Invitrogen

p16INK4a Monoclonal Antibody (1E12E10)

7 Published Figures

14 References

View all (154) p16INK4a antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (7)

p16INK4a Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 9

p16INK4a Antibody (MA5-17142) in IHC (P)

Immunohistochemical analysis of paraffin-embedded rat liver tissue (A), human brain tumor (B), breast cancer (C), esophageal epithelium tissue (D), mouse brain tissue (E) and stomach tisue (F) using P16 monoclonal antibody (Product # MA5-17142) followed with DAB staining.

p16INK4a Antibody in Immunohistochemistry (IHC)

p16INK4a Antibody in Immunocytochemistry (ICC/IF)

p16INK4a Antibody in Immunohistochemistry (Frozen) (IHC (F))

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Product Details

MA5-17142

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

1:10,000

in situ PLA (PLA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1E12E10

Immunogen

Purified recombinant fragment of P16 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2538613

Product Specific Information

MA5-17142 targets P16 in indirect ELISA, IHC and WB applications and shows reactivity with Human, Mouse, and Rat samples.

The MA5-17142 immunogen is purified recombinant fragment of P16 expressed in E. Coli.

Target Information

This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SATB2, coordinated with CUX1, regulates IL-1β-induced senescence-like phenotype in endothelial cells by fine-tuning the atherosclerosis-associated p16^INK4a expression.

Aging cell

Wu T,Wu Y,Jiang D,Sun W,Zou M,Vasamsetti SB,Dutta P,Leers SA,Di W,Li G

MA5-17142 was used in Immunocytochemistry and Western Blotting to demonstrate that IL-1β, a senescence-associated secretory phenotype (SASP) gene itself and a biomarker for atherosclerosis, induces cellular senescence also by upregulating CUX1 and/or downregulating SATB2 in human endothelial cells..

Wed Feb 01 00:00:00 EST 2023

SATB2, coordinated with CUX1, regulates IL-1β-induced senescence-like phenotype in endothelial cells by fine-tuning the atherosclerosis-associated p16^INK4a expression.

Aging cell

Wu T,Wu Y,Jiang D,Sun W,Zou M,Vasamsetti SB,Dutta P,Leers SA,Di W,Li G

Wed Feb 01 00:00:00 EST 2023

Senolytic therapy is neuroprotective and improves functional outcome long-term after traumatic brain injury in mice.

Frontiers in neuroscience

Wang J,Lu Y,Carr C,Dhandapani KM,Brann DW

MA5-17142 was used in Immunohistochemistry-immunofluorescence to indicate there is robust and widespread induction of senescent cells in the brain long-term after TBI, and that senolytic drug treatment begun 1-month after TBI can efficiently ablate the senescent cells, reduce expression of proinflammatory SASP factors, reduce neurodegeneration, and rescue defects in reference memory, recognition memory, and depressive behavior.

Wed Dec 06 00:00:00 EST 2023

Senolytic therapy is neuroprotective and improves functional outcome long-term after traumatic brain injury in mice.

Frontiers in neuroscience

Wang J,Lu Y,Carr C,Dhandapani KM,Brann DW

Wed Dec 06 00:00:00 EST 2023

iPSC-sEVs alleviate microglia senescence to protect against ischemic stroke in aged mice.

Materials today. Bio

Niu X,Xia Y,Luo L,Chen Y,Yuan J,Zhang J,Zheng X,Li Q,Deng Z,Wang Y

MA5-17142 was used in Western Blotting to show that treatment with iPSC-sEVs significantly alleviated microglia senescence as indicated by the decreased senescence-associated proteins including P16, P21, P53, and γ-H2AX as well as the activity of SA-β-gal, and inhibited pro-inflammatory activation of microglia both in vivo and in vitro.

Sat Apr 01 00:00:00 EDT 2023

iPSC-sEVs alleviate microglia senescence to protect against ischemic stroke in aged mice.

Materials today. Bio

Niu X,Xia Y,Luo L,Chen Y,Yuan J,Zhang J,Zheng X,Li Q,Deng Z,Wang Y

Sat Apr 01 00:00:00 EDT 2023

Senescence atlas reveals an aged-like inflamed niche that blunts muscle regeneration.

