Invitrogen

p53 Monoclonal Antibody (PAb 1801)

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IP-mass spectrometry

24 References

View all (369) p53 antibodies

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Datasheet

Protocols

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FIGURE: 1 / 7

p53 Antibody (MA5-11296) in WB

Western blot analysis was performed using nuclear enriched extracts (30 µg lysate) of MDA-MB-231 (Lane 1) and T-47D (Lane 2). The blots were probed with Anti-p53 Mouse Monoclonal Antibody (Product # MA5-11296, 0.5-2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A 53 kDa band corresponding to p53 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bi... View More

p53 Antibody in Immunoprecipitation (IP)

Product Image Gallery

p53 Antibody — IP-MS enrichment of TP53 (LFQ intensity): TP53 was enriched 2151-fold from BT549 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and TP53 antibody (Product # MA5-11296). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. {IP-MS}

Product Details

MA5-11296

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

2 µg/mL

ChIP assay (ChIP)

5 µg

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

PAb 1801

Immunogen

Recombinant Human p53 protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10981054

Product Specific Information

MA5-11296 targets p53 in FACS, IP, and WB applications and shows reactivity with Human samples.

The MA5-11296 immunogen is recombinant Human p53 protein.

Target Information

The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mitochondrial ferritin, a new target for inhibiting neuronal tumor cell proliferation.

Cellular and molecular life sciences : CMLS

Shi ZH,Shi FF,Wang YQ,Sheftel AD,Nie G,Zhao YS,You LH,Gou YJ,Duan XL,Zhao BL,Xu HM,Li CY,Chang YZ

MA5-11296 was used in western blot to characterize inhibition of neuronal tumor cell proliferation by mitochondrial ferritin

Sun Mar 01 00:00:00 EST 2015

Chimeras of p14ARF and p16: functional hybrids with the ability to arrest growth.

PloS one

Williams RT,Barnhill LM,Kuo HH,Lin WD,Batova A,Yu AL,Diccianni MB

MA5-11296 was used in western blot to characterize a melanoma cell line with a novel deletion/substitution from CC to T in the shared exon 2 of p14ARF/p16

Mon Sep 29 00:00:00 EDT 2014

Resveratrol-mediated apoptosis of hodgkin lymphoma cells involves SIRT1 inhibition and FOXO3a hyperacetylation.

International journal of cancer

Frazzi R,Valli R,Tamagnini I,Casali B,Latruffe N,Merli F

MA5-11296 was used in western blot to study the cellular changes induced by resveratrol using Hodgkin lymphoma-derived L-428 cells.

Fri Mar 01 00:00:00 EST 2013

Clinicopathological significance of cathepsin D expression in non-small cell lung cancer is conditional on apoptosis-associated protein phenotype: an immunohistochemistry study.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Fan C,Lin X,Wang E

MA5-11296 was used in immunohistochemistry to study the influence of the phenotype of apoptosis-associated proteins on the clinical significance of cathepsin D expression in non-small cell lung cancer

Wed Aug 01 00:00:00 EDT 2012

Increase in clusterin forms part of the stress response in Hodgkin's lymphoma.

International journal of oncology

Frazzi R,Casali B,Iori M,Nicoli D,Mammi C,Merli F

MA5-11296 was used in western blot to study clusterin in Hodgkin's lymphoma-derived cell lines.

Tue Mar 01 00:00:00 EST 2011

Prognostic significance of mucin and p53 expression in stage IB non-small cell lung cancer: a laboratory companion study to CALGB 9633.

Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer

Graziano SL,Gu L,Wang X,Tatum AH,Vollmer RT,Strauss GM,Kratzke R,Dudek AZ,Vokes EE,Green MR

MA5-11296 was used in immunohistochemistry to evaluate mucin and p53 expression as prognostic markers for stage IB non-small cell lung cancer

Tue Jun 01 00:00:00 EDT 2010

Diagnosing xeroderma pigmentosum group C by immunohistochemistry.

The American Journal of dermatopathology

de Feraudy S,Boubakour-Azzouz I,Fraitag S,Berneburg M,Chan L,Chew K,Clericuzio CL,Cunningham B,Tope WD,Cleaver JE

MA5-11296 was used in immunohistochemistry to develop an immunohistochemical method for the diagnosis of xeroderma pigmentosum group C

Thu Apr 01 00:00:00 EDT 2010

Functional four-base A/T gap core sequence CATTAG of P53 response elements specifically bound tetrameric P53 differently than two-base A/T gap core sequence CATG bound both dimeric and tetrameric P53.

