Invitrogen

p21 Monoclonal Antibody (EA10)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

5 Published Figures

21 References

View all (83) p21 antibodies

Datasheet

Protocols

Tech Support

Datasheet

Protocols

Tech Support

p21 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

p21 Antibody (33-7000) in ICC/IF

Immunofluorescent analysis of p21 was done on 70% confluent log phase U2OS cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton™ X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with p21 Mouse Monoclonal Antibody (Product # 33-7000) at 2 µg - 4 µg in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor® 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 m... View More

p21 Antibody in Immunohistochemistry (IHC)

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p21 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF7 cells were transfected with p21 siRNA and loss of signal was observed in Western Blot using anti-p21 Monoclonal Antibody (EA10) (Product # 33-7000). {KD}

Product Details

33-7000

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 9 publications 9 publications

Immunohistochemistry (IHC)

Assay-dependent

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

EA10

Immunogen

Full length recombinant human p21Cip1/WAF1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533135

Target Information

Protein p21 is a tumor suppressor protein which is an inhibitor of Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53. P21 is important in the response of cells to genotoxic stress and a major transcriptional target of p53 protein. The occurrence of p21 in the nucleus executes binding and inhibition activity of cyclin dependent kinases Cdk1 and Cdk2, and blocks the transition from G1 phase into S phase or from G2 phase into mitosis after DNA damage, which enables the repair of damaged DNA. In the cytoplasm, p21 protein has an anti-apoptotic effect. P21 is able to bind to and inhibit caspase 3, as well as the apoptotic kinases ASK1 and JNK. P21 exhibits a dual function in carcinogenesis, and acts as a tumor suppressor, prevents apoptosis, and acts as an oncogene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Increased p53 signaling impairs neural differentiation in HUWE1-promoted intellectual disabilities.

Cell reports. Medicine

Aprigliano R,Aksu ME,Bradamante S,Mihaljevic B,Wang W,Rian K,Montaldo NP,Grooms KM,Fordyce Martin SL,Bordin DL,Bosshard M,Peng Y,Alexov E,Skinner C,Liabakk NB,Sullivan GJ,Bjørås M,Schwartz CE,van Loon B

33-7000 was used in Western Blot to suggest that increased p53 signaling underlies HUWE1-promoted syndromes and impairs XLID JMS neural differentiation.

Tue Apr 20 00:00:00 EDT 2021

Increased p53 signaling impairs neural differentiation in HUWE1-promoted intellectual disabilities.

Cell reports. Medicine

33-7000 was used in Western Blot to suggest that increased p53 signaling underlies HUWE1-promoted syndromes and impairs XLID JMS neural differentiation.

Tue Apr 20 00:00:00 EDT 2021

CCNB1 Expedites the Progression of Cervical Squamous Cell Carcinoma via the Regulation by FOXM1.

OncoTargets and therapy

Li S,Liu N,Piao J,Meng F,Li Y

33-7000 was used in Western Blotting to explore the mechanism underlying CCNB1 regulation on cell cycle progression in cervical squamous cell carcinoma.

Mon Oct 24 00:00:00 EDT 2022

CCNB1 Expedites the Progression of Cervical Squamous Cell Carcinoma via the Regulation by FOXM1.

OncoTargets and therapy

Li S,Liu N,Piao J,Meng F,Li Y

33-7000 was used in Western Blotting to explore the mechanism underlying CCNB1 regulation on cell cycle progression in cervical squamous cell carcinoma.

Mon Oct 24 00:00:00 EDT 2022

Oxidative stress-mediated mitochondrial dysfunction facilitates mesenchymal stem cell senescence in ankylosing spondylitis.

Cell death & disease

Ye G,Xie Z,Zeng H,Wang P,Li J,Zheng G,Wang S,Cao Q,Li M,Liu W,Cen S,Li Z,Wu Y,Ye Z,Shen H

33-7000 was used in Immunohistochemistry (Frozen), Immunocytochemistry to demonstrate that the oxidative serum environment of AS facilitated MSC senescence through inducing mitochondrial dysfunction and excessive ROS production.

Thu Sep 17 00:00:00 EDT 2020

Oxidative stress-mediated mitochondrial dysfunction facilitates mesenchymal stem cell senescence in ankylosing spondylitis.

Cell death & disease

Ye G,Xie Z,Zeng H,Wang P,Li J,Zheng G,Wang S,Cao Q,Li M,Liu W,Cen S,Li Z,Wu Y,Ye Z,Shen H

Thu Sep 17 00:00:00 EDT 2020

Oxidative stress-mediated mitochondrial dysfunction facilitates mesenchymal stem cell senescence in ankylosing spondylitis.

Cell death & disease

Ye G,Xie Z,Zeng H,Wang P,Li J,Zheng G,Wang S,Cao Q,Li M,Liu W,Cen S,Li Z,Wu Y,Ye Z,Shen H

Thu Sep 17 00:00:00 EDT 2020

Generation of Human Fatty Livers Using Custom-Engineered Induced Pluripotent Stem Cells with Modifiable SIRT1 Metabolism.