Nature

Moiseeva V,Cisneros A,Sica V,Deryagin O,Lai Y,Jung S,Andrés E,An J,Segalés J,Ortet L,Lukesova V,Volpe G,Benguria A,Dopazo A,Benitah SA,Urano Y,Del Sol A,Esteban MA,Ohkawa Y,Serrano AL,Perdiguero E,Muñoz-Cánoves P

MA5-17142 was used in Flow cytometry/Cell sorting to provide a technique for isolating in vivo senescent cells, define a senescence blueprint for muscle, and uncover unproductive functional interactions between senescent cells and stem cells in regenerative niches that can be overcome.

Sun Jan 01 00:00:00 EST 2023

Accelerated Senescence and Apoptosis in the Rat Liver during the Progression of Diabetic Complications.

The Malaysian journal of medical sciences : MJMS

Yuniartha R,Arfian N,Setyaningsih WAW,Kencana SMS,Sari DCR

MA5-17142 was used in ELISA, Immunohistochemistry (Paraffin), Western Blot to determine the pathological complications of Chronic hyperglycaemia in the rat liver.

Thu Dec 01 00:00:00 EST 2022

Accelerated Senescence and Apoptosis in the Rat Liver during the Progression of Diabetic Complications.

The Malaysian journal of medical sciences : MJMS

Yuniartha R,Arfian N,Setyaningsih WAW,Kencana SMS,Sari DCR

MA5-17142 was used in ELISA, Immunohistochemistry (Paraffin), Western Blot to determine the pathological complications of Chronic hyperglycaemia in the rat liver.

Thu Dec 01 00:00:00 EST 2022

Attenuation of intrinsic ageing of the skin via elimination of senescent dermal fibroblasts with senolytic drugs.

Journal of the European Academy of Dermatology and Venereology : JEADV

Kim H,Jang J,Song MJ,Kim G,Park CH,Lee DH,Lee SH,Chung JH

MA5-17142 was used in ELISA, Immunohistochemistry (Paraffin), Western Blot to indicate that selective clearance of senescent skin cells can attenuate and improve skin ageing phenotypes and that senolytic drugs may be of potential use as new therapeutic agents for treating ageing of the skin.

Fri Jul 01 00:00:00 EDT 2022

Post-GWAS functional analysis identifies CUX1 as a regulator of p16^INK4a and cellular senescence.

Nature aging

Jiang D,Sun W,Wu T,Zou M,Vasamsetti SB,Zhang X,Zhao Y,Phillippi JA,Sawalha AH,Tavakoli S,Dutta P,Florentin J,Chan SY,Tollison TS,Cui J,Huntress I,Peng X,Finkel T,Li G

MA5-17142 was used in Immunocytochemistry to identify CUX1 as a regulator of p16INK4a-dependent endothelial senescence and a potential therapeutic target for atherosclerosis and other age-related diseases.

Tue Feb 01 00:00:00 EST 2022

Hypoxia-inducible factor-2α mediates senescence-associated intrinsic mechanisms of age-related bone loss.

Experimental & molecular medicine

Lee SY,Park KH,Lee G,Kim SJ,Song WH,Kwon SH,Koh JT,Huh YH,Ryu JH

Published figure using p16INK4a monoclonal antibody (Product # MA5-17142) in Immunohistochemistry

Thu Apr 01 00:00:00 EDT 2021

Molecular markers of risk of subsequent invasive breast cancer in women with ductal carcinoma in situ: protocol for a population-based cohort study.

BMJ open

Rohan TE,Ginsberg M,Wang Y,Couch FJ,Feigelson HS,Greenlee RT,Honda S,Stark A,Chitale D,Wang T,Xue X,Oktay MH,Sparano JA,Loudig O

MA5-17142 was used in Immunohistochemistry to facilitate detection of patients with ductal carcinoma in situ at risk of invasive breast cancer development.

Tue Oct 26 00:00:00 EDT 2021

Cdkn2a Loss in a Model of Neurofibroma Demonstrates Stepwise Tumor Progression to Atypical Neurofibroma and MPNST.

Cancer research

Chaney KE,Perrino MR,Kershner LJ,Patel AV,Wu J,Choi K,Rizvi TA,Dombi E,Szabo S,Largaespada DA,Ratner N

MA5-17142 was used in Western Blotting to propose a new mouse model which recapitulates the stepwise progression of neurofibromatosis type 1 tumours to help define effective treatments that halt tumour growth and progression.

