Invitrogen

nNOS Monoclonal Antibody (3G6B10)

6 Published Figures

22 References

View all (25) nNOS antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (6)

nNOS Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 8

nNOS Antibody (37-2800) in IHC (P)

Immunohistochemistry analysis of nNOS showing staining in the cytoplasm of paraffin-embedded human kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, was... View More

nNOS Antibody in Immunohistochemistry (IHC)

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Product Details

37-2800

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

3G6B10

Immunogen

Recombinant protein derived from the N-terminal region of rat nNOS protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533308

Target Information

NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inducible over-expression of cardiac Nos1ap causes short QT syndrome in transgenic mice.

FEBS open bio

Jänsch M,Lubomirov LT,Trum M,Williams T,Schmitt J,Schuh K,Qadri F,Maier LS,Bader M,Ritter O

Published figure using nNOS monoclonal antibody (Product # 37-2800) in Western Blot

Sun Jan 01 00:00:00 EST 2023

In Vitro Generation of Posterior Motor Neurons from Human Pluripotent Stem Cells.

Current protocols

Wind M,Tsakiridis A

37-2800 was used in Immunocytochemistry to utilise human pluripotent stem cells for generation of thoracic spinal cord cells and motor neurons.

Wed Sep 01 00:00:00 EDT 2021

Defining the signalling determinants of a posterior ventral spinal cord identity in human neuromesodermal progenitor derivatives.

Development (Cambridge, England)

Wind M,Gogolou A,Manipur I,Granata I,Butler L,Andrews PW,Barbaric I,Ning K,Guarracino MR,Placzek M,Tsakiridis A

37-2800 was used in ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry to investigate induction of neuromesodermal progenitors.

Tue Mar 23 00:00:00 EDT 2021

Stenosis coexists with compromised α1-adrenergic contractions in the ascending aorta of a mouse model of Williams-Beuren syndrome.

Scientific reports

Jiménez-Altayó F,Ortiz-Romero P,Puertas-Umbert L,Dantas AP,Pérez B,Vila E,D'Ocon P,Campuzano V

Published figure using nNOS monoclonal antibody (Product # 37-2800) in Western Blot

Tue Jan 21 00:00:00 EST 2020

Neuromodulatory Action of Picomolar Extracellular Aβ42 Oligomers on Presynaptic and Postsynaptic Mechanisms Underlying Synaptic Function and Memory.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Gulisano W,Melone M,Ripoli C,Tropea MR,Li Puma DD,Giunta S,Cocco S,Marcotulli D,Origlia N,Palmeri A,Arancio O,Conti F,Grassi C,Puzzo D

37-2800 was used in Western Blotting to show that extracellular pM oAΒ42 increases neurotransmitter release, number of docked vesicles, postsynaptic density length, and expression of plasticity-related proteins leading to the conversion of early-LTP into late-LTP and of short-term into long-term memory.

Wed Jul 24 00:00:00 EDT 2019

Preventive Effects of Different Fermentation Times of Shuidouchi on Diphenoxylate-Induced Constipation in Mice.

Foods (Basel, Switzerland)

Chen L,Zhang J,Suo H,Wang W,Wang H,Zhang Y,Hu Q,Zhao X,Li J

37-2800 was used in Western Blotting to compare the prevention effects of Shuidouchi with different fermentation times on constipation in mice.

Fri Mar 01 00:00:00 EST 2019

NOS3 Inhibition Confers Post-Ischemic Protection to Young and Aging White Matter Integrity by Conserving Mitochondrial Dynamics and Miro-2 Levels.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Bastian C,Zaleski J,Stahon K,Parr B,McCray A,Day J,Brunet S,Baltan S

37-2800 was used in Western Blotting to investigate whether nitric oxide synthase inhibition provides post-ischaemic functional recovery by preserving mitochondria.

Wed Jul 11 00:00:00 EDT 2018

NOS3 Inhibition Confers Post-Ischemic Protection to Young and Aging White Matter Integrity by Conserving Mitochondrial Dynamics and Miro-2 Levels.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Bastian C,Zaleski J,Stahon K,Parr B,McCray A,Day J,Brunet S,Baltan S

37-2800 was used in Western Blotting to investigate whether nitric oxide synthase inhibition provides post-ischaemic functional recovery by preserving mitochondria.

Wed Jul 11 00:00:00 EDT 2018

The role of AMP-activated protein kinase in the expression of the dystrophin-associated protein complex in skeletal muscle.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Dial AG,Rooprai P,Lally JS,Bujak AL,Steinberg GR,Ljubicic V

37-2800 was used in Western Blotting to etermine whether AMPK was necessary for the maintenance of DAPC expression in skeletal muscle.

Fri Jun 01 00:00:00 EDT 2018

Inhibition of NLRP3 Inflammasome Prevents LPS-Induced Inflammatory Hyperalgesia in Mice: Contribution of NF-κB, Caspase-1/11, ASC, NOX, and NOS Isoforms.

Inflammation

Dolunay A,Senol SP,Temiz-Resitoglu M,Guden DS,Sari AN,Sahan-Firat S,Tunctan B

372800 was used in western blot to test if inhibition of the NLRP3 inflammasome prevents inflammatory hyperalgesia induced by lipopolysaccharide

Sat Apr 01 00:00:00 EDT 2017

Increased expression of carbon monoxide-producing enzymes in the MPOA after sexual experience in male rats.

