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p27 Kip1 Antibody (MA5-37657) in IHC (P)
Immunohistochemistry (Paraffin) analysis of p27 Kip1 in paraffin-embedded human skeletal muscle sections using the p27 Kip1 Monoclonal Antibody (Product # MA5-37657) at a dilution of 1:25. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Product Details
MA5-37657
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
1373CT407.103.103
Immunogen
CDKN1B recombinant protein
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2897582
Target Information
p27 Kip1 functions as a negative regulator of G1 mouse expression and has been proposed to function as a possible mediator of TGFb induced G1 arrest. p27 Kip1 is an important regulator of cell cycle progression, a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. p27 Kip1 acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. p27 Kip1 has a molecular weight of approximately 27kDa and the degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDN1B; Cyclin-dependent kinase inhibitor 1B; cyclin-dependent kinase inhibitor 1B (p27, Kip1); Cyclin-dependent kinase inhibitor p27; Cyclin-dependent kinase inhibitor p27 Kip1; P27 CDKN1B; p27(KIP1); p27Kip1
Gene Aliases: CDKN1B; CDKN4; KIP1; MEN1B; MEN4; p27; P27KIP1
UniProt ID: (Human) P46527
Entrez Gene ID: (Human) 1027
cyclin-dependent protein serine/threonine kinase inhibitor activity
transforming growth factor beta receptor, cytoplasmic mediator activity
protein binding
cysteine-type endopeptidase activator activity involved in apoptotic process
protein phosphatase binding
Hsp70 protein binding
protein complex binding
chaperone binding
kinase inhibitor
regulation of cyclin-dependent protein serine/threonine kinase activity
G1/S transition of mitotic cell cycle
response to hypoxia
potassium ion transport
activation of cysteine-type endopeptidase activity involved in apoptotic process
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
cell cycle arrest
Notch signaling pathway
sensory perception of sound
positive regulation of cell proliferation
negative regulation of cell proliferation
response to glucose
positive regulation of cell death
negative regulation of cell growth
positive regulation of microtubule polymerization
response to estradiol
negative regulation of kinase activity
negative regulation of phosphorylation
response to drug
negative regulation of apoptotic process
response to amino acid
response to peptide hormone
positive regulation of protein catabolic process
negative regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of cell cycle
negative regulation of transcription, DNA-templated
negative regulation of mitotic cell cycle
response to cadmium ion
autophagic cell death
inner ear development
negative regulation of cellular component movement
negative regulation of epithelial cell proliferation involved in prostate gland development
cellular response to antibiotic
cellular response to lithium ion
cellular response to organic cyclic compound
mitotic cell cycle arrest
negative regulation of vascular smooth muscle cell proliferation
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