Nucleic acids research

Cai BH,Chen JY,Lu MH,Chang LT,Lin HC,Chang YM,Chao CF

MA5-11296 was used in western blot to study the binding of tetrameric and dimeric p53 to two-base or four-base A/T gap core sequences

Wed Apr 01 00:00:00 EDT 2009

The ubiquitin-proteasome system regulates p53-mediated transcription at p21waf1 promoter.

Oncogene

Zhu Q,Wani G,Yao J,Patnaik S,Wang QE,El-Mahdy MA,Praetorius-Ibba M,Wani AA

MA5-11296 was used in ChIP assay, immunoprecipitation, and western blot to study the role of the ubiquitin-proteasome system in the transcriptional activation function of p53

Thu Jun 21 00:00:00 EDT 2007

H pylori status and angiogenesis factors in human gastric carcinoma.

World journal of gastroenterology

Mangia A,Chiriatti A,Ranieri G,Abbate I,Coviello M,Simone G,Zito FA,Montemurro S,Rucci A,Di Leo A,Tommasi S,Berloco P,Xu JM,Paradiso A

MA5-11296 was used in immunohistochemistry to study Helicobacter pylori antigen and angiogenesis factors in human gastric carcinoma

Thu Sep 14 00:00:00 EDT 2006

Altered gene expression induced by ionizing radiation and glycolytic inhibitor 2-deoxy-glucose in a human glioma cell line: implications for radio sensitization.

Cancer biology & therapy

Heminger K,Jain V,Kadakia M,Dwarakanath B,Berberich SJ

MA5-11296 was used in western blot to study the effects of ionizing radiation and 2-deoxyglucose on the transcriptome of a malignant glioma cell line and the significance for 2-deoxyglucose-induced radiosensitization

Sat Jul 01 00:00:00 EDT 2006

Cloning and characterization of the novel chimeric gene p53/FXR2 in the acute megakaryoblastic leukemia cell line CMK11-5.

The Tohoku journal of experimental medicine

Kanezaki R,Toki T,Xu G,Narayanan R,Ito E

MA5-11296 was used in western blot to identify and study a novel p53/an autosomal homolog of the fragile X mental retardation chimeric gene generated by an interstitial deletion

Sat Jul 01 00:00:00 EDT 2006

Conditionally replicative adenovirus expressing degradation-resistant p53 for enhanced oncolysis of human cancer cells overexpressing murine double minute 2.

Molecular cancer therapeutics

van Beusechem VW,van den Doel PB,Gerritsen WR

MA5-11296 was used in western blot to study the therapeutic potential of adenovirus encoding degradation-resistant p53 variants for enhanced oncolysis of cancer cells

Wed Jun 01 00:00:00 EDT 2005

MdmX represses E2F1 transactivation.

Cell cycle (Georgetown, Tex.)

Wunderlich M,Ghosh M,Weghorst K,Berberich SJ

MA5-11296 was used in western blot to study the p53- and Mdm2-independent inhibition of E2F1 transactivation by MdmX

Thu Apr 01 00:00:00 EST 2004

Heightened susceptibility to chronic gastritis, hyperplasia and metaplasia in Kcnq1 mutant mice.

Human molecular genetics

Elso CM,Lu X,Culiat CT,Rutledge JC,Cacheiro NL,Generoso WM,Stubbs LJ

MA5-11296 was used in immunohistochemistry - paraffin section to use Kcnq1 mutant mice to study factor that promote gastric cancer

Mon Nov 15 00:00:00 EST 2004

Involvement of reactive oxygen species in adaphostin-induced cytotoxicity in human leukemia cells.

Blood

Chandra J,Hackbarth J,Le S,Loegering D,Bone N,Bruzek LM,Narayanan VL,Adjei AA,Kay NE,Tefferi A,Karp JE,Sausville EA,Kaufmann SH

MA5-11296 was used in western blot to study the role of reactive oxygen species in adaphostin-induced cytotoxicity in human leukemia cells

Mon Dec 15 00:00:00 EST 2003

In vitro schedule-dependent interaction between docetaxel and gemcitabine in human gastric cancer cell lines.

Clinical cancer research : an official journal of the American Association for Cancer Research

Ricotti L,Tesei A,De Paola F,Ulivi P,Frassineti GL,Milandri C,Amadori D,Zoli W

MA5-11296 was used in immunocytochemistry to assess the activity of clinically used drugs and define the most effective treatment scheme in human gastric cancer cell lines

Sat Feb 01 00:00:00 EST 2003

G(1) and G(2) cell-cycle arrest following microtubule depolymerization in human breast cancer cells.