Physiology & behavior

Robison CL,McHenry JA,Hull EM

37-2800 was used in immunohistochemistry to study the effect of carbon monoxide on the medial preoptic area

Wed Mar 15 00:00:00 EDT 2017

Intracallosal neuronal nitric oxide synthase neurons colocalize with neurokinin 1 substance P receptor in the rat.

The Journal of comparative neurology

Barbaresi P,Mensà E,Lariccia V,Desiato G,Fabri M,Gratteri S

37-2800 was used in immunohistochemistry to examine the localization of neurokinin 1 substance P receptor in rat nitric oxide synthase neurons

Sun Mar 01 00:00:00 EST 2015

Inhibition of the cardiac Na⁺ channel Nav1.5 by carbon monoxide.

The Journal of biological chemistry

Elies J,Dallas ML,Boyle JP,Scragg JL,Duke A,Steele DS,Peers C

37-2800 was used in Immunocytochemistry-immunoflourescence to demonstrate that carbon monoxide inhibits peak human Nav1.5 current amplitude without activating the late Na(+) current observed in native tissue.

Fri Jun 06 00:00:00 EDT 2014

Differential distribution of parvalbumin- and calbindin-D28K-immunoreactive neurons in the rat periaqueductal gray matter and their colocalization with enzymes producing nitric oxide.

Brain research bulletin

Barbaresi P,Mensà E,Lariccia V,Pugnaloni A,Amoroso S,Fabri M

37-2800 was used in western blot to study the distribution, colocalization with enzymes producing nitric oxide, and the synaptic organization of neurons containing parvalbumin and calbindin-D28K in the rat periaqueductal gray matter.

Tue Oct 01 00:00:00 EDT 2013

Functional evidence for nitric oxide production by skeletal-muscle mitochondria from lipopolysaccharide-treated mice.

Mitochondrion

Aguirre E,López-Bernardo E,Cadenas S

37-2800 was used in western blot to test if there is a mitochondrially localized nitric oxide synthase.

Sun Jan 01 00:00:00 EST 2012

Correlation between hippocampal levels of neural, epithelial and inducible NOS and spatial learning skills in rats.

Behavioural brain research

Gökçek-Saraç Ç,Karakurt S,Adalı O,Jakubowska-Doğru E

37-2800 was used in western blot to elucidate the role of different nitric oxide synthase isoforms in hippocampus-dependent forms of learning.

Sat Dec 01 00:00:00 EST 2012

Immunocytochemical localization of calretinin-containing neurons in the rat periaqueductal gray and colocalization with enzymes producing nitric oxide: a double, double-labeling study.

Synapse (New York, N.Y.)

Barbaresi P,Quaranta A,Amoroso S,Mensà E,Fabri M

37-2800 was used in western blot to determine the localization of calcium-binding protein calretinin and of enzymes producing nitric oxide in the rat periaqueductal gray matter.

Sun Apr 01 00:00:00 EDT 2012

Altered sodium channel-protein associations in critical illness myopathy.

Skeletal muscle

Kraner SD,Novak KR,Wang Q,Peng J,Rich MM

Published figure using nNOS monoclonal antibody (Product # 37-2800) in Immunofluorescence

Thu Aug 30 00:00:00 EDT 2012

Altered sodium channel-protein associations in critical illness myopathy.

Skeletal muscle

Kraner SD,Novak KR,Wang Q,Peng J,Rich MM

Published figure using nNOS monoclonal antibody (Product # 37-2800) in Immunofluorescence

Thu Aug 30 00:00:00 EDT 2012

Renal NOS activity, expression, and localization in male and female spontaneously hypertensive rats.

American journal of physiology. Regulatory, integrative and comparative physiology

Sullivan JC,Pardieck JL,Hyndman KA,Pollock JS

Fri Jan 01 00:00:00 EST 2010

The nitric oxide synthase family of proteins.

Journal of vascular research

Sessa WC

Thu May 12 00:00:00 EDT 1994

The nitric oxide synthase family of proteins.

Journal of vascular research

Sessa WC

Thu May 12 00:00:00 EDT 1994

Bioinformatics

Protein Aliases: bNOS; bNOS1; Brain NOS; Constitutive NOS; N-NOS; NC-NOS; Neuronal NOS; nitric oxidase synthase; Nitric oxide synthase 1; nitric oxide synthase 1 (neuronal); Nitric oxide synthase, brain; nNOS; NOS Type 1; NOS type I; Peptidyl-cysteine S-nitrosylase NOS1; Phospho-bNOS; Phospho-Brain NOS

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Gene Aliases: 2310005C01Rik; Bnos; IHPS1; N-NOS; NC-NOS; nNOS; NO; NOS; Nos-1; NOS-I; NOS1

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UniProt ID: (Human) P29475, (Rat) P29476, (Mouse) Q9Z0J4

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Entrez Gene ID: (Human) 4842, (Rat) 24598, (Mouse) 18125

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Function(s)

nitric-oxide synthase activity iron ion binding protein binding calmodulin binding FMN binding sodium channel regulator activity enzyme binding heme binding tetrahydrobiopterin binding arginine binding protein homodimerization activity ion channel binding cadmium ion binding flavin adenine dinucleotide binding NADP binding scaffold protein binding NADPH-hemoprotein reductase activity amino acid binding ATPase binding oxidoreductase

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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nNOS Monoclonal Antibody (3G6B10)

nNOS Antibody (37-2800) in IHC (P)

Product Details

37-2800

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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