The iBlot 3 Western Blot Transfer System is our next-generation dry transfer device. With several new features, including two independently controlled transfer stations, faster transfer protocols, and higher throughput, it offers even more convenience than the original iBlot and iBlot 2 devices. The iBlot 3 also delivers exceptional performance with simple, high efficiency western blotting transfer in as few as three minutes.
Upgrade to iBlot 3
The next generation in performance and convenience
Comparison of the iBlot 2 and iBlot 3 Devices
iBlot 3 device | iBlot 2 device | |
| Transfer time | 3-10 min | 5-10 min |
| Blot throughput | 1–4 mini gels 1–2 midi gels | 1–2 mini gels 1 midi gel |
| Preassembled transfer stack tray inserts right into the device | ✔ | ✔ |
| Touchscreen | ✔ | ✔ |
| Built-in cooling | ✔ | – |
| Flexibility—can run multiple programs simultaneously | ✔ | – |
| Extended warranty | ✔ | – |
Maximum productivity
With the iBlot 3 you can perform reliable, high-quality transfers faster than ever—in as few as three minutes. In addition, two independently controlled transfer stations allow you to share the device with your coworker, simultaneously running a total of four mini gels or two midi gels with two different programs.
Consistent, reproducible results
The preassembled single-use consumables and ultrafast transfer methods eliminate variability—helping ensure consistent and repeatable results compared with classical methods. In addition, the all-new robust design with built-in cooling reduces heat buildup and enables improved run-to-run consistency.
The iBlot 3 device delivers consistent results. A serial dilution of HEK293 lysate (lysed in Thermo Scientific RIPA buffer) was loaded onto four Invitrogen Bolt 4–12% Bis-Tris Plus gels. Protein loads were (left to right, in μg): 10, 5, 2.5, 1.25, 0.625, 0.312, 0.156, 0.078, 0.039, 0.020, and 0.010. Invitrogen iBright Prestained Protein Ladder (2 μL) was loaded into the last lane. MES-SDS running buffer was used for gel electrophoresis. Proteins were transferred using the iBlot 3 Western Blot Transfer Device and iBlot 3 Transfer Stacks, Midi, NC using the broad range (30–250 kDa) preprogrammed transfer method (25 V, 6 min, low cooling). Blots were then blocked with Pierce Clear Milk Blocking Buffer and were then probed using primary antibodies raised against epidermal growth factor receptor (EGFR), calreticulin, and p23 in clear milk for 12 hours. Secondary antibodies GAR-Invitrogen Alexa Fluor Plus 800 dye, GAM-Invitrogen Alexa Fluor Plus 488 dye, and GAChk-Invitrogen Alexa Fluor 546 dye were prepared in TBST and added to the blots for 2 hours. Immunoprocessing was completed on the Bandmate Automated Western Blot Processor. Imaging was completed on the iBright FL1500 Imaging System. Note: GAChk = goat anti-chicken.
Better Transfer Efficiency
With the iBlot 3, you can achieve as good or better protein transfer efficiency compared with wet tank transfer and other rapid transfer methods. This is because the new design offers more optimization options than ever including built-in cooling. Temperature control also allows you to perform consecutive runs without waiting for the system to cool between runs.
The iBlot 3 system demonstrates better transfer efficiency. (A) Hsp70: Invitrogen NuPAGE 4–12% Bis-Tris mini gels were loaded with 20, 15, 10, 5, 2.5, 1.25, 0.625, and 0.312 μg of A431 lysate. Immunoprocessing was completed using the Invitrogen Bandmate Automated Western Blot Processor. Blots were incubated with Thermo Scientific Pierce Clear Milk Blocking Buffer for 30 minutes. Hsp70 primary antibody (1:1,000 in clear milk) was added to blots and incubated for 12 hours at RT. GAM-HRP secondary antibody (1:120,000 in TBST) was added to blots and incubated for 2 hours. Thermo Scientific SuperSignal West Dura substrate was used for detection. Invitrogen iBright FL1500 Imaging System was used for image capture. (B) 4EBP1: Invitrogen Novex 16% Tricine mini gels were loaded with 10, 5, 2.5, 1.25, 0.625, 0.312, 0.156, and 0.078 μg of A431 lysate. Immunoprocessing was completed using the Bandmate Automated Western Blot Processor. Blots were incubated with Pierce Clear Milk Blocking Buffer for 30 minutes. 4EBP1 primary antibody (1:1,000 in clear milk) was added to blots and incubated for 12 hours at RT. GAR-HRP secondary antibody (1:175,000 in TBST) was added to blots and incubated for 2 hours. SuperSignal West Dura substrate was used for detection. The iBright FL1500 instrument was used for image capture.
Contact your local sales representative to take advantage of special pricing offered for system upgrades.
Terms & Conditions
North America: This promotion is open to customers in the US and Canada. Discount will apply to orders received by Life Technologies no later than August 15, 2023 or until promotional supplies are depleted, whichever comes first. Customer can use the discount only once. Discount only applies to a maximum of 1 device per customer. Discount applies to list price in effect at the time order is received by Life Technologies. Cannot be combined with other discounts or promotions. Offer void where prohibited, licensed or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.
International: Discount will apply to qualifying orders received by Life Technologies no later than August 15, 2023 or until promotional supplies are depleted, whichever comes first. Customer can use the discount only once. Discount only applies to a maximum of 1 device per customer. Discount applies to list price in effect at the time order is received by Life Technologies. Cannot be combined with other discounts or promotions. Offer void in where prohibited, licensed or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.