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NGM Detect PCR Amplification Kit
Introducing the NGM Detect PCR Amplification Kit
The Applied Biosystems NGM Detect PCR Amplification Kit is a new, powerful forensic DNA analysis solution that offers superior sensitivity to maximize recovery of data from casework samples. Powered by 6-dye Chemistry, the new NGM Detect kit builds on the well established NGM SElect format, with new primer sequences that enable optimal marker positioning developed specifically for users who require loci based on ENFSI and EDNAP requirements. Together, the two kits offer a powerful combination to gather maximal information from challenging samples.
This new kit enables you to detect more with:
- Increased database searches due to minimal size of markers with highest discriminatory power (such as SE33)
- Dual amplification compatibility between NGM Detect and NGM SElect kits—same loci, complementary marker positions
- High sensitivity due to the ability to add additional DNA volume to the reaction (15 μL vs. 10 μL)
- Additional Y marker to enable gender confirmation
- Integrated quality control (IQC) system to verify reaction setup and assess for sample inhibition
Ordering Information
Superior performance with degraded samples
Fig: Superior performance with degraded samples. The NGM Detect kit shows higher recovery of alleles with an aged and degraded casework sample.
Feature | NGM Detect PCR Amplification Kit |
PCR cycling time | <60 min |
Total number of markers | 18 (16 autosomal STRs plus Amelogenin & Y indel) |
Number of mini-STRs (whole marker range <230 bp) | 7 |
Total reaction volume | 25 µL |
Sample input volume | 15 µL |
Size of SE33 amplicon | Minimum: 184, Maximum: 333 |
Sex identification redundancy | Amelogenin and Y indel |
Number of dyes | 6 |
Dual amplification capabilities | Can be partnered with the NGM SElect kit for dual amplification purposes |
PCR verification system | IQC-2 markers, one low molecular weight (LMW) and one high molecular weight (HMW) flanking STR and markers for sex confirmation |
Need help bringing products online?
Look no further than our Human Identification Professional Services (HPS) team. Since 2007, the team has completed over 400 successful validation projects worldwide.
Over 20 technical support specialists, averaging 8 years of real-world forensic experience each, provide customers with in-depth training and support on our instruments, chemistries, and software.
Resources & FAQs
Q: Do the primers in the NGM Detect kit have the same sequences as those in the previous AmpFlSTR kits?
A: All but one of the STR primers in the NGM Detect kit are unique relative to previous AmpFlSTR kits and the GlobalFiler product family.
This enables results generated with the NGM Detect kit to be confirmed with the NGM SElect kit, and vice versa, by virtue of their unique primer sequences, giving you confidence that results you are reporting are truly representative of the sample tested.
We conducted extensive population studies (N = 1,165 across four population groups) to demonstrate concordance with existing datasets generated with earlier kits; 99.7% concordance was observed, with discordant results attributed to drop-out in the kit used to originally generate the data, with the NGM Detect kit recovering the missing allele.
Q: What do I need in order to evaluate the NGM Detect kit?
A: In summary, the following are required to run the NGM Detect kit:
- An Applied Biosystems 3500 Genetic Analyzer or a 6-dye–enabled 3130 Genetic Analyzer (with data collection software v4.
- GeneMapper ID-X v1.5.2 or above (versions v1.2 to 1.5 can be used to analyze NGM Detect data, but some data quality assessment features are not enabled in these earlier versions)
- Applied Biosystems DS37 Matrix Standard (Dye Set J6-T)
- NGM Detect panels and bins for GeneMapper ID-X Software
- GS600LIZ size standard
See the user guide for the NGM Detect kit for the full workflow requirements.
Q: Why is the matrix standard needed for the NGM Detect kit different from other Applied Biosystems 6-dye chemistries?
A: The NGM Detect kit contains primers that are labeled with a set of dyes different from those used in other Applied Biosystems 6-dye chemistry products. This means a different matrix is required for spectral calibration. This matrix standard is the DS-37 Matrix Standard (Dye Set J6-T). Dye Set J6-T is different from Dye Set J6 (DS-36 Matrix Standard)—it contains the TED dye instead of the NED dye.
Q: What data analysis software should I use for analyzing data from the NGM Detect kit?
A: To perform analysis of NGM Detect kit data, GeneMapper ID-X v1.5.2 software is required.
- Prior versions of GeneMapper ID-X (v1.2 and above) can be used to analyze data generated using the NGM Detect kit. However, some data quality assessment tools are not fully functional on versions prior to 1.5.2:
- Versions prior to 1.4—GeneMapper ID-X v1.4 was developed specifically to handle the additional Y marker information that was first introduced in the GlobalFiler kits. Samples analyzed with versions prior to 1.4 will show a red flag for the CGQ if the Y-indel peak is missing, such as in the case of a female DNA sample.
- Versions prior to 1.5.2—GeneMapper ID-X v1.5.2 was developed specifically to handle the IQC system that has been introduced in the NGM Detect kit. When the sample type is set as “Negative Control”, samples analyzed using versions prior to 1.5.2 will be assigned red CGQ flags because the presence of the IQC peaks triggers the Allele Number Rule for this sample type (no peaks are expected in a valid negative control sample).
Q: How do I obtain GeneMapper ID-X v1.5.2 software?
A: GeneMapper ID-X v1.5 must be installed first. To upgrade to version 1.5.2, you can download and install a patch or contact your local Human Identification representative.
A passion for delivering products you can trust
“My assessment has shown that there is a good correlation between the production of consumables at the Thermo Fisher Scientific sites in Warrington and the requirements stipulated in the ISO 18385 standard.”
—Kevin Sullivan, PhD, director at Principal Forensic Services
- We are proud to declare that our manufacturing facilities are ISO 18385–compliant and delivering high-quality forensic DNA–grade products.
- We are dedicated to producing products that meet high industry standards, so you can get the answers you trust.
- We achieve this though our three quality pillars:
For Research, Forensic, or Paternity Use Only. Not for use in diagnostic procedures.
For licensing and limited use restrictions, visit thermofisher.com/HIDlicensing.