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p38 MAPK alpha Antibody (MA5-45193) in IHC (PFA)
Immunohistochemistry analysis using colon carcinoma. Fixation involved formalin. Samples were incubated with p38 MAPK monoclonal antibody (Product # MA5-45193) at 1:10,000 for 12 hours at 4°C, followed by Biotin Goat Anti-Mouse at 1:2,000 for 1 hour at RT. Counterstain used was Mayer Hematoxylin (purple/blue) nuclear stain at 200 µL for 2 minutes at RT. Magnification: 40x.
Product Details
MA5-45193
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
9F12
Immunogen
Full length recombinant protein expressed in E.coli cells
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
9.1mM sodium bicarbonate/PBS, pH 7.4, with 581mM DMSO, 123mM ethanolamine
Contains
no preservative
Storage conditions
4° C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931647
Product Specific Information
Detects approximately 38kDa protein corresponding to p38 alpha MAPK when loaded with 6 ng of purified p38 alpha by chemiluminescent immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 38kDa.
Target Information
MAPK14 (p38 alpha) is a member of the stress-activated protein kinase class of MAPKs. The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CRK1; CSAID-binding protein; Csaids binding protein; cytokine suppressive anti-inflammatory drug binding protein; cytokine suppressive anti-inflammatory drug binding protein 1; Cytokine suppressive anti-inflammatory drug-binding protein; cytokine-supressive anti-inflammatory drug binding protein; EC 2.7.11.24; ERK1; MAP 14; MAP kinase 14; MAP kinase 2; MAP kinase MXI2; MAP kinase p38; MAP kinase p38 alpha; MAP kinase p38alpha; MAPK 14; MAPK1 antibody; MAX-interacting protein 2; mitogen activated protein kinase 14; Mitogen-activated protein kinase 14; mitogen-activated protein kinase 14A; Mitogen-activated protein kinase p38 alpha; MK14; OTTHUMP00000216563; OTTHUMP00000216566; p38 alpha; p38 MAP kinase; p38 MAP kinase alpha; p38 MAPK; p38 mitogen activated protein kinase; p38alpha Exip; reactive kinase; RP1-179N16.5; SAPK2a; Stress-activated protein kinase 2a; tRNA synthetase cofactor p38
Gene Aliases: CRK1; CSBP; CSBP1; CSBP2; CSPB1; EXIP; Hog; MAPK14; MXI2; p38; p38-alpha; p38a; p38ALPHA; p38Hog; p38MAPK; PRKM14; PRKM15; RK; SAPK2A
UniProt ID: (Human) Q16539, (Mouse) P47811
Entrez Gene ID: (Human) 1432, (Rat) 81649, (Mouse) 26416
protein serine/threonine kinase activity
MAP kinase activity
MAP kinase kinase activity
protein binding
ATP binding
protein C-terminus binding
enzyme binding
protein phosphatase binding
NFAT protein binding
protein kinase activity
kinase activity
nucleotide binding
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
DNA damage checkpoint
activation of MAPK activity
cell morphogenesis
cartilage condensation
angiogenesis
placenta development
chondrocyte differentiation
glucose metabolic process
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
apoptotic process
movement of cell or subcellular component
chemotaxis
signal transduction
cell surface receptor signaling pathway
transmembrane receptor protein serine/threonine kinase signaling pathway
Ras protein signal transduction
skeletal muscle tissue development
response to glucose
positive regulation of gene expression
positive regulation of myotube differentiation
myoblast differentiation involved in skeletal muscle regeneration
peptidyl-serine phosphorylation
fatty acid oxidation
osteoclast differentiation
positive regulation of cyclase activity
lipopolysaccharide-mediated signaling pathway
response to muramyl dipeptide
intracellular signal transduction
cellular response to vascular endothelial growth factor stimulus
response to muscle stretch
p38MAPK cascade
positive regulation of protein import into nucleus
signal transduction in response to DNA damage
positive regulation of blood vessel endothelial cell migration
positive regulation of erythrocyte differentiation
positive regulation of myoblast differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of glucose import
protein autophosphorylation
vascular endothelial growth factor receptor signaling pathway
regulation of sequence-specific DNA binding transcription factor activity
striated muscle cell differentiation
positive regulation of muscle cell differentiation
positive regulation of cardiac muscle cell proliferation
3'-UTR-mediated mRNA stabilization
cellular response to lipopolysaccharide
cellular response to ionizing radiation
negative regulation of canonical Wnt signaling pathway
positive regulation of brown fat cell differentiation
stress-induced premature senescence
cellular response to virus
regulation of cytokine production involved in inflammatory response
positive regulation of myoblast fusion
regulation of signal transduction by p53 class mediator
positive regulation of reactive oxygen species metabolic process
positive regulation of interleukin-12 secretion
MAPK cascade
regulation of transcription, DNA-templated
protein phosphorylation
cellular response to DNA damage stimulus
response to lipopolysaccharide
stress-activated MAPK cascade
phosphorylation
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