Sun Nov 01 00:00:00 EDT 2020

TGF-β1/IL-11/MEK/ERK signaling mediates senescence-associated pulmonary fibrosis in a stress-induced premature senescence model of Bmi-1 deficiency.

Experimental & molecular medicine

Chen H,Chen H,Liang J,Gu X,Zhou J,Xie C,Lv X,Wang R,Li Q,Mao Z,Sun H,Zuo G,Miao D,Jin J

MA5-17142 was used in Proximity Ligation Assay (PLA) to study whether TGF-β1/IL-11/MEK/ERK (TIME) signaling mediates senescence-associated pulmonary fibrosis (SAPF) in Bmi-1-deficient (Bmi-1-/-) mice and determines the major downstream mediator of Bmi-1 and crosstalk between p16INK4a and reactive oxygen species that regulates SAPF.

Wed Jan 01 00:00:00 EST 2020

Chronic Intermittent Hypoxia Triggers a Senescence-like Phenotype in Human White Preadipocytes.

Scientific reports

Polonis K,Becari C,Chahal CAA,Zhang Y,Allen AM,Kellogg TA,Somers VK,Singh P

MA5-17142 was used in Immunocytochemistry-immunoflourescence to demonstrate that chronic intermittent hypoxia (IH) is associated with an increased generation of mitochondrial reactive oxygen species along with increased prevalence of cells with nuclear localization of γH2AX & p16.

Wed Apr 22 00:00:00 EDT 2020

Chronic Intermittent Hypoxia Triggers a Senescence-like Phenotype in Human White Preadipocytes.

Scientific reports

Polonis K,Becari C,Chahal CAA,Zhang Y,Allen AM,Kellogg TA,Somers VK,Singh P

Wed Apr 22 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: Alternative reading frame; ARF; CDK4 inhibitor p16 INK4; CDK4 inhibitor p16-INK4; CDK4I; cdkn2a; cdkn2a {ECO:0000312|EMBL:AAL76336.1, ECO:0000312|RGD:2323}; cdkn2a {ECO:0000312|EMBL:AAL76338.1, ECO:0000312|RGD:2323}; cell cycle inhibitor; cell cycle negative regulator beta; cell cycle regulator; Cyclin dependent kinase 4 inhibitor A; Cyclin dependent kinase inhibitor 2A (p16, inhibits CDK4); Cyclin-dependent kinase 4 inhibitor A; Cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2a p16Ink4a; cyclin-dependent kinase inhibitor 2a p19Arf; cyclin-dependent kinase inhibitor 2A, isoform 1; cyclin-dependent kinase inhibitor 2A, isoform 2; cyclin-dependent kinase inhibitor 2A, isoform 3; cyclin-dependent kinase inhibitor 2A, isoforms 1/2; cyclin-dependent kinase inhibitor protein; HGNC:1787; inhibits CDK4; isoforms 1/2/3; Melanoma p16 inhibits CDK4; mitochondrial smARF; MTS-1; Multiple tumor suppressor 1; P14 P16; p14ARF; p16; p16-INK4; p16-INK4a; P19 TP16; p19ARF; Tumor suppressor ARF

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Gene Aliases: ARF; ARF-INK4a; CDK4I; CDKN2; CDKN2A; CMM2; INK4; INK4A; INK4a-ARF; Ink4a/Arf; MLM; MTS-1; MTS1; P14; P14ARF; P16; p16(INK4a); P16-INK4A; p16Cdkn2a; P16INK4; P16INK4A; P19; p19; P19ARF; Pctr1; TP16

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UniProt ID: (Human) Q8N726, (Mouse) Q64364, (Rat) Q9R0Z3

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Entrez Gene ID: (Human) 1029, (Mouse) 12578, (Rat) 25163

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Function(s)

cyclin-dependent protein serine/threonine kinase inhibitor activity protein binding protein kinase binding poly(A) RNA binding NF-kappaB binding p53 binding DNA binding transcription factor activity, sequence-specific DNA binding transcription factor binding protein N-terminus binding ubiquitin-protein transferase inhibitor activity MDM2/MDM4 family protein binding

Process(es)

Performance Guarantee

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p16INK4a Monoclonal Antibody (1E12E10)

p16INK4a Antibody (MA5-17142) in IHC (P)

Product Details

MA5-17142

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

in situ PLA (PLA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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