Proliferating cells labeled in vivo with the Click-iT® EdU assay.

EdU was administered to mice intraperitoneally 2 hours before sacrifice. After intestinal tissues were formalin fixed and paraffin embedded, EdU was labeled with the Click-iT® reaction from the Click-iT® EdU Alexa Fluor® 488 Imaging Assay (Cat. no. C10337), using approximately 250 µL of reaction cocktail per slide. The tissue sections were then washed and treated with mounting media containing DAPI, EdU-positive cells are labeled green, nuclei are stained blue and red autofluorescence was enhanced for image contrast.

EdU was administered to mice intraperitoneally 2 hours before sacrifice. After intestinal tissues were formalin fixed and paraffin embedded, EdU was labeled with the Click-iT® reaction from the Click-iT® EdU Alexa Fluor® 488 Imaging Assay (Cat. no. C10337), using approximately 250 µL of reaction cocktail per slide. The tissue sections were then washed and treated with mounting media containing DAPI, EdU-positive cells are labeled green, nuclei are stained blue and red autofluorescence was enhanced for image contrast.

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