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nNOS Antibody (MA5-46909) in ICC/IF
Immunofluorescent analysis of nNOS in DIV10 rat E18 primary cortical neuron cells. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: nNOS stained by nNOS monoclonal antibody (Product # MA5-46909) diluted at 1:500. Red: Tau, stained by Tau antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
Product Details
MA5-46909
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
GT5978
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of Rat nNOS. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2937981
Product Specific Information
Store as concentrated solution.
Centrifuge briefly prior to opening vial.
Target Information
NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bNOS; bNOS1; Brain NOS; Constitutive NOS; N-NOS; NC-NOS; Neuronal NOS; nitric oxidase synthase; Nitric oxide synthase 1; Nitric oxide synthase, brain; nNOS; NOS Type 1; NOS type I; Peptidyl-cysteine S-nitrosylase NOS1; Phospho-bNOS; Phospho-Brain NOS
Gene Aliases: 2310005C01Rik; Bnos; N-NOS; NC-NOS; nNOS; NO; NOS; Nos-1; NOS-I; Nos1
UniProt ID: (Rat) P29476, (Mouse) Q9Z0J4
Entrez Gene ID: (Rat) 24598, (Mouse) 18125
NADPH-hemoprotein reductase activity
nitric-oxide synthase activity
iron ion binding
protein binding
calmodulin binding
FMN binding
amino acid binding
sodium channel regulator activity
enzyme binding
heme binding
protein homodimerization activity
ion channel binding
cadmium ion binding
flavin adenine dinucleotide binding
NADP binding
ATPase binding
scaffold protein binding
oxidoreductase
response to hypoxia
regulation of sodium ion transport
arginine catabolic process
nitric oxide biosynthetic process
muscle contraction
striated muscle contraction
nitric oxide mediated signal transduction
female pregnancy
aging
regulation of heart contraction
negative regulation of cell proliferation
response to heat
response to organonitrogen compound
response to lead ion
response to organic cyclic compound
response to activity
peptidyl-cysteine S-nitrosylation
positive regulation of guanylate cyclase activity
response to nutrient levels
response to lipopolysaccharide
negative regulation of peptidyl-serine phosphorylation
response to vitamin E
multicellular organismal response to stress
positive regulation of histone acetylation
response to nicotine
exogenous drug catabolic process
negative regulation of apoptotic process
negative regulation of potassium ion transport
response to peptide hormone
response to estrogen
response to ethanol
negative regulation of blood pressure
negative regulation of heart contraction
positive regulation of transcription, DNA-templated
negative regulation of vasoconstriction
positive regulation of vasodilation
positive regulation of transcription from RNA polymerase II promoter
negative regulation of insulin secretion
behavioral response to cocaine
negative regulation of hydrolase activity
negative regulation of cytosolic calcium ion concentration
negative regulation of serotonin uptake
negative regulation of calcium ion transport
regulation of sensory perception of pain
oxidation-reduction process
cellular response to mechanical stimulus
cellular response to growth factor stimulus
response to nitric oxide
cellular response to epinephrine stimulus
positive regulation of the force of heart contraction
positive regulation of long-term synaptic potentiation
positive regulation of adrenergic receptor signaling pathway involved in heart process
positive regulation of neuron death
positive regulation of sodium ion transmembrane transport
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