Invitrogen

nNOS Polyclonal Antibody

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

PA1-4121

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues P(19) R K G P P L P N G D T E C(31) of nNOS.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2152594

Product Specific Information

PA1-4121 detects neuronal Nitric Oxide Synthase in human samples.

PA1-4121 has been successfully used in Western blot procedures.

The PA1-4121 immunogen is a synthetic peptide corresponding to residues P(19) R K G P P L P N G D T E C(31) of nNOS.

Target Information

NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: bNOS; bNOS1; Brain NOS; Constitutive NOS; N-NOS; NC-NOS; Neuronal NOS; Nitric oxide synthase 1; nitric oxide synthase 1 (neuronal); Nitric oxide synthase, brain; NOS Type 1; NOS type I; Peptidyl-cysteine S-nitrosylase NOS1; Phospho-bNOS; Phospho-Brain NOS

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Gene Aliases: bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS; NOS1

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UniProt ID: (Human) P29475

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Entrez Gene ID: (Human) 4842

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Function(s)
nitric-oxide synthase activity iron ion binding protein binding calmodulin binding FMN binding sodium channel regulator activity enzyme binding heme binding tetrahydrobiopterin binding arginine binding protein homodimerization activity ion channel binding cadmium ion binding flavin adenine dinucleotide binding NADP binding scaffold protein binding oxidoreductase
Process(es)
response to hypoxia regulation of sodium ion transport arginine catabolic process nitric oxide biosynthetic process striated muscle contraction nitric oxide mediated signal transduction myoblast fusion female pregnancy aging negative regulation of cell proliferation response to heat response to lead ion negative regulation of calcium ion transport into cytosol response to activity peptidyl-cysteine S-nitrosylation positive regulation of guanylate cyclase activity response to lipopolysaccharide response to vitamin E multicellular organismal response to stress positive regulation of histone acetylation response to nicotine neurotransmitter biosynthetic process exogenous drug catabolic process negative regulation of apoptotic process negative regulation of potassium ion transport response to peptide hormone response to estrogen cell redox homeostasis response to ethanol negative regulation of blood pressure negative regulation of heart contraction positive regulation of transcription, DNA-templated negative regulation of vasoconstriction positive regulation of vasodilation positive regulation of transcription from RNA polymerase II promoter negative regulation of insulin secretion behavioral response to cocaine regulation of neurogenesis negative regulation of hydrolase activity negative regulation of cytosolic calcium ion concentration negative regulation of serotonin uptake negative regulation of calcium ion transport regulation of sensory perception of pain oxidation-reduction process regulation of cardiac muscle contraction regulation of ryanodine-sensitive calcium-release channel activity cellular response to mechanical stimulus cellular response to growth factor stimulus response to nitric oxide positive regulation of the force of heart contraction positive regulation of long-term synaptic potentiation negative regulation of adrenergic receptor signaling pathway involved in heart process positive regulation of adrenergic receptor signaling pathway involved in heart process positive regulation of neuron death positive regulation of sodium ion transmembrane transport regulation of calcium ion transmembrane transport via high voltage-gated calcium channel regulation of cardiac conduction
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