FastDigest Enzyme Selection Tool | Thermo Fisher Scientific

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Catalog #	Product	Isoschizomers	Cut site and sequence	Overhang	Optimal reaction temperature	Thermal inactivation	Methylation Effects
FD0014	FastDigest AluI	AluBI	AG^CT TC^GA AGCT	blunt	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked if overlapped.
FD0024	FastDigest Alw21I	Bbv12I, BsiHKAI	GWGCW^C C^WCGWG GWGCWC	3’ protruding ends	37° C	80°C, 20 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD0034	FastDigest Alw26I	BsmAI, BstMAI	GTCTCN^NNNN CAGAGNNNNN^ GTCTCNNNNN	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect.
FD0044	FastDigest Alw44I	ApaLI, VneI	G^TGCAC CACGT^G GTGCAC	5’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect.
FD0054	FastDigest BamHI		G^GATCC CCTAG^G GGATCC	5’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD0055	FastDigest BamHI		G^GATCC CCTAG^G GGATCC	5’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD0064	FastDigest BcnI	AsuC2I, BpuMI, NciI	CC^SGG GGS^CC CCSGG	5’ protruding ends	37° C	80°C, 20 min	Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: no effect.
FD0074	FastDigest BglI		GCCNNNN^NGGC CGGN^NNNNCCG GCCNNNNNGGC	3’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect.
FD0083	FastDigest BglII		A^GATCT TCTAG^A AGATCT	5’ protruding ends	37° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: impaired if overlapped.
FD0084	FastDigest BglII		A^GATCT TCTAG^A AGATCT	5’ protruding ends	37° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: impaired if overlapped.
FD0094	FastDigest Bpu1102I	BlpI, Bsp1720I, CelII	GC^TNAGC CGANT^CG GCTNAGC	5’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD0124	FastDigest Bsp119I	AsuII, Bpu14I, BspT104I, BstBI, Csp45I, NspV, SfuI	TT^CGAA AAGC^TT TTCGAA	5’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoBI: no effect.
FD0134	FastDigest Bsp120I	PspOMI	G^GGCCC CCCGG^G GGGCCC	5’ protruding ends	37° C	80°C, 10 min	Dam: no effect. Dcm: blocked if overlapped. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect.
FD0143	FastDigest Bsu15I	BanIII, Bsa29I, BseCI, BshVI, BspDI, BspXI, BsuTUI, ClaI	AT^CGAT TAGC^TA ATCGAT	5’ protruding ends	37° C	65°C, 5 min	Dam: blocked if overlapped. Dcm: no effect. CpG: blocked. EcoKI: no effect.
FD0144	FastDigest Bsu15I	BanIII, Bsa29I, BseCI, BshVI, BspDI, BspXI, BsuTUI, ClaI	AT^CGAT TAGC^TA ATCGAT	5’ protruding ends	37° C	65°C, 5 min	Dam: blocked if overlapped. Dcm: no effect. CpG: blocked. EcoKI: no effect.
FD0154	FastDigest BsuRI	BshFI, BsnI, BspANI, HaeIII, PhoI	GG^CC CC^GG GGCC	blunt	37° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD0184	FastDigest Cfr10I	Bse118I, BsrFI, BssAI	R^CCGGY YGGCC^R RCCGGY	5’ protruding ends	37° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD0194	FastDigest Cfr13I	AspS9I, BmgT120I, PspPI, Sau96I	G^GNCC CCNG^G GGNCC	5’ protruding ends	37° C	No (column purification)	Dam: no effect. Dcm: blocked if overlapped. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect.
FD0214	FastDigest Csp6I	AfaI, CviQI, RsaNI	G^TAC CAT^G GTAC	5’ protruding ends	37° C	80°C, 10 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD0224	FastDigest DraI		TTT^AAA AAA^TTT TTTAAA	blunt	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: blocked if overlapped. EcoBI: no effect.
FD0234	FastDigest Eam1104I	Bst6I, EarI	CTCTTCN^NNN GAGAAGNNNN^ CTCTTCNNNN	5’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD0244	FastDigest Eam1105I	AhdI, AspEI, BmeRI, DriI, EclHKI	GACNNN^NNGTC CTGNN^NNNCAG GACNNNNNGTC	3’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD0254	FastDigest Ecl136II	Eco53kI, EcoICRI, Psp124BI, SstI	GAG^CTC CTC^GAG GAGCTC	blunt	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect.