The Journal of clinical investigation

Blajeski AL,Phan VA,Kottke TJ,Kaufmann SH

MA5-11296 was used in western blot to study the effect of microtubule-destabilizing agents on cell-cycle arrest in human breast cancer cells

Mon Jul 01 00:00:00 EDT 2002

The predictive value of neuroendocrine markers and p53 for response to chemotherapy and survival in patients with advanced non-small cell lung cancer.

Lung cancer (Amsterdam, Netherlands)

Gajra A,Tatum AH,Newman N,Gamble GP,Lichtenstein S,Rooney MT,Graziano SL

MA5-11296 was used in immunohistochemistry to study the value of neuroendocrine markers and p53 for predicting chemotherapeutic response and survival in advanced non-small cell lung cancer

Wed May 01 00:00:00 EDT 2002

Expression of alternatively-spliced MDM2 transcripts in giant cell tumours of bone.

International journal of oncology

Evdokiou A,Atkins GJ,Bouralexis S,Hay S,Raggatt LJ,Cowled PA,Graves SE,Clayer M,Findlay DM

MA5-11296 was used in western blot to study the expression of MDM2 by the stromal and giant cells present in giant cell tumors of bone

Sat Sep 01 00:00:00 EDT 2001

Immunohistochemical analysis of candidate gene product expression in the duodenal epithelium of children with coeliac sprue.

Journal of clinical pathology

Barshack I,Goldberg I,Chowers Y,Weiss B,Horowitz A,Kopolovic J

MA5-11296 was used in immunohistochemistry (paraffin) to determine the immunoexpression of deleted in colon cancer, p53, E-cadherin, and beta-catenin in the duodenal mucosa of children with coeliac disease.

Sat Sep 01 00:00:00 EDT 2001

Derivation and initial characterization of a mouse mammary tumor cell line carrying the polyomavirus middle T antigen: utility in the development of novel cancer therapeutics.

Cancer research

Nielsen LL,Gurnani M,Shi B,Terracina G,Johnson RC,Carroll J,Mathis JM,Hajian G

MA5-11296 was used in western blot to generate and characterize a mouse mammary tumor cell line with polyomavirus middle T antigen

Fri Dec 15 00:00:00 EST 2000

Physical interaction and functional antagonism between the RNA polymerase II elongation factor ELL and p53.

The Journal of biological chemistry

Shinobu N,Maeda T,Aso T,Ito T,Kondo T,Koike K,Hatakeyama M

MA5-11296 was used in immunocytochemistry and immunoprecipitation to study the interaction and functional antagonism between the RNA polymerase II elongation factor ELL and p53

Fri Jun 11 00:00:00 EDT 1999

E7 protein of human papilloma virus-16 induces degradation of retinoblastoma protein through the ubiquitin-proteasome pathway.

Cancer research

Boyer SN,Wazer DE,Band V

MA5-11296 was used in western blot to study the role of the ubiquitin-proteasome pathway in the mechanism by which the HPV-16 E7 protein induces retinoblastoma protein degradation

Tue Oct 15 00:00:00 EDT 1996

Bioinformatics

Protein Aliases: Antigen NY-CO-13; Cellular tumor antigen p53; Cys 51 Stop; FLJ92943; HGNC11998; mutant tumor protein 53; OTTMUSP00000006194; p53 tumor suppressor; Phosphoprotein p53; transformation-related protein 53; tumor protein 53; Tumor suppressor p53; tumor supressor p53; Tumour Protein p53

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Gene Aliases: BCC7; LFS1; P53; TP53; TRP53

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UniProt ID: (Human) P04637

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Entrez Gene ID: (Human) 7157

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Function(s)

RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter sequence-specific DNA binding RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding protease binding p53 binding DNA binding chromatin binding damaged DNA binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding copper ion binding protein binding ATP binding transcription factor binding zinc ion binding enzyme binding protein kinase binding protein phosphatase binding receptor tyrosine kinase binding ubiquitin protein ligase binding histone acetyltransferase binding identical protein binding sequence-specific DNA binding protein self-association transcription regulatory region DNA binding protein heterodimerization activity protein N-terminus binding chaperone binding protein phosphatase 2A binding P53-like transcription factor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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p53 Monoclonal Antibody (PAb 1801)

p53 Antibody (MA5-11296) in WB

Product Details

MA5-11296

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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