Invitrogen

p53 Monoclonal Antibody (DO-1)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

7 Published Figures

17 References

View all (369) p53 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

p53 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

p53 Antibody (AHO0152) in ICC/IF

Immunofluorescent analysis of p53 (green) in untreated and 5uM 20 hours Camptothecin treated HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes at -20c, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a p53 mouse monoclonal antibody (Product # AHO0152) at a concentration of 5 µg/mL in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor Plus 488 conjuga... View More

p53 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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p53 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein in p53 KO cell line compared to control cell line using Anti-p53 Monoclonal Antibody (DO-1) (Product # AHO0152). {KO}

Product Details

AHO0152

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

Assay-Dependent

View 4 publications 4 publications

Immunoprecipitation (IP)

Assay-Dependent

ChIP assay (ChIP)

1-3 µg x 10^6 cells

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Rhesus monkey

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

DO-1

Immunogen

Recombinant human wild type p53 protein expressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

-20°C

Shipping conditions

Dry ice

RRID

AB_2536306

Product Specific Information

This antibody reacts with mutant and wild form p53. It reacts with an epitope at aa 20-25.

Staining of formalin-fixed paraffin-embedded tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 20 min followed by cooling at room temperature for 20 min.

Recommended positive controls include SK-BR-3 cells, MDA-231 cells, and colon carcinoma.

Target Information

The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cell type-specific response of colon cancer tumor cell lines to oncolytic HSV-1 virotherapy in hypoxia.

Cancer cell international

Shayan S,Arashkia A,Bahramali G,Abdoli A,Nosrati MSS,Azadmanesh K

Published figure using p53 monoclonal antibody (Product # AHO0152) in Immunocytochemistry

Wed Apr 27 00:00:00 EDT 2022

ZFP14 Regulates Cancer Cell Growth and Migration by Modulating p53 Protein Stability as Part of the MDM2 E3 Ubiquitin Ligase Complex.

Cancers

Mohibi S,Chen X,Zhang J

AHO0152 was used in ChIP Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to suggest that the reciprocal regulation of p53 and ZFP14 represents a novel p53-ZFP14 regulatory loop involved in tumor suppression.

Tue Oct 25 00:00:00 EDT 2022

ZFP14 Regulates Cancer Cell Growth and Migration by Modulating p53 Protein Stability as Part of the MDM2 E3 Ubiquitin Ligase Complex.

Cancers

Mohibi S,Chen X,Zhang J

Tue Oct 25 00:00:00 EDT 2022

ZFP14 Regulates Cancer Cell Growth and Migration by Modulating p53 Protein Stability as Part of the MDM2 E3 Ubiquitin Ligase Complex.

Cancers

Mohibi S,Chen X,Zhang J

Tue Oct 25 00:00:00 EDT 2022

ZFP14 Regulates Cancer Cell Growth and Migration by Modulating p53 Protein Stability as Part of the MDM2 E3 Ubiquitin Ligase Complex.

Cancers

Mohibi S,Chen X,Zhang J

Tue Oct 25 00:00:00 EDT 2022

ZFP14 Regulates Cancer Cell Growth and Migration by Modulating p53 Protein Stability as Part of the MDM2 E3 Ubiquitin Ligase Complex.

Cancers

Mohibi S,Chen X,Zhang J

Tue Oct 25 00:00:00 EDT 2022

Phenotypic profiling with a living biobank of primary rhabdomyosarcoma unravels disease heterogeneity and AKT sensitivity.

Nature communications

Manzella G,Schreck LD,Breunis WB,Molenaar J,Merks H,Barr FG,Sun W,Römmele M,Zhang L,Tchinda J,Ngo QA,Bode P,Delattre O,Surdez D,Rekhi B,Niggli FK,Schäfer BW,Wachtel M

AHO0152 was used in Western Blotting to establish a biobank of PDX-derived primary rhabdomyosarcoma cultures.

Tue Sep 15 00:00:00 EDT 2020

Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies.

Cell reports

Mair B,Tomic J,Masud SN,Tonge P,Weiss A,Usaj M,Tong AHY,Kwan JJ,Brown KR,Titus E,Atkins M,Chan KSK,Munsie L,Habsid A,Han H,Kennedy M,Cohen B,Keller G,Moffat J

AHO0152 was used in Western Blotting to establish parameters for genome-wide screens in hPSCs to facilitate the characterization of unappreciated genetic regulators of hPSC biology.

Tue Apr 09 00:00:00 EDT 2019

High-Risk HPV, Biomarkers, and Outcome in Matched Cohorts of Head and Neck Cancer Patients Positive and Negative for HIV.

Molecular cancer research : MCR

Walline HM,Carey TE,Goudsmit CM,Bellile EL,D'Souza G,Peterson LA,McHugh JB,Pai SI,Lee JJ,Shin DM,Ferris RL

AHO0152 was used in immunohistochemistry - paraffin section to analyze high-risk HPV incidence, prognostic biomarkers, and outcome in HIV-positive and -negative patients with head and neck squamous cell cancer

Wed Feb 01 00:00:00 EST 2017

Alkaloids of fascaplysin are effective conventional chemotherapeutic drugs, inhibiting the proliferation of C6 glioma cells and causing their death in vitro.

Oncology letters

Bryukhovetskiy I,Lyakhova I,Mischenko P,Milkina E,Zaitsev S,Khotimchenko Y,Bryukhovetskiy A,Polevshchikov A,Kudryavtsev I,Khotimchenko M,Zhidkov M

AHO0152 was used in immunocytochemistry to compare the efficiency of fascaplysin with other drugs used to treat glial tumors

Wed Feb 01 00:00:00 EST 2017

Loss of ABHD5 promotes the aggressiveness of prostate cancer cells.