FD0264	FastDigest EcoO109I	DraII	RG^GNCCY YCCNG^GR RGGNCCY	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: blocked if overlapped. CpG: no effect. EcoKI: no effect.
FD0274	FastDigest EcoRI		G^AATTC CTTAA^G GAATTC	5’ protruding ends	37° C	80°C, 5min	Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect.
FD0275	FastDigest EcoRI		G^AATTC CTTAA^G GAATTC	5’ protruding ends	37° C	80°C, 5min	Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect.
FD0293	FastDigest Eco31I	BsaI, Bso31I, BspTNI	GGTCTCN^NNNN CCAGAGNNNNN^ GGTCTCNNNNN	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: impaired if overlapped. CpG: impaired if overlapped. EcoKI: no effect.
FD0294	FastDigest Eco31I	BsaI, Bso31I, BspTNI	GGTCTCN^NNNN CCAGAGNNNNN^ GGTCTCNNNNN	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: impaired if overlapped. CpG: impaired if overlapped. EcoKI: no effect.
FD0303	FastDigest Eco32I	EcoRV	GAT^ATC CTA^TAG GATATC	blunt	37° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD0304	FastDigest Eco32I	EcoRV	GAT^ATC CTA^TAG GATATC	blunt	37° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD0314	FastDigest Eco47I	AvaII, Bme18I, SinI, VpaK11BI	G^GWCC CCWG^G GGWCC	5’ protruding ends	37° C	80°C, 20 min	Dam: no effect. Dcm: blocked if overlapped. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect.
FD0324	FastDigest Eco47III	AfeI, Aor51HI	AGC^GCT TCG^CGA AGCGCT	blunt	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect.
FD0334	FastDigest Eco52I	BseX3I, BstZI, EagI, EclXI	C^GGCCG GCCGG^C CGGCCG	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD0344	FastDigest Eco57I	AcuI, Bsp6II, BspD6II, BspKT5I, Eco112I, Eco125I, FsfI, SstE37III	CTGAAGNNNNNNNNNNNNNNNN^ GACTTCNNNNNNNNNNNNNN^NN CTGAAGNNNNNNNNNNNNNNNN	3’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD0364	FastDigest Eco72I	AcvI, BbrPI, PmaCI, PmlI, PspCI	CAC^GTG GTG^CAC CACGTG	blunt	37° C	80°C, 10 min	Dam: no effect. Dcm: no effect. CpG: blocked.
FD0374	FastDigest Eco81I	AxyI, Bse21I, Bsu36I	CC^TNAGG GGANT^CC CCTNAGG	5’ protruding ends	37° C	80°C, 10 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD0384	FastDigest Eco88I	Ama87I, AvaI, BmeT110I, BsiHKCI, BsoBIm	C^YCGRG GRGCY^C CYCGRG	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: no effect.
FD0394	FastDigest Eco91I	BstEII, BstPI, EcoO65I, PspEI	G^GTNACC CCANTG^G GGTNACC	5’ protruding ends	37° C	65°C, 10 min	Dam: no effect. Dcm: no effect. CpG: no effect.
FD0404	FastDigest Eco105I	BstSNI, SnaBI	TAC^GTA ATG^CAT TACGTA	blunt	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD0414	FastDigest Eco130I	BssT1I, EcoT14I, ErhI, StyI	C^CWWGG GGWWC^C CCWWGG	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD0424	FastDigest Eco147I	AatI, PceI, SseBI, StuI	AGG^CCT TCC^GGA AGGCCT	blunt	37° C	80°C, 10 min	Dam: no effect. Dcm: blocked if overlapped. CpG: no effect. EcoKI: no effect.
FD0434	FastDigest ScaI	AssI, BmcAI, ZrmI	AGT^ACT TCA^TGA AGTACT	blunt	37° C	65°C, 10 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked if overlapped.
FD0443	FastDigest EheI	BbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI	GGC^GCC CCG^CGG GGCGCC	blunt	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD0444	FastDigest EheI	BbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI	GGC^GCC CCG^CGG GGCGCC	blunt	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD0454	FastDigest Esp3I	BsmBI, BstGZ53I, Esp16I, Esp23I	CGTCTCN^NNNN GCAGAGNNNNN^ CGTCTCNNNNN	5’ protruding ends	37° C	65°C, 10 min	Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. EcoKI: no effect.
