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Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
The antiSerum does not cross-react with any other Human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
In immunoelectrophoresis in agarose-plates use 2 µL human saliva or equivalent against 120 µL antiserum. In double radial immunodiffusion use a rosette arrangement with 10 µL antiserum in 3 mm diameter center well and 2 µL plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4°C, not refrozen, and preferably used the same day.
Amylase is an enzyme that catalyses the breakdown of starch into sugars. Amylase is present in human saliva, where it begins the chemical process of digestion. By in situ hybridization combined with high resolution cytogenetics, the amylase gene is mapped to 1p21. Amylase enzymes find use in bread making and to break down complex sugars such as starch (found in flour) into simple sugars. Yeast then feeds on these simple sugars and converts it into the waste products of alcohol and CO2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 1,4-alpha-D-glucan glucanohydrolase 1; Alpha-amylase 1A; Alpha-amylase 1B; Alpha-amylase 1C; AMY2A; amylase, salivary, alpha-1A; glycogenase; Salivary alpha-amylase; salivary amylase; salivary amylase alpha 1A
Gene Aliases: AMY1; AMY1A; AMY1B; AMY1C
UniProt ID: (Human) P0DUB6
Entrez Gene ID: (Human) 276
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