Invitrogen

p21 Chimeric Recombinant Rabbit Monoclonal Antibody (Y13-238)

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-51677

Applications
Tested Dilution
Publications

Immunocytochemistry (ICC/IF)

10 µg/mL
-

Flow Cytometry (Flow)

1:100
-

Immunoprecipitation (IP)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG, lambda

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

Y13-238

Immunogen

Ha-MuSV transformed NRK cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

Product Specific Information

The antibody reacts with v-H-ras (within amino acids 120-138) and rodent c-H-ras p21's but not v-K-ras or rodent c-K-ras p21's. This antibody also recognizes human c-H-ras and c-K-ras p21's, but not human c-N-ras.

Preparation: Injection of rats subcutaneously with Ha-MuSV transformed NRK cells and screening of sera of individual animals to identify those with the strongest immune response against p21. Spleens were removed from the immunised animals and cell fusion of immune lymphocytes with rat myeloma cell line 210RCY3 Ag 1.2.3 (Y3) was carried out. Supernatants were harvested and assayed for antibodies to p21 by GDP binding assay or immunofluorescence on Ha-SV-transformed MDCK cells, followed by testing for the ability of the antibodies to immunoprecipitate p21.

Target Information

Protein p21 is a tumor suppressor protein which is an inhibitor of Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53. P21 is important in the response of cells to genotoxic stress and a major transcriptional target of p53 protein. The occurrence of p21 in the nucleus executes binding and inhibition activity of cyclin dependent kinases Cdk1 and Cdk2, and blocks the transition from G1 phase into S phase or from G2 phase into mitosis after DNA damage, which enables the repair of damaged DNA. In the cytoplasm, p21 protein has an anti-apoptotic effect. P21 is able to bind to and inhibit caspase 3, as well as the apoptotic kinases ASK1 and JNK. P21 exhibits a dual function in carcinogenesis, and acts as a tumor suppressor, prevents apoptosis, and acts as an oncogene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Activating Fragment 1; CDK-interacting protein 1; CDK-interaction protein 1; CDNK1A; Cip1); Cyclin-dependent kinase inhibitor 1; Cyclin-dependent kinase inhibitor 1A (p21; cyclin-dependent kinase inhibitor 1A (p21, Cip1); cyclin-dependent kinase inhibitor 1A variant 1; cyclin-dependent kinase inhibitor 1A variant 2; DNA synthesis inhibitor; MDA-6; melanoma differentiation associated protein 6; Melanoma differentiation-associated protein; Melanoma differentiation-associated protein 6; OTTHUMP00000016298; p21; p21; p21Cip1/Waf1; SLC12A9; Wild type p53 activated fragment 1 (WAF1); wild-type p53-activated fragment 1

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Gene Aliases: CAP20; CDKI; CDKN1; CDKN1A; CIP1; MDA-6; MDA6; P21; p21CIP1; p21WAF; PIC1; SDI1; WAF1

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UniProt ID: (Human) P38936, (Mouse) P39689

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Entrez Gene ID: (Human) 1026, (Mouse) 12575

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Function(s)
cyclin-dependent protein serine/threonine kinase inhibitor activity protein binding cyclin-dependent protein kinase activating kinase activity cyclin binding ubiquitin protein ligase binding protein complex binding metal ion binding protein kinase inhibitor activity protein-binding activity modulator kinase inhibitor kinase modulator
Process(es)
regulation of cyclin-dependent protein serine/threonine kinase activity G1/S transition of mitotic cell cycle G2/M transition of mitotic cell cycle cellular response to DNA damage stimulus DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest cell cycle arrest Ras protein signal transduction negative regulation of cell proliferation response to toxic substance response to X-ray response to organonitrogen compound negative regulation of gene expression negative regulation of cell growth positive regulation of B cell proliferation organ regeneration cellular response to extracellular stimulus regulation of protein import into nucleus, translocation cellular response to amino acid starvation cellular response to heat negative regulation of phosphorylation response to drug intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator negative regulation of apoptotic process positive regulation of programmed cell death negative regulation of cyclin-dependent protein serine/threonine kinase activity response to arsenic-containing substance positive regulation of fibroblast proliferation protein stabilization response to corticosterone response to hyperoxia intestinal epithelial cell maturation cellular response to ionizing radiation cellular response to UV-B mitotic cell cycle arrest cellular senescence replicative senescence stress-induced premature senescence intrinsic apoptotic signaling pathway positive regulation of cyclin-dependent protein kinase activity negative regulation of G1/S transition of mitotic cell cycle regulation of DNA biosynthetic process positive regulation of reactive oxygen species metabolic process protein phosphorylation cell cycle regulation of mitotic cell cycle response to UV positive regulation of protein kinase activity regulation of cell cycle signal transduction by p53 class mediator
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