NeoBiotechnologies

p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Recombinant Rabbit Monoclonal Antibody (KIP1/1355R)

View all (84) p27Kip1 antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

1027-RBM5-P1

Applications
Tested Dilution
Publications

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

KIP1/1355R

Immunogen

Recombinant full-length human CDKN1B protein
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

Protein A/G

Storage buffer

PBS with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Antibody is stable for 24 months.

Positive Control: HeLa or MCF7 cells. Human tonsil, breast, cervical or colon carcinoma. Cellular Localization: Nuclear

Specificity Comments: This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.

Target Information

p27 Kip1 functions as a negative regulator of G1 mouse expression and has been proposed to function as a possible mediator of TGFb induced G1 arrest. p27 Kip1 is an important regulator of cell cycle progression, a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. p27 Kip1 acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. p27 Kip1 has a molecular weight of approximately 27kDa and the degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CDN1B; Cyclin-dependent kinase inhibitor 1B; cyclin-dependent kinase inhibitor 1B (p27, Kip1); Cyclin-dependent kinase inhibitor p27; Cyclin-dependent kinase inhibitor p27 Kip1; P27 CDKN1B; p27(KIP1); p27Kip1

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Gene Aliases: CDKN1B; CDKN4; KIP1; MEN1B; MEN4; p27; P27KIP1

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UniProt ID: (Human) P46527

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Entrez Gene ID: (Human) 1027

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Function(s)
cyclin-dependent protein serine/threonine kinase inhibitor activity transforming growth factor beta receptor, cytoplasmic mediator activity protein binding cysteine-type endopeptidase activator activity involved in apoptotic process protein phosphatase binding Hsp70 protein binding protein complex binding chaperone binding kinase inhibitor
Process(es)
regulation of cyclin-dependent protein serine/threonine kinase activity G1/S transition of mitotic cell cycle response to hypoxia potassium ion transport activation of cysteine-type endopeptidase activity involved in apoptotic process DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest cell cycle arrest Notch signaling pathway sensory perception of sound positive regulation of cell proliferation negative regulation of cell proliferation response to glucose positive regulation of cell death negative regulation of cell growth positive regulation of microtubule polymerization response to estradiol negative regulation of kinase activity negative regulation of phosphorylation response to drug negative regulation of apoptotic process response to amino acid response to peptide hormone positive regulation of protein catabolic process negative regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of cell cycle negative regulation of transcription, DNA-templated negative regulation of mitotic cell cycle response to cadmium ion autophagic cell death inner ear development negative regulation of cellular component movement negative regulation of epithelial cell proliferation involved in prostate gland development cellular response to antibiotic cellular response to lithium ion cellular response to organic cyclic compound mitotic cell cycle arrest negative regulation of vascular smooth muscle cell proliferation
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