Cell metabolism

Collin de l'Hortet A,Takeishi K,Guzman-Lepe J,Morita K,Achreja A,Popovic B,Wang Y,Handa K,Mittal A,Meurs N,Zhu Z,Weinberg F,Salomon M,Fox IJ,Deng CX,Nagrath D,Soto-Gutierrez A

33-7000 was used in Immunohistochemistry-immunofluorescence to generate human induced pluripotent stem cells with controllable expression of SIRT1 to model human fatty livers.

Tue Aug 06 00:00:00 EDT 2019

Parthenolide inhibits the proliferation and induces the apoptosis of human uveal melanoma cells.

International journal of ophthalmology

Che ST,Bie L,Li X,Qi H,Yu P,Zuo L

33-7000 was used in Western Blotting to deduce that human uveal melanoma cell proliferation can be inhibited and apoptosis induced by parthenolide through the arrest of G1 phase and regulation of the mitochondrial pathway.

Thu Oct 01 00:00:00 EDT 2020

Pooled screening for antiproliferative inhibitors of protein-protein interactions.

Nature chemical biology

Nim S,Jeon J,Corbi-Verge C,Seo MH,Ivarsson Y,Moffat J,Tarasova N,Kim PM

33-7000 was used in western blot to assess antiproliferative inhibitors of protein-protein interactions by pooled screening

Fri Apr 01 00:00:00 EDT 2016

Senescent human hepatocytes express a unique secretory phenotype and promote macrophage migration.

World journal of gastroenterology

Irvine KM,Skoien R,Bokil NJ,Melino M,Thomas GP,Loo D,Gabrielli B,Hill MM,Sweet MJ,Clouston AD,Powell EE

33-7000 was used in immunocytochemistry to generate a model of stress-induced senescence and study the hepatocyte senescence associated secretory phenotype

Sun Dec 21 00:00:00 EST 2014

MicroRNA-224 negatively regulates p21 expression during late neoplastic progression in inflammatory bowel disease.

Inflammatory bowel diseases

Olaru AV,Yamanaka S,Vazquez C,Mori Y,Cheng Y,Abraham JM,Bayless TM,Harpaz N,Selaru FM,Meltzer SJ

33-7000 was used in western blot to investigate miR dysregulation in colonic specimens from patients with inflammatory bowel disease.

Fri Mar 01 00:00:00 EST 2013

A study of the combination of triamcinolone and 5-fluorouracil in modulating keloid fibroblasts in vitro.

Journal of plastic, reconstructive & aesthetic surgery : JPRAS

Huang L,Cai YJ,Lung I,Leung BC,Burd A

33-7000 was used in western blot to assess the combined treatment with triamcinolone and 5-fluorouracil for the intralesional therapy of keloids.

Sun Sep 01 00:00:00 EDT 2013

Targeting CDH17 suppresses tumor progression in gastric cancer by downregulating Wnt/β-catenin signaling.

PloS one

Qiu HB,Zhang LY,Ren C,Zeng ZL,Wu WJ,Luo HY,Zhou ZW,Xu RH

Published figure using p21 monoclonal antibody (Product # 33-7000) in Western Blot

Mon Sep 30 00:00:00 EDT 2013

Optimization of immunolocalization of cell cycle proteins in human corneal endothelial cells.

Molecular vision

He Z,Campolmi N,Ha Thi BM,Dumollard JM,Peoc'h M,Garraud O,Piselli S,Gain P,Thuret G

Published figure using p21 monoclonal antibody (Product # 33-7000) in Immunofluorescence

Thu Apr 26 00:00:00 EDT 2012

Low-dose 5-fluorouracil induces cell cycle G2 arrest and apoptosis in keloid fibroblasts.

The British journal of dermatology

Huang L,Wong YP,Cai YJ,Lung I,Leung CS,Burd A

33-7000 was used in western blot to discuss the use of low-dose 5-fluorouracil to treat keloid scarring

Wed Dec 01 00:00:00 EST 2010

22-oxa-1,25-dihydroxyvitamin D3 efficiently inhibits tumor growth in inoculated mice and primary histoculture of cholangiocarcinoma.

Cancer

Seubwai W,Wongkham C,Puapairoj A,Okada S,Wongkham S

33-7000 was used in immunocytochemistry and western blot to investigate how 22-oxa-D3 affects the proliferation of cholangiocarcinoma

Wed Dec 01 00:00:00 EST 2010

Clinical and immunohistochemical features associated with a response to bortezomib in patients with multiple myeloma.

Clinical cancer research : an official journal of the American Association for Cancer Research

Dawson MA,Opat SS,Taouk Y,Donovan M,Zammit M,Monaghan K,Horvath N,Roberts AW,Prince HM,Hertzberg M,McLean CA,Spencer A

Thu Jan 15 00:00:00 EST 2009

The abnormalities in the p53/p21WAF1 pathway have a significant role in the pathogenesis and progression of gastrointestinal stromal tumors.