FD0464	FastDigest GsuI	Bco35I, BpmI, Bsp22I, Bsp28I, BspJ74I, Bth1795I, Uba1437I, Uba1444I	CTGGAGNNNNNNNNNNNNNNNN^ GACCTCNNNNNNNNNNNNNN^NN CTGGAGNNNNNNNNNNNNNNNN	3’ protruding ends	30° C	65°C, 5 min	Dam: no effect. Dcm: blocked if overlapped. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD0474	FastDigest Hin1I	AcyI, BsaHI, BssNI, BstACI, Hsp92I	GR^CGYC CYGC^RG GRCGYC	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: impaired if overlapped. CpG: blocked. EcoKI: no effect.
FD0484	FastDigest Hin6I	AspLEI, BstHHI, CfoI, HinP1I, HspAI	G^CGC CGC^G GCGC	5’ protruding ends	37° C	80°C, 10 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD0494	FastDigest HincII	HindII	GTY^RAC CAR^YTG GTYRAC	blunt	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: blocked if overlapped. EcoBI: blocked if overlapped.
FD0504	FastDigest HindIII		A^AGCTT TTCGA^A AAGCTT	5’ protruding ends	37° C	80°C, 10 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: impaired if overlapped.
FD0505	FastDigest HindIII		A^AGCTT TTCGA^A AAGCTT	5’ protruding ends	37° C	80°C, 10 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD0514	FastDigest HpaII	BsiSI, HapII	C^CGG GGC^C CCGG	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD0524	FastDigest KpnI	Asp718I	GGTAC^C C^CATGG GGTACC	3’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD0534	FastDigest Kpn2I	AccIII, Aor13HI, BseAI, Bsp13I, BspEI, MroI	T^CCGGA AGGCC^T TCCGGA	5’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD0544	FastDigest MspI	BsiSI, HapII	C^CGG GGC^C CCGG	5’ protruding ends	37° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD0554	FastDigest MvaI	AjnI, BseBI, Bst2UI, BstNI, BstOI, Psp6I, PspGI	CC^WGG GGW^CC CCWGG	5’ protruding ends	37° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD0564	FastDigest MluI		A^CGCGT TGCGC^A ACGCGT	5’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD0573	FastDigest NcoI	Bsp19I	C^CATGG GGTAC^C CCATGG	5’ protruding ends	37° C	65°C, 15 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD0574	FastDigest NcoI	Bsp19I	C^CATGG GGTAC^C CCATGG	5’ protruding ends	37° C	65°C, 15 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD0575	FastDigest NcoI	Bsp19I	C^CATGG GGTAC^C CCATGG	5’ protruding ends	37° C	65°C, 15 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD0583	FastDigest NdeI	FauNDI	CA^TATG GTAT^AC CATATG	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD0584	FastDigest NdeI	FauNDI	CA^TATG GTAT^AC CATATG	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD0585	FastDigest NdeI	FauNDI	CA^TATG GTAT^AC CATATG	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD0593	FastDigest NotI	CciNI	GC^GGCCGC CGCCGG^CG GCGGCCGC	5’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD0594	FastDigest NotI	CciNI	GC^GGCCGC CGCCGG^CG GCGGCCGC	5’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD0595	FastDigest NotI	CciNI	GC^GGCCGC CGCCGG^CG GCGGCCGC	5’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD0596	FastDigest NotI	CciNI	GC^GGCCGC CGCCGG^CG GCGGCCGC	5’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD0604	FastDigest PaeI	BbuI, SphI	GCATG^C C^GTACG GCATGC	3’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked if overlapped.
FD0614	FastDigest PstI	BspMAI	CTGCA^G G^ACGTC CTGCAG	3’ protruding ends	37° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD0615	FastDigest PstI	BspMAI	CTGCA^G G^ACGTC CTGCAG	3’ protruding ends	37° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD0624	FastDigest PvuI	BpvUI, MvrI, Ple19I	CGAT^CG GC^TAGC CGATCG	3’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD0634	FastDigest PvuII		CAG^CTG GTC^GAC CAGCTG	blunt	37° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD0644	FastDigest SalI		G^TCGAC CAGCT^G GTCGAC	5’ protruding ends	37° C	65°C, 10 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD0654	FastDigest SduI	Bsp1286I, MhlI	GDGCH^C C^HCGDG GDGCHC	3’ protruding ends	37° C	80°C, 15 min	Dam: no effect. Dcm: no effect. CpG: no effect.
