Invitrogen

p53 Monoclonal Antibody (PAb 1801)

Advanced Verification

IP-mass spectrometry

10 Published Figures

18 References

View all (369) p53 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

p53 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 13

p53 Antibody (13-4000) in IHC (P)

Immunohistochemistry analysis of p53 showing staining in the nucleus of paraffin-embedded human colon carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, wash... View More

p53 Antibody in Western Blot, ChIP Assay (WB, ChIP)

Product Image Gallery

p53 Antibody — IP-MS enrichment of TP53 (LFQ intensity): TP53 was enriched 3225-fold from BT549 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and TP53 antibody (Product # 13-4000). STRING database was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. {IP-MS}

Product Details

13-4000

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

ChIP assay (ChIP)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

PAb 1801

Immunogen

Recombinant p53 peptide.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533018

Target Information

The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CCNB1 Expedites the Progression of Cervical Squamous Cell Carcinoma via the Regulation by FOXM1.

OncoTargets and therapy

Li S,Liu N,Piao J,Meng F,Li Y

Published figure using p53 monoclonal antibody (Product # 13-4000) in Western Blot

Mon Oct 24 00:00:00 EDT 2022

CCNB1 Expedites the Progression of Cervical Squamous Cell Carcinoma via the Regulation by FOXM1.

OncoTargets and therapy

Li S,Liu N,Piao J,Meng F,Li Y

Published figure using p53 monoclonal antibody (Product # 13-4000) in Western Blot

Mon Oct 24 00:00:00 EDT 2022

Structure of the E6/E6AP/p53 complex required for HPV-mediated degradation of p53.

Nature

Martinez-Zapien D,Ruiz FX,Poirson J,Mitschler A,Ramirez J,Forster A,Cousido-Siah A,Masson M,Vande Pol S,Podjarny A,Travé G,Zanier K

13-4000 was used in western blot to report the crystal structure of the E6/E6AP/p53 complex

Thu Jan 28 00:00:00 EST 2016

Structure of the E6/E6AP/p53 complex required for HPV-mediated degradation of p53.

Nature

Martinez-Zapien D,Ruiz FX,Poirson J,Mitschler A,Ramirez J,Forster A,Cousido-Siah A,Masson M,Vande Pol S,Podjarny A,Travé G,Zanier K

13-4000 was used in western blot to report the crystal structure of the E6/E6AP/p53 complex

Thu Jan 28 00:00:00 EST 2016

Induction of LIFR confers a dormancy phenotype in breast cancer cells disseminated to the bone marrow.

Nature cell biology

Johnson RW,Finger EC,Olcina MM,Vilalta M,Aguilera T,Miao Y,Merkel AR,Johnson JR,Sterling JA,Wu JY,Giaccia AJ

134000 was used in western blot to propose that leukemia inhibitory factor signaling confers a dormancy phenotype in breast cancer cells that have disseminated to bone

Sat Oct 01 00:00:00 EDT 2016

Negative Regulation of p21Waf1/Cip1 by Human INO80 Chromatin Remodeling Complex Is Implicated in Cell Cycle Phase G2/M Arrest and Abnormal Chromosome Stability.

PloS one

Cao L,Ding J,Dong L,Zhao J,Su J,Wang L,Sui Y,Zhao T,Wang F,Jin J,Cai Y

13-4000 was used in western blot to show that the INO80 complex negatively regulates the p21Waf1/Cip1 expression in a p53-mediated mechanism.

Tue May 17 00:00:00 EDT 2016

Negative Regulation of p21Waf1/Cip1 by Human INO80 Chromatin Remodeling Complex Is Implicated in Cell Cycle Phase G2/M Arrest and Abnormal Chromosome Stability.

PloS one

Cao L,Ding J,Dong L,Zhao J,Su J,Wang L,Sui Y,Zhao T,Wang F,Jin J,Cai Y

13-4000 was used in western blot to show that the INO80 complex negatively regulates the p21Waf1/Cip1 expression in a p53-mediated mechanism.

Tue May 17 00:00:00 EDT 2016

Negative Regulation of p21Waf1/Cip1 by Human INO80 Chromatin Remodeling Complex Is Implicated in Cell Cycle Phase G2/M Arrest and Abnormal Chromosome Stability.

PloS one

Cao L,Ding J,Dong L,Zhao J,Su J,Wang L,Sui Y,Zhao T,Wang F,Jin J,Cai Y

13-4000 was used in western blot to show that the INO80 complex negatively regulates the p21Waf1/Cip1 expression in a p53-mediated mechanism.

Tue May 17 00:00:00 EDT 2016

Ceramide modulates pre-mRNA splicing to restore the expression of wild-type tumor suppressor p53 in deletion-mutant cancer cells.

Biochimica et biophysica acta

Patwardhan GA,Hosain SB,Liu DX,Khiste SK,Zhao Y,Bielawski J,Jazwinski SM,Liu YY

13-4000 was used in western blot to study the effect of ceramide treatment on p53

Sat Nov 01 00:00:00 EDT 2014

Chimeras of p14ARF and p16: functional hybrids with the ability to arrest growth.

PloS one

Williams RT,Barnhill LM,Kuo HH,Lin WD,Batova A,Yu AL,Diccianni MB

13-4000 was used in western blot to characterize a melanoma cell line with a novel deletion/substitution from CC to T in the shared exon 2 of p14ARF/p16

Mon Sep 29 00:00:00 EDT 2014

Resveratrol-mediated apoptosis of hodgkin lymphoma cells involves SIRT1 inhibition and FOXO3a hyperacetylation.

