Invitrogen

rRNA Monoclonal Antibody (Y10b)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3 References

View all (7) rRNA antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Advanced Verification (1)

rRNA Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 3

rRNA Antibody (MA1-13017) in ICC/IF

Immunofluorescence analysis of Ribosomal RNA was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Ribosomal RNA 5.8S (Y10B) Mouse Monoclonal Antibody (Product # MA1-13017) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conj... View More

Product Image Gallery

rRNA Antibody — Figure 2. Axonal Application of FGF22 Induces Synapse Formation and RibPs and rRNA 5.8S Decrease (A) Schematic representation of microfluidic chambers. (B) Representative image of spinal motor neurons (MNs) cultured in microfluidic chambers. At DIV 4, neurons were immunostained against MAP2 (somatodendritic marker; green) and Tau (axonal marker; red). (C and E) Presynaptic clusters increase upon FGF22 treatment. At DIV 3/4, MN axons were stimulated with either BSA (Ctr) or FGF22 (2 nM). At DIV 4, neurons were immunostained against synapsin (C) or SV2 (E) (green) and neurofilament (NF; red). Scale bar is 2.5 mum. (G, I, and K) Rib P and rRNA 5.8S decrease upon FGF22 treatment. At DIV 3/4, MN axons were stimulated with either BSA (Ctr) or FGF22 (2 nM). At DIV 4, neurons were immunostained against RibP P0 (G), RibP S6 (I), or rRNA 5.8S (K; green) and neurofilament (NF; red). Scale bar is 2.5 mum. (D, F, H, J, and L) Quantitative data of the number, area, and integrated density of synapsin (D) and SV2 (F) and number and area of RibP P0 (H) RibP S6 (J) and rRNA 5.8S (L) clusters per axonal length. Bars represent the mean +- SEM. Statistical significance by unpaired Student's t test. (M) Exogenous RibP EGFP-L10a decrease upon FGF22 treatment. MNs were transduced at DIV 1 with an EGFP-RibP L10a lentivirus and stimulated at DIV 3/4, with either BSA (Ctr) or FGF22 (2 nM). Neurons were immunostained against GFP (green) and neurofilament (NF; red). Scale bar is 2.5 mum. (N) Quantitative {TM}

Product Details

MA1-13017

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

Immunoprecipitation (IP)

Assay-dependent

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Many

Published species

Fruit fly, Mouse, Zebrafish

Host/Isotype

Mouse / IgG3, kappa

Class

Monoclonal

Type

Antibody

Clone

Y10b

Immunogen

Whole ribosomal rRNA 5.8S

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10979967

Product Specific Information

This clone recognizes both the large and the small subunit of rRNA from all eukaryotic cells.

MA1-13017 has been successfully used in Immunocytochemistry, immunoprecipitation, immunofluorescence applications.

The MA1-13017 immunogen is from whole ribosomal rRNA 52.8S

Target Information

rRNA (ribosomal RNA) forms a major structural component of ribosomes which provides a mechanism for decoding mRNA information into amino acids/proteins through the process of translation. rRNA plays a key role in accurate positioning of mRNA as well as peptidyl tRNA, and also plays a structural role in peptidyl transferase activity by generating scaffolds where ribosomal proteins are assembled. rRNA is generated inside nucleolus, which is localized in nucleus of the cells and contains genes essential for encoding rRNA. Defective rRNA may implicate in certain pathologies, for example: decreased rRNA expression has been observed in subjects with myelodysplastic syndromes, and abnormal rRNA expression has also been linked to Alzheimer's disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The transcription factor Xrp1 orchestrates both reduced translation and cell competition upon defective ribosome assembly or function.

eLife

Kiparaki M,Khan C,Folgado-Marco V,Chuen J,Moulos P,Baker NE

MA1-13017 was used in Immunohistochemistry (PFA fixed) to show that transcription factor Xrp1is the master regulator that triggers multiple consequences of ribosomal stresses and is the key instigator of cell competition.

Fri Feb 18 00:00:00 EST 2022

Role of ribosomal RNA released from red cells in blood coagulation in zebrafish and humans.

Blood advances

Alharbi A,Iyer N,Al Qaryoute A,Raman R,Burks DJ,Azad RK,Jagadeeswaran P

MA1-13017 was used in In vivo experiments to identify that a ribosomal RNA (rRNA) released in hemolysis activates clotting in human and zebrafish plasma and showed that fish hepatocyte growth factor activator (Hgfac) plays a role in rRNA-mediated activation of coagulation.

Tue Nov 23 00:00:00 EST 2021

Zonation of Ribosomal DNA Transcription Defines a Stem Cell Hierarchy in Colorectal Cancer.

Cell stem cell

Morral C,Stanisavljevic J,Hernando-Momblona X,Mereu E,Álvarez-Varela A,Cortina C,Stork D,Slebe F,Turon G,Whissell G,Sevillano M,Merlos-Suárez A,Casanova-Martí À,Moutinho C,Lowe SW,Dow LE,Villanueva A,Sancho E,Heyn H,Batlle E

MA1-13017 was used in Immunohistochemistry (Paraffin) to demonstrate the heterogenous biosynthetic functions of colorectal cancers.

Thu Jun 04 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: ribosomal RNA; rRNA

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rRNA Monoclonal Antibody (Y10b)

rRNA Antibody (MA1-13017) in ICC/IF

Product Details

MA1-13017

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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