Proteintech

p21 Polyclonal Antibody

p21 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 2

p21 Antibody (28248-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 28248-1-AP (p21 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red).

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Product Details

28248-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:800

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:200

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Cdkn1a Fusion Protein Ag28394

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

467 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

Protein p21 is a tumor suppressor protein which is an inhibitor of Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53. P21 is important in the response of cells to genotoxic stress and a major transcriptional target of p53 protein. The occurrence of p21 in the nucleus executes binding and inhibition activity of cyclin dependent kinases Cdk1 and Cdk2, and blocks the transition from G1 phase into S phase or from G2 phase into mitosis after DNA damage, which enables the repair of damaged DNA. In the cytoplasm, p21 protein has an anti-apoptotic effect. P21 is able to bind to and inhibit caspase 3, as well as the apoptotic kinases ASK1 and JNK. P21 exhibits a dual function in carcinogenesis, and acts as a tumor suppressor, prevents apoptosis, and acts as an oncogene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

METTL3 alleviates D-gal-induced renal tubular epithelial cellular senescence via promoting miR-181a maturation.

Mechanisms of ageing and development

Zhang Y,Ni X,Wei L,Yu Y,Zhu B,Bai Y,Pei X,Gao F,Guo L,Yong Z,Zhao W

Wed Mar 01 00:00:00 EST 2023

METTL3 alleviates D-gal-induced renal tubular epithelial cellular senescence via promoting miR-181a maturation.

Mechanisms of ageing and development

Zhang Y,Ni X,Wei L,Yu Y,Zhu B,Bai Y,Pei X,Gao F,Guo L,Yong Z,Zhao W

Wed Mar 01 00:00:00 EST 2023

METTL3 alleviates D-gal-induced renal tubular epithelial cellular senescence via promoting miR-181a maturation.

Mechanisms of ageing and development

Zhang Y,Ni X,Wei L,Yu Y,Zhu B,Bai Y,Pei X,Gao F,Guo L,Yong Z,Zhao W

Wed Mar 01 00:00:00 EST 2023

METTL3 alleviates D-gal-induced renal tubular epithelial cellular senescence via promoting miR-181a maturation.

Mechanisms of ageing and development

Zhang Y,Ni X,Wei L,Yu Y,Zhu B,Bai Y,Pei X,Gao F,Guo L,Yong Z,Zhao W

Wed Mar 01 00:00:00 EST 2023

Upregulation of TMEM40 is associated with the malignant behavior and promotes tumor progression in cervical cancer.

Discover. Oncology

Zhang ZF,Liu F,Zhang HR,Liu B,Zheng SQ,Ye WQ,Ding JN,Zhou ZJ,Luo HX,Wu F,Guo XM,Zhou JY,Guo YH

Thu Apr 13 00:00:00 EDT 2023

Chito-oligosaccharides and macrophages have synergistic effects on improving ovarian stem cells function by regulating inflammatory factors.

Journal of ovarian research

Zheng K,Hong W,Ye H,Zhou Z,Ling S,Li Y,Dai Y,Zhong Z,Yang Z,Zheng Y

Fri Apr 14 00:00:00 EDT 2023

Intestinal transit amplifying cells require METTL3 for growth factor signaling, KRAS expression, and cell survival.

bioRxiv : the preprint server for biology

Danan CH,Naughton KE,Hayer KE,Vellappan S,McMillan EA,Zhou Y,Matsuda R,Nettleford SK,Katada K,Parham LR,Ma X,Chowdhury A,Wilkins BJ,Shah P,Weitzman MD,Hamilton KE

Fri Apr 21 00:00:00 EDT 2023

Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice.

Redox biology

Zhao B,Yu J,Luo Y,Xie M,Qu C,Shi Q,Wang X,Zhao X,Kong L,Zhao Y,Guo Y

Sat Jul 01 00:00:00 EDT 2023

m6A contributes to a pro-survival state in GC-2 cells by facilitating DNA damage repair: Novel perspectives on the mechanism underlying DEHP genotoxicity in male germ cells.

The Science of the total environment

Fu H,Zhu X,Di Q,Sun J,Jiang Q,Xu Q

Fri Feb 10 00:00:00 EST 2023

Decrease in the expression of muscle-specific miRNAs, miR-133a and miR-1, in myoblasts with replicative senescence.

PloS one

Shintani-Ishida K,Tsurumi R,Ikegaya H

Wed Aug 09 00:00:00 EDT 2023

Immunity and reproduction protective effects of Chitosan Oligosaccharides in Cyclophosphamide/Busulfan-induced premature ovarian failure model mice.

Frontiers in immunology

Li X,Ye H,Su T,Hu C,Huang Y,Fu X,Zhong Z,Du X,Zheng Y

Mon May 29 00:00:00 EDT 2023

Rejuvenation of tendon stem/progenitor cells for functional tendon regeneration through platelet-derived exosomes loaded with recombinant Yap1.

Acta biomaterialia

Lu J,Yang X,He C,Chen Y,Li C,Li S,Chen Y,Wu Y,Xiang Z,Kang J,Jiang G,Wang C,Diarra MD,He R,Feng G,Yan R

Sat Apr 15 00:00:00 EDT 2023

Exploring the molecular mechanisms underlie the endoplasmic reticulum stress-mediated methylmercury-induced neuronal developmental damage.

Ecotoxicology and environmental safety

Pan J,Li X,Liu H,Wang C,Xu S,Xu B,Deng Y,Yang T,Liu W

Sat Oct 15 00:00:00 EDT 2022

Ganoderic acid D prevents oxidative stress-induced senescence by targeting 14-3-3ε to activate CaM/CaMKII/NRF2 signaling pathway in mesenchymal stem cells.

