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p130 Antibody (600-401-MP1) in WB
Western Blot of Rabbit Anti-p130 Antibody. Lane 1: MOLT-4 Lysate (p/n W09-001-GK2) (1µg) [+]. Lane 2: MOLT-4 Lysate (p/n W09-001-GK2) (10µg) [+]. Lane 3: Human Eye Lysate (1µg) [-]. Lane 4: Human Eye Lysate (10µg) [-]. Lane 5: Opal Pre-stained Molecular Weight Marker (p/n MB-210-0500). Primary Antibody: Anti-p130 at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:10,000 for 1hr at RT. Blocking Buffer: Universal BlockOut (p/n MB-073). Predicted MW: ~128.4kDa. Observed MW: ~125kDa. Exposur... View More
Product Details
600-401-MP1
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-p130 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal portion of human Retinoblastoma-like protein 2 conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugate
Form
Liquid
Concentration
0.92 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against human Retinoblastoma-like protein 2. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to rat,human, and mouse.
Target Information
RBL2 (also known as p130) or Retinoblastoma-like protein 2 is a key regulator of entry into cell division. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and (in particular) that of constitutive heterochromatin by stabilizing histone methylation. RBL2 recruits and targets histone methyltransferases KMT5B and KMT5C leading to epigenetic transcriptional repression. It controls histone H4 'Lys20' trimethylation. It probably also acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. RBL2 serves as a potent inhibitor of E2F-mediated trans-activation and associates preferentially with E2F5.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 130 kDa retinoblastoma-associated protein; FLJ26459; p130; PRB2; rb2 p130; RBR-2; retinoblastoma-like 2; Retinoblastoma-like protein 2; Retinoblastoma-related protein 2
Gene Aliases: P130; RB2; RBL2
UniProt ID: (Human) Q08999
Entrez Gene ID: (Human) 5934
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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