 promoter DNA binding RNA polymerase III type 2 promoter DNA binding RNA polymerase III type 3 promoter DNA binding TFIIIC-class transcription factor binding protein kinase activity protein serine/threonine kinase activity protein binding ATP binding kinase activity protein kinase binding protein domain specific binding ribosome binding phosphoprotein binding nucleotide binding drug binding transferase activity transferase activity, transferring phosphorus-containing groups phosphotransferase activity, alcohol group as acceptor protein dimerization activity non-receptor serine/threonine protein kinase protein modifying enzyme

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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mTOR Monoclonal Antibody (215Q18)

mTOR Antibody (AHO1232) in ICC/IF

Product Details

AHO1232

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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