Proteintech

p115/USO1 Polyclonal Antibody

p115/USO1 Antibody in Immunocytochemistry (ICC/IF)

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p115/USO1 Antibody (13509-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using p115, USO1 antibody (Product # 13509-1-AP) at dilution of 1:1,000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

p115/USO1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

p115/USO1 Antibody in Immunoprecipitation (IP)

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Product Details

13509-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 13 publications 13 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

View 12 publications 12 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

p115, USO1 Fusion Protein Ag4431 (660-961 aa encoded by BC032654)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: EQDLQLEEL RQQVSTLKCQ NEQLQTAVTQ QVSQIQQHKD QYNLLKIQLG KDNQHQGSYS EGAQMNGIQP EEIGRLREEI EELKRNQELL QSQLTEKDSM IENMKSSQTS GTNEQSSAIV SARDSEQVAE LKQELATLKS QLNSQSVEIT KLQTEKQELL QKTEAFAKSV EVQGETETII ATKTTDVEGR LSALLQETKE LKNEIKALSE ERTAIKEQLD SSNSTIAILQ TEKDKLELEI TDSKKEQDDL LVLLADQDQK ILSLKNKLKD LGHPVEEEDE LESGDQEDED DESEDPGKDL DHI (660-961 aa encoded by BC032654)

Target Information

The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Transport from the ER to the cis/medial Golgi compartments requires the action of this gene product, GM130 and giantin in a sequential manner.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A pref-1-controlled non-inflammatory mechanism of insulin resistance.

iScience

Huang Y,Cui D,Chen L,Tong H,Wu H,Muller GK,Qi Y,Wang S,Xu J,Gao X,Fifield KE,Wang L,Xia Z,Vanderluit JL,Liu S,Leng L,Sun G,McGuire J,Young LH,Bucala R,Qi D

Fri Jun 16 00:00:00 EDT 2023

A pref-1-controlled non-inflammatory mechanism of insulin resistance.

iScience

Huang Y,Cui D,Chen L,Tong H,Wu H,Muller GK,Qi Y,Wang S,Xu J,Gao X,Fifield KE,Wang L,Xia Z,Vanderluit JL,Liu S,Leng L,Sun G,McGuire J,Young LH,Bucala R,Qi D

Fri Jun 16 00:00:00 EDT 2023

Golgi organization is a determinant of stem cell function in the small intestine.

bioRxiv : the preprint server for biology

Scharaw S,Sola-Carvajal A,Belevich I,Webb AT,Das S,Andersson S,Pentinmikko N,Villablanca EJ,Goldenring JR,Jokitalo E,Coffey RJ,Katajisto P

Fri Mar 24 00:00:00 EDT 2023

The Immune-Specific E3 Ubiquitin Ligase MARCH1 Is Upregulated during Human Cytomegalovirus Infection to Regulate Iron Levels.

Journal of virology

Martin M,Sandhu P,Kumar R,Buchkovich NJ

Wed Mar 23 00:00:00 EDT 2022

An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress.

Molecular cell

Nüchel J,Tauber M,Nolte JL,Mörgelin M,Türk C,Eckes B,Demetriades C,Plomann M

Thu Aug 19 00:00:00 EDT 2021

An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress.

Molecular cell

Nüchel J,Tauber M,Nolte JL,Mörgelin M,Türk C,Eckes B,Demetriades C,Plomann M

Thu Aug 19 00:00:00 EDT 2021

Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the Golgi structure.

The Journal of cell biology

Zhang Y,Seemann J

Mon Jan 04 00:00:00 EST 2021

Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the Golgi structure.

The Journal of cell biology

Zhang Y,Seemann J

Mon Jan 04 00:00:00 EST 2021

Sec22b Regulates Inflammatory Responses by Controlling the Nuclear Translocation of NF-κB and the Secretion of Inflammatory Mediators.

Journal of immunology (Baltimore, Md. : 1950)

Arango Duque G,Dion R,Matte C,Fabié A,Descoteaux J,Stäger S,Descoteaux A

Mon Nov 01 00:00:00 EDT 2021

Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.