Oncology reports

Chou YP,Lin JW,Wang CC,Chiu YC,Huang CC,Chuah SK,Tai MH,Yi LN,Lee CM,Changchien CS,Hu TH

33-7000 was used in immunohistochemistry - paraffin section to assess the prognostic role and relationship of the p53/p21/PCNA pathway in gastrointestinal stromal tumors

Tue Jan 01 00:00:00 EST 2008

Elevated p21 expression is associated with poor prognosis of rectal stromal tumors after resection.

Journal of surgical oncology

Hu TH,Tai MH,Chuah SK,Chen HH,Lin JW,Huang HY,Chou YP,Yi LN,Kuo CM,Changchien CS

33-7000 was used in immunohistochemistry - paraffin section to determine the prognostic role of p21, p53, PCNA, and Ki-67 in rectal stromal tumors

Fri Aug 01 00:00:00 EDT 2008

Immunohistochemical analysis of non-small cell lung cancer: correlation with clinical parameters and prognosis.

Journal of Korean medical science

Yoo J,Jung JH,Lee MA,Seo KJ,Shim BY,Kim SH,Cho DG,Ahn MI,Kim CH,Cho KD,Kang SJ,Kim HK

33-7000 was used in immunohistochemistry - paraffin section to study the immunohistochemistry of non-small cell lung cancer

Sun Apr 01 00:00:00 EDT 2007

Activation of p53-dependent growth suppression in human cells by mutations in PTEN or PIK3CA.

Molecular and cellular biology

Kim JS,Lee C,Bonifant CL,Ressom H,Waldman T

33-7000 was used in western blot to study the effect of PTEN and PIK3CA mutations on the activation of p53-dependent growth suppression in human cells

Mon Jan 01 00:00:00 EST 2007

Potent iron chelators increase the mRNA levels of the universal cyclin-dependent kinase inhibitor p21(CIP1/WAF1), but paradoxically inhibit its translation: a potential mechanism of cell cycle dysregulation.

Carcinogenesis

Le NT,Richardson DR

Sun Jun 01 00:00:00 EDT 2003

Genome-wide expression profiling of 8-chloroadenosine- and 8-chloro-cAMP-treated human neuroblastoma cells using radioactive human cDNA microarray.

Experimental & molecular medicine

Park GH,Choe J,Choo HJ,Park YG,Sohn J,Kim MK

33-7000 was used in western blot to analyze the cellular effects of 8-Cl-cAMP and 8-Cl-adenosine on human neuroblastoma cells using DNA arrays.

Wed Jul 31 00:00:00 EDT 2002

Bioinformatics

Protein Aliases: Activating Fragment 1; CDK-interacting protein 1; CDK-interaction protein 1; CDNK1A; Cip1); Cyclin-dependent kinase inhibitor 1; Cyclin-dependent kinase inhibitor 1A (p21; cyclin-dependent kinase inhibitor 1A (p21, Cip1); DNA synthesis inhibitor; MDA-6; melanoma differentiation associated protein 6; Melanoma differentiation-associated protein 6; OTTHUMP00000016298; p21; p21Cip1/Waf1; SLC12A9; Wild type p53 activated fragment 1 (WAF1); wild-type p53-activated fragment 1

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Gene Aliases: CAP20; CDKN1; CDKN1A; CIP1; MDA-6; MDA6; P21; p21CIP1; PIC1; SDI1; WAF1

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UniProt ID: (Human) P38936

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Entrez Gene ID: (Human) 1026

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Function(s)

cyclin-dependent protein serine/threonine kinase inhibitor activity protein binding cyclin-dependent protein kinase activating kinase activity cyclin binding ubiquitin protein ligase binding protein complex binding metal ion binding protein-binding activity modulator kinase inhibitor kinase modulator

Process(es)

regulation of cyclin-dependent protein serine/threonine kinase activity G1/S transition of mitotic cell cycle G2/M transition of mitotic cell cycle cellular response to DNA damage stimulus DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest cell cycle arrest Ras protein signal transduction negative regulation of cell proliferation response to toxic substance response to X-ray response to organonitrogen compound negative regulation of gene expression negative regulation of cell growth positive regulation of B cell proliferation organ regeneration cellular response to extracellular stimulus regulation of protein import into nucleus, translocation cellular response to amino acid starvation cellular response to heat negative regulation of phosphorylation response to drug intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator negative regulation of apoptotic process positive regulation of programmed cell death negative regulation of cyclin-dependent protein serine/threonine kinase activity response to arsenic-containing substance positive regulation of fibroblast proliferation protein stabilization response to corticosterone response to hyperoxia intestinal epithelial cell maturation cellular response to ionizing radiation cellular response to UV-B mitotic cell cycle arrest cellular senescence replicative senescence stress-induced premature senescence intrinsic apoptotic signaling pathway positive regulation of cyclin-dependent protein kinase activity negative regulation of G1/S transition of mitotic cell cycle regulation of DNA biosynthetic process positive regulation of reactive oxygen species metabolic process

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p21 Monoclonal Antibody (EA10)

p21 Antibody (33-7000) in ICC/IF

Product Details

33-7000

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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