FD0663	FastDigest SmaI	TspMI, XmaCI, XmaI	CCC^GGG GGG^CCC CCCGGG	blunt	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD0664	FastDigest SmaI	TspMI, XmaCI, XmaI	CCC^GGG GGG^CCC CCCGGG	blunt	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD0674	FastDigest TaqI		T^CGA AGC^T TCGA	5’ protruding ends	65° C	No (column purification)	Dam: blocked if overlapped. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD0684	FastDigest XbaI		T^CTAGA AGATC^T TCTAGA	5’ protruding ends	37° C	65°C, 20 min	Dam: blocked if overlapped. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD0685	FastDigest XbaI		T^CTAGA AGATC^T TCTAGA	5’ protruding ends	37° C	65°C, 20 min	Dam: blocked if overlapped. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD0694	FastDigest XhoI	PaeR7I, Sfr274I, SlaI, StrI, TliI	C^TCGAG GAGCT^C CTCGAG	5’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: no effect.
FD0695	FastDigest XhoI	PaeR7I, Sfr274I, SlaI, StrI, TliI	C^TCGAG GAGCT^C CTCGAG	5’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: no effect.
FD0704	FastDigest Bst1107I	BssNAI, BstZ17I	GTA^TAC CAT^ATG GTATAC	blunt	37° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect.
FD0714	FastDigest Van91I	AccB7I, BasI, PflMI	CCANNNN^NTGG GGTN^NNNNACC CCANNNNNTGG	3’ protruding ends	37° C	65°C, 10 min	Dam: no effect. Dcm: blocked if overlapped. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD0724	FastDigest BclI	FbaI, Ksp22I	T^GATCA ACTAG^T TGATCA	5’ protruding ends	37° C	No (column purification)	Dam: blocked. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked if overlapped.
FD0734	FastDigest Mph1103I	EcoT22I, NsiI, Zsp2I	ATGCA^T T^ACGTA ATGCAT	3’ protruding ends	37° C	65°C, 15 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD0744	FastDigest CpoI	CspI, Rsr2I, RsrII	CG^GWCCG GCCWG^GC CGGWCCG	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD0753	FastDigest MunI	MfeI	C^AATTG GTTAA^C CAATTG	5’ protruding ends	37° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD0754	FastDigest MunI	MfeI	C^AATTG GTTAA^C CAATTG	5’ protruding ends	37° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD0764	FastDigest Psp5II	PpuMI, PspPPI	RG^GWCCY YCCWG^GR RGGWCCY	5’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: blocked if overlapped. CpG: no effect. EcoKI: no effect.
FD0774	FastDigest SspI		AAT^ATT TTA^TAA AATATT	blunt	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD0784	FastDigest Bsp143I	BfuCI, BssMI, BstKTI, BstMBI, DpnII, Kzo9I, NdeII, Sau3AIm	^GATC CTAG^ GATC	5’ protruding ends	37° C	65°C, 20 min	Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect.
FD0804	FastDigest HinfI		G^ANTC CTNA^G GANTC	5’ protruding ends	37° C	65°C, 20 min	Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: blocked if overlapped.
FD0814	FastDigest MboI	BfuCI, BssMI, BstKTI, BstMBI, DpnII, Kzo9I, NdeII, Sau3AIm	^GATC CTAG^ GATC	5’ protruding ends	37° C	65°C, 15 min	Dam: blocked. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked if overlapped.
FD0824	FastDigest MboII		GAAGANNNNNNNN^ CTTCTNNNNNNN^N GAAGANNNNNNNN	3’ protruding ends	37° C	65°C, 5 min	Dam: blocked if overlapped. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD0834	FastDigest BspTI	AflII, BfrI, Bst98I, BstAFI, MspCI, Vha464I	C^TTAAG GAATT^C CTTAAG	5’ protruding ends	37° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD0854	FastDigest Pfl23II	BsiWI, PspLI	C^GTACG GCATG^C CGTACG	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD0874	FastDigest BseGI	BstF5I, BtsCI, FokI	GGATGNN^ CCTAC^NN GGATGNN	3’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD0884	FastDigest BseNI	Bse1I, BsrI, BsrSI	ACTGGN^ TGAC^CN ACTGGN	3’ protruding ends	65° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect.
FD0894	FastDigest Bsh1285I	BsiEI, BstMCI	CGRY^CG GC^YRGC CGRYCG	3’ protruding ends	37° C	80°C, 15 min	Dam: impaired if overlapped. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD0904	FastDigest Acc65I	Asp718I	G^GTACC CCATG^G GGTACC	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: impaired if overlapped. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect.