Communications biology

Yue X,Tiwari N,Zhu L,Ngo HDT,Lim JM,Gim B,Jing S,Wang Y,Qian Y,Lee I

Tue Dec 07 00:00:00 EST 2021

Focused CRISPR-Cas9 genetic screening reveals USO1 as a vulnerability in B-cell acute lymphoblastic leukemia.

Scientific reports

Jaiswal AK,Truong H,Tran TM,Lin TL,Casero D,Alberti MO,Rao DS

Wed Jun 23 00:00:00 EDT 2021

Defining the proteolytic landscape during enterovirus infection.

PLoS pathogens

Saeed M,Kapell S,Hertz NT,Wu X,Bell K,Ashbrook AW,Mark MT,Zebroski HA,Neal ML,Flodström-Tullberg M,MacDonald MR,Aitchison JD,Molina H,Rice CM

Tue Sep 01 00:00:00 EDT 2020

Herpes Simplex Virus 1-Induced Blood-Brain Barrier Damage Involves Apoptosis Associated With GM130-Mediated Golgi Stress.

Frontiers in molecular neuroscience

He Q,Liu H,Huang C,Wang R,Luo M,Lu W

Mon Sep 28 00:00:00 EDT 2020

Herpes Simplex Virus 1-Induced Blood-Brain Barrier Damage Involves Apoptosis Associated With GM130-Mediated Golgi Stress.

Frontiers in molecular neuroscience

He Q,Liu H,Huang C,Wang R,Luo M,Lu W

Mon Sep 28 00:00:00 EDT 2020

Human Cytomegalovirus Decreases Major Histocompatibility Complex Class II by Regulating Class II Transactivator Transcript Levels in a Myeloid Cell Line.

Journal of virology

Sandhu PK,Buchkovich NJ

Tue Mar 17 00:00:00 EDT 2020

GM130 and p115 play a key role in the organisation of the early secretory pathway during skeletal muscle differentiation.

Journal of cell science

Giacomello E,Ronchi P,Pepperkok R

Wed Jan 23 00:00:00 EST 2019

GM130 and p115 play a key role in the organisation of the early secretory pathway during skeletal muscle differentiation.

Journal of cell science

Giacomello E,Ronchi P,Pepperkok R

Wed Jan 23 00:00:00 EST 2019

GM130 and p115 play a key role in the organisation of the early secretory pathway during skeletal muscle differentiation.

Journal of cell science

Giacomello E,Ronchi P,Pepperkok R

Wed Jan 23 00:00:00 EST 2019

GM130 and p115 play a key role in the organisation of the early secretory pathway during skeletal muscle differentiation.

Journal of cell science

Giacomello E,Ronchi P,Pepperkok R

Wed Jan 23 00:00:00 EST 2019

Inhibition of diverse opportunistic viruses by structurally optimized retrograde trafficking inhibitors.

Bioorganic & medicinal chemistry

Desai D,Lauver M,Ostman A,Cruz L,Ferguson K,Jin G,Roper B,Brosius D,Lukacher A,Amin S,Buchkovich N

Wed May 01 00:00:00 EDT 2019

cTAGE5 deletion in pancreatic β cells impairs proinsulin trafficking and insulin biogenesis in mice.

The Journal of cell biology

Fan J,Wang Y,Liu L,Zhang H,Zhang F,Shi L,Yu M,Gao F,Xu Z

Mon Dec 04 00:00:00 EST 2017

cTAGE5 deletion in pancreatic β cells impairs proinsulin trafficking and insulin biogenesis in mice.

The Journal of cell biology

Fan J,Wang Y,Liu L,Zhang H,Zhang F,Shi L,Yu M,Gao F,Xu Z

Mon Dec 04 00:00:00 EST 2017

Small GTPase Rab1B is associated with ATG9A vesicles and regulates autophagosome formation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Kakuta S,Yamaguchi J,Suzuki C,Sasaki M,Kazuno S,Uchiyama Y

Fri Sep 01 00:00:00 EDT 2017

Small GTPase Rab1B is associated with ATG9A vesicles and regulates autophagosome formation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Kakuta S,Yamaguchi J,Suzuki C,Sasaki M,Kazuno S,Uchiyama Y

Fri Sep 01 00:00:00 EDT 2017

The Arf GEF GBF1 and Arf4 synergize with the sensory receptor cargo, rhodopsin, to regulate ciliary membrane trafficking.