International journal of cancer

Frazzi R,Valli R,Tamagnini I,Casali B,Latruffe N,Merli F

13-4000 was used in western blot to study the cellular changes induced by resveratrol using Hodgkin lymphoma-derived L-428 cells.

Fri Mar 01 00:00:00 EST 2013

Increase in clusterin forms part of the stress response in Hodgkin's lymphoma.

International journal of oncology

Frazzi R,Casali B,Iori M,Nicoli D,Mammi C,Merli F

13-4000 was used in western blot to study clusterin in Hodgkin's lymphoma-derived cell lines.

Tue Mar 01 00:00:00 EST 2011

Cloning and characterization of the novel chimeric gene p53/FXR2 in the acute megakaryoblastic leukemia cell line CMK11-5.

The Tohoku journal of experimental medicine

Kanezaki R,Toki T,Xu G,Narayanan R,Ito E

13-4000 was used in western blot to identify and study a novel p53/an autosomal homolog of the fragile X mental retardation chimeric gene generated by an interstitial deletion

Sat Jul 01 00:00:00 EDT 2006

Heightened susceptibility to chronic gastritis, hyperplasia and metaplasia in Kcnq1 mutant mice.

Human molecular genetics

Elso CM,Lu X,Culiat CT,Rutledge JC,Cacheiro NL,Generoso WM,Stubbs LJ

13-4000 was used in immunohistochemistry - paraffin section to use Kcnq1 mutant mice to study factor that promote gastric cancer

Mon Nov 15 00:00:00 EST 2004

p73alpha regulation by Chk1 in response to DNA damage.

Molecular and cellular biology

Gonzalez S,Prives C,Cordon-Cardo C

13-4000 was used in Western blotting following immunoprecipitation to provide evidence that p73alpha is a target of checkpoint kinase 1.

Sat Nov 01 00:00:00 EST 2003

p73alpha regulation by Chk1 in response to DNA damage.

Molecular and cellular biology

Gonzalez S,Prives C,Cordon-Cardo C

13-4000 was used in Western blotting following immunoprecipitation to provide evidence that p73alpha is a target of checkpoint kinase 1.

Sat Nov 01 00:00:00 EST 2003

Expression of alternatively-spliced MDM2 transcripts in giant cell tumours of bone.

International journal of oncology

Evdokiou A,Atkins GJ,Bouralexis S,Hay S,Raggatt LJ,Cowled PA,Graves SE,Clayer M,Findlay DM

13-4000 was used in western blot to study the expression of MDM2 by the stromal and giant cells present in giant cell tumors of bone

Sat Sep 01 00:00:00 EDT 2001

Immunohistochemical analysis of candidate gene product expression in the duodenal epithelium of children with coeliac sprue.

Journal of clinical pathology

Barshack I,Goldberg I,Chowers Y,Weiss B,Horowitz A,Kopolovic J

13-4000 was used in immunohistochemistry (paraffin) to determine the immunoexpression of deleted in colon cancer, p53, E-cadherin, and beta-catenin in the duodenal mucosa of children with coeliac disease.

Sat Sep 01 00:00:00 EDT 2001

p53 represses androgen-induced transactivation of prostate-specific antigen by disrupting hAR amino- to carboxyl-terminal interaction.

The Journal of biological chemistry

Shenk JL,Fisher CJ,Chen SY,Zhou XF,Tillman K,Shemshedini L

13-4000 was used in western blot to investigate the relationship between androgen receptor and p53.

Fri Oct 19 00:00:00 EDT 2001

p53 represses androgen-induced transactivation of prostate-specific antigen by disrupting hAR amino- to carboxyl-terminal interaction.

The Journal of biological chemistry

Shenk JL,Fisher CJ,Chen SY,Zhou XF,Tillman K,Shemshedini L

13-4000 was used in western blot to investigate the relationship between androgen receptor and p53.

Fri Oct 19 00:00:00 EDT 2001

Bioinformatics

Protein Aliases: Antigen NY-CO-13; Cellular tumor antigen p53; Cys 51 Stop; FLJ92943; HGNC11998; mutant tumor protein 53; OTTMUSP00000006194; p53 tumor suppressor; Phosphoprotein p53; transformation-related protein 53; tumor protein 53; Tumor suppressor p53; tumor supressor p53; Tumour Protein p53

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Gene Aliases: BCC7; LFS1; P53; TP53; TRP53

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UniProt ID: (Human) P04637

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Entrez Gene ID: (Human) 7157

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Function(s)

RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter sequence-specific DNA binding RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding protease binding p53 binding DNA binding chromatin binding damaged DNA binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding copper ion binding protein binding ATP binding transcription factor binding zinc ion binding enzyme binding protein kinase binding protein phosphatase binding receptor tyrosine kinase binding ubiquitin protein ligase binding histone acetyltransferase binding identical protein binding sequence-specific DNA binding protein self-association transcription regulatory region DNA binding protein heterodimerization activity protein N-terminus binding chaperone binding protein phosphatase 2A binding P53-like transcription factor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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p53 Monoclonal Antibody (PAb 1801)

p53 Antibody (13-4000) in IHC (P)

Product Details

13-4000

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

ChIP assay (ChIP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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