Aging cell

Yuan H,Xu Y,Luo Y,Zhang JR,Zhu XX,Xiao JH

Thu Sep 01 00:00:00 EDT 2022

Revealing the Therapeutic Targets and Mechanism of Ginsenoside Rg1 for Liver Damage Related to Anti-Oxidative Stress Using Proteomic Analysis.

International journal of molecular sciences

Hou J,Ma R,Zhu S,Wang Y

Fri Sep 02 00:00:00 EDT 2022

6-Shogaol Inhibits Oxidative Stress-Induced Rat Vascular Smooth Muscle Cell Apoptosis by Regulating OXR1-p53 Axis.

Frontiers in molecular biosciences

Liu J,Li B,Li W,Pan T,Diao Y,Wang F

Thu Nov 02 00:00:00 EDT 2023

KLF6 facilitates differentiation of odontoblasts through modulating the expression of P21 in vitro.

International journal of oral science

Chen Z,Wu W,Zheng C,Lan Y,Xie H,Xie Z

Thu Apr 14 00:00:00 EDT 2022

KLF6 facilitates differentiation of odontoblasts through modulating the expression of P21 in vitro.

International journal of oral science

Chen Z,Wu W,Zheng C,Lan Y,Xie H,Xie Z

Thu Apr 14 00:00:00 EDT 2022

KLF6 facilitates differentiation of odontoblasts through modulating the expression of P21 in vitro.

International journal of oral science

Chen Z,Wu W,Zheng C,Lan Y,Xie H,Xie Z

Thu Apr 14 00:00:00 EDT 2022

KLF6 facilitates differentiation of odontoblasts through modulating the expression of P21 in vitro.

International journal of oral science

Chen Z,Wu W,Zheng C,Lan Y,Xie H,Xie Z

Thu Apr 14 00:00:00 EDT 2022

Endoderm development requires centrioles to restrain p53-mediated apoptosis in the absence of ERK activity.

Developmental cell

Xie C,Abrams SR,Herranz-Pérez V,García-Verdugo JM,Reiter JF

Mon Dec 20 00:00:00 EST 2021

Sodium hydrosulfide alleviates dexamethasone-induced cell senescence and dysfunction through targeting the miR-22/sirt1 pathway in osteoblastic MC3T3-E1 cells.

Experimental and therapeutic medicine

Li P,Mao WW,Zhang S,Zhang L,Chen ZR,Lu ZD

Mon Mar 01 00:00:00 EST 2021

NRSF deficiency leads to abnormal postnatal development of dentate gyrus and impairment of progenitors in subgranular zone of hippocampus.

Hippocampus

Wang YC,Liu P,Yue LY,Huang F,Xu YX,Zhu CQ

Wed Sep 01 00:00:00 EDT 2021

NRSF deficiency leads to abnormal postnatal development of dentate gyrus and impairment of progenitors in subgranular zone of hippocampus.

Hippocampus

Wang YC,Liu P,Yue LY,Huang F,Xu YX,Zhu CQ

Wed Sep 01 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: Activating Fragment 1; CDK-interacting protein 1; CDNK1A; Cip1); Cyclin-dependent kinase inhibitor 1; Cyclin-dependent kinase inhibitor 1A (p21; cyclin-dependent kinase inhibitor 1A (P21); cyclin-dependent kinase inhibitor 1A (p21, Cip1); cyclin-dependent kinase inhibitor 1A variant 1; cyclin-dependent kinase inhibitor 1A variant 2; Melanoma differentiation-associated protein; OTTHUMP00000016298; p21; p21; p21Cip1/Waf1; SLC12A9; Wild type p53 activated fragment 1 (WAF1)

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Gene Aliases: CAP20; CDKI; Cdkn1; Cdkn1a; CIP1; mda6; P21; p21Cip1; p21WAF; SDI1; UV96; Waf1

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UniProt ID: (Mouse) P39689

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Entrez Gene ID: (Mouse) 12575, (Rat) 114851

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Function(s)

protein kinase inhibitor activity cyclin-dependent protein serine/threonine kinase inhibitor activity protein binding cyclin-dependent protein kinase activating kinase activity cyclin binding ubiquitin protein ligase binding protein complex binding metal ion binding kinase inhibitor

Process(es)

regulation of cyclin-dependent protein serine/threonine kinase activity G1/S transition of mitotic cell cycle G2/M transition of mitotic cell cycle protein phosphorylation cellular response to DNA damage stimulus DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest cell cycle cell cycle arrest regulation of mitotic cell cycle negative regulation of cell proliferation response to UV negative regulation of gene expression negative regulation of cell growth positive regulation of B cell proliferation cellular response to extracellular stimulus regulation of protein import into nucleus, translocation negative regulation of phosphorylation intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator negative regulation of apoptotic process positive regulation of programmed cell death negative regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of protein kinase activity positive regulation of fibroblast proliferation regulation of cell cycle cellular response to ionizing radiation cellular response to UV-B mitotic cell cycle arrest signal transduction by p53 class mediator cellular senescence negative regulation of G1/S transition of mitotic cell cycle regulation of DNA biosynthetic process positive regulation of reactive oxygen species metabolic process Ras protein signal transduction response to toxic substance response to organic substance response to X-ray response to organonitrogen compound response to organic cyclic compound organ regeneration cellular response to heat response to drug response to arsenic-containing substance response to glucocorticoid response to corticosterone response to hyperoxia intestinal epithelial cell maturation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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p21 Polyclonal Antibody

p21 Antibody (28248-1-AP) in ICC/IF

Product Details

28248-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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