Journal of cell science

Wang J,Fresquez T,Kandachar V,Deretic D

Fri Dec 01 00:00:00 EST 2017

The Arf GEF GBF1 and Arf4 synergize with the sensory receptor cargo, rhodopsin, to regulate ciliary membrane trafficking.

Journal of cell science

Wang J,Fresquez T,Kandachar V,Deretic D

Fri Dec 01 00:00:00 EST 2017

Globozoospermia and lack of acrosome formation in GM130-deficient mice.

Cell death & disease

Han F,Liu C,Zhang L,Chen M,Zhou Y,Qin Y,Wang Y,Chen M,Duo S,Cui X,Bao S,Gao F

Thu Jan 05 00:00:00 EST 2017

Globozoospermia and lack of acrosome formation in GM130-deficient mice.

Cell death & disease

Han F,Liu C,Zhang L,Chen M,Zhou Y,Qin Y,Wang Y,Chen M,Duo S,Cui X,Bao S,Gao F

Thu Jan 05 00:00:00 EST 2017

AmotL2 disrupts apical-basal cell polarity and promotes tumour invasion.

Nature communications

Mojallal M,Zheng Y,Hultin S,Audebert S,van Harn T,Johnsson P,Lenander C,Fritz N,Mieth C,Corcoran M,Lembo F,Hallström M,Hartman J,Mazure NM,Weide T,Grandér D,Borg JP,Uhlén P,Holmgren L

Fri Aug 01 00:00:00 EDT 2014

AmotL2 disrupts apical-basal cell polarity and promotes tumour invasion.

Nature communications

Mojallal M,Zheng Y,Hultin S,Audebert S,van Harn T,Johnsson P,Lenander C,Fritz N,Mieth C,Corcoran M,Lembo F,Hallström M,Hartman J,Mazure NM,Weide T,Grandér D,Borg JP,Uhlén P,Holmgren L

Fri Aug 01 00:00:00 EDT 2014

Golgi disruption and early embryonic lethality in mice lacking USO1.

PloS one

Kim S,Hill A,Warman ML,Smits P

Mon May 13 00:00:00 EDT 2013

Golgi disruption and early embryonic lethality in mice lacking USO1.

PloS one

Kim S,Hill A,Warman ML,Smits P

Mon May 13 00:00:00 EDT 2013

Golgi disruption and early embryonic lethality in mice lacking USO1.

PloS one

Kim S,Hill A,Warman ML,Smits P

Mon May 13 00:00:00 EDT 2013

Golgi disruption and early embryonic lethality in mice lacking USO1.

PloS one

Kim S,Hill A,Warman ML,Smits P

Mon May 13 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: General vesicular transport factor p115; Protein USO1 homolog; TAP; transcytosis associated protein; transcytosis associated protein p115; Transcytosis-associated protein; USO1 homolog, vesicle docking protein; USO1 vesicle docking protein homolog; VDP p115; vesicle docking protein p115; vesicle docking protein, 115 kDa; Vesicle-docking protein

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Gene Aliases: 115kDa; P115; TAP; USO1; VDP

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UniProt ID: (Human) O60763, (Mouse) Q9Z1Z0, (Rat) P41542

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Entrez Gene ID: (Human) 8615, (Mouse) 56041, (Rat) 56042

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Function(s)

protein binding protein transporter activity poly(A) RNA binding cadherin binding involved in cell-cell adhesion membrane traffic protein

Process(es)

intracellular protein transport ER to Golgi vesicle-mediated transport transcytosis COPII vesicle coating Golgi vesicle docking vesicle fusion with Golgi apparatus membrane fusion cell-cell adhesion transport protein transport vesicle-mediated transport Golgi vesicle transport intra-Golgi vesicle-mediated transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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p115/USO1 Polyclonal Antibody

p115/USO1 Antibody (13509-1-AP) in ICC/IF

Product Details

13509-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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