FD0914	FastDigest VspI	AseI, PshBI	AT^TAAT TAAT^TA ATTAAT	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD0924	FastDigest Bsh1236I	AccII, BspFNI, BstFNI, BstUI, MvnI	CG^CG GC^GC CGCG	blunt	37° C	80°C, 10 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD0933	FastDigest Bsp1407I	BsrGI, BstAUI	T^GTACA ACATG^T TGTACA	5’ protruding ends	37° C	80°C, 10 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD0934	FastDigest Bsp1407I	BsrGI, BstAUI	T^GTACA ACATG^T TGTACA	5’ protruding ends	37° C	80°C, 10 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD0944	FastDigest Psp1406I	AclI	AA^CGTT TTGC^AA AACGTT	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked.
FD0964	FastDigest Mva1269I	BsaMI, BsmI, PctI	GAATGCN^ CTTAC^GN GAATGCN	3’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD0973	FastDigest NheI	AsuNHI, BmtI	G^CTAGC CGATC^G GCTAGC	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect.
FD0974	FastDigest NheI	AsuNHI, BmtI	G^CTAGC CGATC^G GCTAGC	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect.
FD0984	FastDigest Tru1I	MseI, Tru9I	T^TAA AAT^T TTAA	5’ protruding ends	65° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: blocked if overlapped. EcoBI: no effect.
FD0994	FastDigest AatII	ZraI	GACGT^C C^TGCAG GACGTC	3’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD1004	FastDigest BshNI	AccB1I, BanI, BspT107I	G^GYRCC CCRYG^G GGYRCC	5’ protruding ends	37° C	65°C, 10 min	Dam: no effect. Dcm: impaired if overlapped. CpG: impaired if overlapped. EcoBI: no effect.
FD1014	FastDigest BpiI	BbsI, BpuAI, BstV2I	GAAGACNN^NNNN CTTCTGNNNNNN^ GAAGACNNNNNN	5’ protruding ends	37° C	65°C, 10 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD1024	FastDigest BstXI		CCANNNNN^NTGG GGTN^NNNNNACC CCANNNNNNTGG	3’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: impaired if overlapped. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD1034	FastDigest KspAI	HpaI	GTT^AAC CAA^TTG GTTAAC	blunt	37° C	65°C, 20 min	Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: blocked if overlapped. EcoBI: no effect.
FD1074	FastDigest MnlI		CCTCNNNNNNN^ GGAGNNNNNN^N CCTCNNNNNNN	3’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked if overlapped.
FD1084	FastDigest BseDI	BsaJI, BssECI	C^CNNGG GGNNC^C CCNNGG	5’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD1124	FastDigest RsaI	AfaI, CviQI, RsaNI	GT^AC CA^TG GTAC	blunt	37° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect.
FD1133	FastDigest SacI	Eco53kI, EcoICRI, Psp124BI, SstI	GAGCT^C C^TCGAG GAGCTC	3’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD1134	FastDigest SacI	Eco53kI, EcoICRI, Psp124BI, SstI	GAGCT^C C^TCGAG GAGCTC	3’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD1144	FastDigest TaiI	HpyCH4IV, MaeII	ACGT^ ^TGCA ACGT	3’ protruding ends	65° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: blocked.
FD1154	FastDigest BspLI	BmiI, NlaIV, PspN4I	GGN^NCC CCN^NGG GGNNCC	blunt	37° C	65°C, 20 min	Dam: no effect. Dcm: impaired if overlapped. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect.
FD1164	FastDigest BfmI	BstSFI, SfcI	C^TRYAG GAYRT^C CTRYAG	5’ protruding ends	37° C	65°C, 20 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD1184	FastDigest Bpu10I		CC^TNAGC GGANT^CG CCTNAGC	5’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD1194	FastDigest SdaI	SbfI, Sse8387I	CCTGCA^GG GG^ACGTCC CCTGCAGG	3’ protruding ends	37° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD1204	FastDigest BseLI	AfiI, Bsc4I, BslI	CCNNNNN^NNGG GGNN^NNNNNCC CCNNNNNNNGG	3’ protruding ends	37° C	No (column purification)	Dam: no effect. Dcm: impaired if overlapped. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect.