Scientific reports

Chen G,Zhou G,Aras S,He Z,Lucas S,Podgorski I,Skar W,Granneman JG,Wang J

AHO0152 was used in Western Blotting to provide evidence that ABHD5 acts as a metabolic tumor suppressor in PCa that prevents EMT and the Warburg effect.

Thu Oct 12 00:00:00 EDT 2017

Hematopoietic stem cells as a tool for the treatment of glioblastoma multiforme.

Molecular medicine reports

Bryukhovetskiy IS,Dyuizen IV,Shevchenko VE,Bryukhovetskiy AS,Mischenko PV,Milkina EV,Khotimchenko YS

AHO0152 was used in Immunocytochemistry-immunoflourescence to investigate the migration of human stem cells to cancer cells in vitro.

Tue Nov 01 00:00:00 EDT 2016

Cancer stem cells and microglia in the processes of glioblastoma multiforme invasive growth.

Oncology letters

Bryukhovetskiy I,Manzhulo I,Mischenko P,Milkina E,Dyuizen I,Bryukhovetskiy A,Khotimchenko Y

AHO0152 was used in immunocytochemistry clarify the interaction between microglial cells and cancer stem cells

Thu Sep 01 00:00:00 EDT 2016

Acquisition of Portal Venous Circulating Tumor Cells From Patients With Pancreaticobiliary Cancers by Endoscopic Ultrasound.

Gastroenterology

Catenacci DV,Chapman CG,Xu P,Koons A,Konda VJ,Siddiqui UD,Waxman I

AHO0152 was used in immunohistochemistry to assess a protocol for acquiring portal venous blood through endoscopic ultrasound

Tue Dec 01 00:00:00 EST 2015

MAP3K8/TPL-2/COT is a potential predictive marker for MEK inhibitor treatment in high-grade serous ovarian carcinomas.

Nature communications

Gruosso T,Garnier C,Abelanet S,Kieffer Y,Lemesre V,Bellanger D,Bieche I,Marangoni E,Sastre-Garau X,Mieulet V,Mechta-Grigoriou F

AHO0152 was used in western blot to identify and study roles for MAP3K8 in high-grade serous ovarian carcinomas

Mon Oct 12 00:00:00 EDT 2015

SIRT1 deacetylase is overexpressed in human melanoma and its small molecule inhibition imparts anti-proliferative response via p53 activation.

Archives of biochemistry and biophysics

Wilking MJ,Singh C,Nihal M,Zhong W,Ahmad N

Published figure using p53 monoclonal antibody (Product # AHO0152) in Western Blot

Mon Dec 01 00:00:00 EST 2014

Enhanced anticancer activity and circumvention of resistance mechanisms by novel polymeric/ phospholipidic nanocarriers of doxorubicin.

Journal of biomedical nanotechnology

Senkiv Y,Riabtseva A,Heffeter P,Boiko N,Kowol CR,Jungwith U,Shlyakhtina Y,Garasevych SG,Mitina N,Berger W,Zaichenko A,Stoika R

AHO0152 was used in western blot to describe a new polymeric-phospholipidic hybrid delivery system which enhanced the accumulation and activity of Dox in all tested cancer cell lines

Tue Jul 01 00:00:00 EDT 2014

Correlation of immunoglobulin G expression and histological subtype and stage in breast cancer.

PloS one

Yang B,Ma C,Chen Z,Yi W,McNutt MA,Wang Y,Korteweg C,Gu J

AHO0152 was used in immunohistochemistry - paraffin section to discuss the role of breast cancer-derived IgG in cancer development

Thu Oct 17 00:00:00 EDT 2013

p53 degradation activity, expression, and subcellular localization of E6 proteins from 29 human papillomavirus genotypes.

Journal of virology

Mesplède T,Gagnon D,Bergeron-Labrecque F,Azar I,Sénéchal H,Coutlée F,Archambault J

AHO0152 was used in western blot to develop a method to quantify p53 degradation activity of E6 in vivo and assess HPV variants.

Sun Jan 01 00:00:00 EST 2012

Mitochondrial p32 is a critical mediator of ARF-induced apoptosis.

Cancer cell

Itahana K,Zhang Y

AHO0152 was used in western blot to examine the role of mitochondrial p32 in ARF-induced apoptosis

Sun Jun 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: Antigen NY-CO-13; Cellular tumor antigen p53; Cys 51 Stop; FLJ92943; HGNC11998; mutant tumor protein 53; OTTMUSP00000006194; P53; p53 tumor suppressor; Phosphoprotein p53; transformation-related protein 53; tumor protein 53; Tumor suppressor p53; tumor supressor p53; Tumour Protein p53

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Gene Aliases: BCC7; EGK_08142; LFS1; P53; TP53; TRP53

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UniProt ID: (Human) P04637, (Rhesus monkey) P56424

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Entrez Gene ID: (Human) 7157, (Rhesus monkey) 716170

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Function(s)

RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter sequence-specific DNA binding RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding protease binding p53 binding DNA binding chromatin binding damaged DNA binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding copper ion binding protein binding ATP binding transcription factor binding zinc ion binding enzyme binding protein kinase binding protein phosphatase binding receptor tyrosine kinase binding ubiquitin protein ligase binding histone acetyltransferase binding identical protein binding sequence-specific DNA binding protein self-association transcription regulatory region DNA binding protein heterodimerization activity protein N-terminus binding chaperone binding protein phosphatase 2A binding P53-like transcription factor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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p53 Monoclonal Antibody (DO-1)

p53 Antibody (AHO0152) in ICC/IF

Product Details

AHO0152

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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