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Invitrogen
pro-Caspase 3 Monoclonal Antibody (31A893)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 10
pro-Caspase 3 Antibody (MA1-41163) in ICC/IF
Immunofluorescence analysis of pro-Caspase 3 Monoclonal Antibody was performed using 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with pro-Caspase 3 (31A893) Mouse Monoclonal Antibody (Product # MA1-41163) at 2 µg/mL in 0.1... View More
Product Details
MA1-41163
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG
Class
Monoclonal
Type
Antibody
Clone
31A893
Immunogen
Recombinant full-length human Caspase-3.
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2070045
Product Specific Information
Suggested positive control: HL-60 or staurosporine treated apoptotic HeLa.
Target Information
Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase-3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein. Caspase-3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase-3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase-3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Apopain; CASP-3; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; Caspase-3; CPP-32; Cysteine protease CPP32; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; SREBP cleavage activity 1
Gene Aliases: CASP3; CPP32; CPP32B; SCA-1
UniProt ID: (Human) P42574
Entrez Gene ID: (Human) 836
protease binding
aspartic-type endopeptidase activity
cysteine-type endopeptidase activity
cyclin-dependent protein serine/threonine kinase inhibitor activity
death receptor binding
protein binding
peptidase activity
cysteine-type endopeptidase activator activity involved in apoptotic process
phospholipase A2 activator activity
protein complex binding
cysteine-type endopeptidase activity involved in apoptotic process
cysteine-type endopeptidase activity involved in execution phase of apoptosis
protease
response to hypoxia
B cell homeostasis
apoptotic DNA fragmentation
proteolysis
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
cellular component disassembly involved in execution phase of apoptosis
cellular response to DNA damage stimulus
heart development
sensory perception of sound
learning or memory
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
response to UV
response to glucose
response to X-ray
regulation of macroautophagy
protein processing
hippocampus development
neuron differentiation
keratinocyte differentiation
erythrocyte differentiation
platelet formation
negative regulation of B cell proliferation
response to cobalt ion
response to estradiol
response to lipopolysaccharide
glial cell apoptotic process
response to tumor necrosis factor
response to nicotine
hippo signaling
wound healing
response to drug
response to hydrogen peroxide
T cell homeostasis
negative regulation of apoptotic process
response to amino acid
positive regulation of neuron apoptotic process
cell fate commitment
negative regulation of cyclin-dependent protein serine/threonine kinase activity
negative regulation of activated T cell proliferation
response to antibiotic
neurotrophin TRK receptor signaling pathway
response to glucocorticoid
neuron apoptotic process
cellular response to organic cyclic compound
apoptotic signaling pathway
extrinsic apoptotic signaling pathway in absence of ligand
execution phase of apoptosis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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