FD1214	FastDigest MlsI	BalI, MluNI, MscI, Msp20I	TGG^CCA ACC^GGT TGGCCA	blunt	37° C	80°C, 20 min	Dam: no effect. Dcm: blocked if overlapped. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD1224	FastDigest NsbI	Acc16I, AviII, FspI	TGC^GCA ACG^CGT TGCGCA	blunt	37° C	65°C, 15 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD1234	FastDigest AdeI	DraIII	CACNNN^GTG GTG^NNNCAC CACNNNGTG	3’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: impaired if overlapped.
FD1244	FastDigest SmiI	SwaI	ATTT^AAAT TAAA^TTTA ATTTAAAT	blunt	37° C	65°C, 15 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD1253	FastDigest BcuI	AhlI, SpeI	A^CTAGT TGATC^A ACTAGT	5’ protruding ends	37° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: no effect.
FD1254	FastDigest BcuI	AhlI, SpeI	A^CTAGT TGATC^A ACTAGT	5’ protruding ends	37° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: no effect.
FD1264	FastDigest BseMI	Bse3DI, BsrDI	GCAATGNN^ CGTTAC^NN GCAATGNN	3’ protruding ends	55° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD1274	FastDigest MbiI	AccBSI, BsrBI	CCG^CTC GGC^GAG CCGCTC	blunt	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect.
FD1284	FastDigest PagI	CciI, BspHI, RcaI	T^CATGA AGTAC^T TCATGA	5’ protruding ends	37° C	80°C, 5 min	Dam: impaired if overlapped. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: impaired if overlapped.
FD1294	FastDigest TatI		W^GTACW WCATG^W WGTACW	5’ protruding ends	65° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD1304	FastDigest XagI	BstENI, EcoNI	CCTNN^NNNAGG GGANNN^NNTCC CCTNNNNNAGG	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD1314	FastDigest BplI		GAAGACNN^NNNN CTTCTGNNNNNN^ GAAGACNNNNNN	3’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD1334	FastDigest PsyI	AspI, PflFI, Tth111I	GACN^NNGTC CTGNN^NCAG GACNNNGTC	5’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD1344	FastDigest MssI	PmeI	GTTT^AAAC CAAA^TTTG GTTT^AAAC	blunt	37° C	65°C, 10 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: blocked if overlapped. EcoBI: no effect.
FD1354	FastDigest TasI	Sse9I, Tsp509I, TspEI	^AATT TTAA^> AATT	5’ protruding ends	65° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked if overlapped.
FD1364	FastDigest TaaI	Bst4CI, HpyCH4III	ACN^GT TG^NCA ACNGT	3’ protruding ends	65° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: impaired if overlapped.
FD1374	FastDigest SchI	MlyI, PleI, PpsI, SchI	GAGTCNNNNN^ CTCAGNNNNN GAGTCNNNNN	blunt	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD1383	FastDigest XapI	AcsI, ApoI	R^AATTY YTTAA^R RAATTY	5’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD1384	FastDigest XapI	AcsI, ApoI	R^AATTY YTTAA^R RAATTY	5’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD1394	FastDigest CaiI	AlwNI	CAGNNN^CTG GTC^NNNGAC CAGNNNCTG	3’ protruding ends	37° C	65°C, 10 min	Dam: no effect. Dcm: blocked if overlapped. CpG: no effect. EcoKI: no effect.
FD1404	FastDigest BseMII	BspCNI	GCAATGNN^ CGTTAC^NN GCAATGNN	3’ protruding ends	55° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD1414	FastDigest ApaI	PspOMI	GGGCC^C C^CCGGG GGGCCC	3’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: impaired if overlapped. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect.
FD1424	FastDigest Bme1390I	BmrFI, BssKI, BstSCI, MspR9I, ScrFI, StyD4I	CC^NGG GGN^CC CCNGG	5’ protruding ends	37° C	No (column purification)	Dam: no effect. Dcm: blocked if overlapped. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect.
FD1434	FastDigest BoxI	BstPAI, PshAI	GACNN^NNGTC CTGNN^NNCAG GACNNNNGTC	blunt	37° C	80°C, 20 min	Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect.
FD1444	FastDigest BseSI	BaeGI, BstSLI	GKGCM^C C^MCGKG GKGCMC	3’ protruding ends	37° C	80°C, 15 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: impaired if overlapped. EcoBI: no effect.
FD1454	FastDigest BseXI	BbvI, BstV1I	GCAGCNNNNNNNN^NNNN CGTCGNNNNNNNNNNNN^ GCAGCNNNNNNNNNNNN	5’ protruding ends	65° C	80°C, 15 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD1464	FastDigest BshTI	AgeI, AsiGI, CspAI, PinAI	A^CCGGT TGGCC^A ACCGGT	5’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked.
FD1474	FastDigest XceI	BstNSI, NspI	RCATG^Y Y^GTACR RCATGY	3’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD1484	FastDigest XmiI	AccI, FblI	GT^MKAC CAKM^TG GTMKAC	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect.
FD1514	FastDigest NmuCI	Tsp45I	^GTSAC CASTG^ GTSAC	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect.
FD1524	FastDigest PdiI	MroNI, NaeI, NgoMIV	GCC^GGC CGG^CCG GCCGGC	blunt	37° C	65°C, 15 min	Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect.
FD1534	FastDigest PdmI	Asp700I, MroXI, XmnI	GAANN^NNTTC CTTNN^NNAAG GAANNNNTTC	blunt	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: impaired if overlapped.
FD1554	FastDigest PsuI	BstX2I, BstYI, MflI, XhoII	R^GATCY YCTAG^R RGATCY	5’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD1564	FastDigest XmaJI	AspA2I, AvrII, BlnI	C^CTAGG GGATC^C CCTAGG	5’ protruding ends	37° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD1574	FastDigest Hpy8I	Hpy166II	GTN^NAC CAN^NTG GTNNAC	blunt	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: impaired if overlapped.
FD1634	FastDigest OliI	AleI	CACNN^NNGTG GTGNN^NNCAC CACNNNNGTG	blunt	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: blocked if overlapped. EcoBI: blocked if overlapped.
FD1644	FastDigest SatI	Fnu4HI, Fsp4HI, ItaI	GC^NGC CGN^CG GCNGC	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect.
FD1654	FastDigest TauI		GCSG^C C^GSCG GCSGC	3’ protruding ends	55° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD1664	FastDigest FspAI		RTGC^GCAY YACG^CGTR RTGCGCAY	blunt	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked.
FD1703	FastDigest DpnI	MalI	GA^TC CT^AG GATC	blunt	37° C	80°C, 5 min	Dam: does not cut dam- DNA. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD1704	FastDigest DpnI	MalI	GA^TC CT^AG GATC	blunt	37° C	80°C, 5 min	Dam: does not cut dam - DNA. Dcm: no effect. Cpg: no effect. EcoKI: no effect.
FD1714	FastDigest BseJI	BsaBI, Bse8I	GATNN^NNATC CTANN^NNTAG GATNNNNATC	blunt	37° C	No (column purification)	Dam: blocked if overlapped. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD1724	FastDigest AasI	DrdI, DseDI	GACNNNN^NNGTC CTGNN^NNNNCAG GACNNNNNNGTC	3’ protruding ends	37° C	80°C, 10 min	Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect.
FD1734	FastDigest HpyF10VI	BstMWI, MwoI	GCNNNNN^NNGC CGNN^NNNNNCG GCNNNNNNNGC	3’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect.
FD1744	FastDigest BveI	Acc36I, BfuAI, BspMI	ACCTGCNNNN^NNNN TGGACGNNNNNNNN^ ACCTGCNNNNNNNN	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: impaired if overlapped.
FD1754	FastDigest PfoI		T^CCNGGA AGGNCC^T TCCNGGA	5’ protruding ends	37° C	65°C, 5 min	Dam: impaired if overlapped. Dcm: blocked if overlapped. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect.
FD1764	FastDigest FspBI	BfaI, MaeI, XspI	C^TAG GAT^C CTAG	5’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD1784	FastDigest PfeI	TfiI	G^AWTC CTWA^G GAWTC	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. EcoKI: no effect.
FD1794	FastDigest SsiI	AciI, BspACI	C^CGC GGC^G CCGC	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD1814	FastDigest FaqI	BslFI, BsmFI	GGGACNNNNNNNNNN^NNNN CCCTGNNNNNNNNNNNNNN^ GGGACNNNNNNNNNNNNNN	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect.
FD1824	FastDigest SfiI		GGCCNNNN^NGGCC CCGGN^NNNNCCGG GGCCNNNNNGGCC	3’ protruding ends	50° C	No (column purification)	Dam: no effect. Dcm: impaired if overlapped. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect.
FD1834	FastDigest Hin1II	CviAII, FaeI, FatI, Hsp92II, NlaIII	CATG^ ^GTAC CATG	3’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD1854	FastDigest HhaI	AspLEI, BstHHI, CfoI, HinP1I, HspAI	GCG^C C^GCG GCGC	3’ protruding ends	37° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD1884	FastDigest HpyF3I	BstDEI, DdeI	C^TNAG GANT^C CTNAG	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD1894	FastDigest SgsI	AscI, PalAI	GG^CGCGCC CCGCGC^GG GGCGCGCC	5’ protruding ends	37° C	65°C, 20 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD1904	FastDigest CseI	HgaI	GACGCNNNNN^NNNNN CTGCGNNNNNNNNNN^ GACGCNNNNNNNNNN	5’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect.
FD1934	FastDigest LguI	BspQI, PciSI, SapI	GCTCTTCN^NNN CGAGAAGNNNN^ CTCTTCNNNN	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD1954	FastDigest AjuI		NNNNN^NNNNNNNGAANNNNNNNTTGGNNNNNNNNNNN^ ^NNNNNNNNNNNNCTTNNNNNNNAACCNNNNNN^NNNNN NNNNNNNNNNNNGAANNNNNNNTTGGNNNNNNNNNNN	3’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD1974	FastDigest Ppu21I	BsaAI, BstBAI	YAC^GTR RTG^CAY YACGTR	blunt	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked if overlapped.
FD2004	FastDigest RseI	MslI, SmiMI	CAYNN^NNRTG GTRNN^NNYAC CAYNNNNRTG	blunt	37° C	65°C, 20 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: blocked if overlapped.
FD2024	FastDigest MreI		CG^CCGGCG GCGGCC^GC CGCCGGCG	3’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD2044	FastDigest BspOI	AsuNHI, BmtI	GCTAG^C C^GATCG GCTAGC	3’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
FD2064	FastDigest AanI	PsiI	TTA^TAA AAT^ATT TTATAA	blunt	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoBI: no effect.
FD2074	FastDigest Lsp1109I	BbvI, BstV1I	GCAGCNNNNNNNN^NNNN CGTCGNNNNNNNNNNNN^ GCAGCNNNNNNNNNNNN	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD2084	FastDigest MauBI		CG^CGCGCG GCGCGC^GC CGCGCGCG	3’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD2094	FastDigest SfaAI	AsiSI, RgaI, SgfI	GCGAT^CGC CGC^TAGCG GCGATCGC	3’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD2104	FastDigest TscAI	TspRI	NNCASTGNN^ ^NNGTSACNN NNCASTGNN	3’ protruding ends	65° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: no effect.
FD2114	FastDigest CsiI	MabI, SexAI	A^CCWGGT TGGWCC^A ACCWGGT	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect.
FD2124	FastDigest BmsI	BhaI, BscAI, BscUI, BsmNI, BspST5I, Bst19I, CjeP338II, LweI, Mva1312II, PhaI, SfaNI	GCATCNNNNN^NNNN CGTAGNNNNNNNNN^ GCATCNNNNNNNNN	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect.
FD2134	FastDigest PteI	BsePIm BssHII	G^CGCGC CGCGC^G GCGCGC	5’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD2144	FastDigest FokI	BstF5I, BtsCI, FokI	GGATGNNNNNNNNN^NNNN CCTACNNNNNNNNNNNNN^ GGATGNNNNNNNNNNNNN	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
FD2154	FastDigest RruI	BtuMI, NruI	TCG^CGA AGC^GCT TCGCGA	blunt	37° C	No (column purification)	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
FD2164	FastDigest KflI	Sse1825I, SanDI	GG^GWCCC CCCWG^GG GGGWCCC	5’ protruding ends	37° C	No (column purification)	Dam: no effect. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect.
FD2174	FastDigest SaqAI	MseI, Tru1I, Tru9I	T^TAA AAT^T TTAA	5’ protruding ends	37° C	65°C, 5 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoBI: no effect.
FD2184	FastDigest BfoI	AccB2I, Bme142I, BsmHI, Bsp143II, Bst16I, Bst1473II, BstH2I, Btu34II, HaeII, HinHI, LpnI, NgoAI, NgoBI, NgoCI, NgoGI, NgoJI, NgoMI, NgoWI	RGCGC^Y Y^CGCGR RGCGCY	3’ protruding ends	37° C	65°C, 10 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect.
FD2204	FastDigest PacI		TTAAT^TAA AAT^TAATT TTAATTAA	3’ protruding ends	37° C	65°C, 10 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoBI: no effect.
FD2224	FastDigest PspFI		CCCAG^C G^GGTCG CCCAGC	3’ protruding ends	37° C	80°C, 5 min	Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect.