Target multiple respiratory pathogens on a single panel

 

In order to effectively study outbreaks and determine disease etiology where pathogens are similar, analytically sensitive panels are needed to support laboratory research studies. Applied Biosystems TrueMark Respiratory Research Panels are analytically sensitive, real-time PCR syndromic panels for the analysis of a wide range of respiratory pathogens including viruses. bacteria, and fungi. Our solutions accurately detect many pathogenic organism targets, thereby helping enable your lab to deliver more informative results while achieving greater efficiency. These solutions also solve many of the challenges associated with the use of immunoassays, culture-based, and even other molecular methods available in research assays today.

Broad pathogen detection—Our solutions detect a broad range of viral, bacterial, and fungal targets in one panel.

Flexible input types—Multiple sample input types can be used, including nasopharyngeal (NP) swabs, bronchoalveolar lavage (BAL), and nasal aspirate

Highly accurate—Enable maximum strain coverage while minimizing off-target cross-reactivity

Scalable and efficient—Our variety of real-time PCR assay formats enable you to process tens to hundreds of samples per day on a single instrument

For Research Use Only. Not for use in diagnostic procedures.


Expand your respiratory research with real-time PCR
 

Polymicrobial detection

Real-time PCR can detect slow-growing or difficult-to-culture microorganisms and can be used when traditional methods lead to ambiguous results. Our TrueMark Respiratory Research Panel solutions are available in OpenArray Plates, TaqMan Array Cards, and TaqMan Array Plates, and can be used with multiple sample input types, such as nasopharyngeal (NP) swabs, bronchoalveolar lavage (BAL), and nasal aspirate.

Utilizing TaqMan Assays enable rapid, accurate detection and categorization of multiple microorganisms involved in respiratory tract microbiome composition and dynamics. Each TaqMan Array Card can detect up to 41 pathogens, while the OpenArray (OA) Plates can detect up to 33 pathogens with the ability to run up to 24 samples per plate.  Both formats are available in two different versions—with or without SARS-CoV 1 & 2 targets—depending on your research needs.

TrueMark respiratory tract infection panel details

43
Targets available

12
Bacteria

1
Fungus

30
Viruses

 


Viruses 
Adenovirus
Bocavirus
Coronavirus 229E
Coronavirus HKU1
Coronavirus NL63
Coronavirus OC43
Enterovirus (Pan)
Enterovirus D68
Herpesvirus 6 (HHV6)
HHV3 (varicella zoster virus)
HHV4 (Epstein-Barr virus)
HHV5 (cytomegalovirus)
Metapneumovirus (hMPV)
Influenza A (Pan)
Influenza A/H1-2009
Influenza A/H3
Influenza B
Measles virus
MERS-CoV
Mumps virus
Parainfluenza virus 1
Parainfluenza virus 2
Parainfluenza virus 3
Parainfluenza virus 4
Parechovirus
Respiratory Syncytial Virus A (RSV A)
Rhinovirus
Respiratory Syncytial Virus B (RSV B)
SARS-CoV (SARS)**
SARS-CoV-2**

Fungus 
P. jirovecii


Bacteria
Bordetella (Pan)
Bordetella pertussis
Bordatella holmesii*
Chlamydophila pneumoniae
Coxiella burnetta*
Haemophilus influenzae
Klebsiella pneumoniae complex
Legionella pneumophila
Moraxella catarrhalis
Mycoplasma pneumoniae
Staphylococcus aureus
Streptococcus pneumoniae


*Only available on TaqMan™ Array Respiratory Tract Microbiota Comprehensive Card
**Only available on TrueMark™ Respiratory Panel 2.0, TaqMan™ Array card

For Research Use Only. Not for use in diagnostic procedures.
 

Pathogen Type

TaqMan Card

TaqMan Card 2.0

Bacterial

S. aureus

S. aureus

K. pneumoniae

K. pneumoniae

C. pneumoniae

C. pneumoniae

L. pneumophila

L. pneumophila

C. burnetii

 

S. pneumoniae

S. pneumoniae

M. pneumoniae

M. pneumoniae

B. holmesii

 

M. catarrhalis

M. catarrhalis

B. pertussis

B. pertussis

Bordetella

Bordetella

H. influenzae

H. influenzae

B.atrophaeus

B. atrophaeus

Fungal

P. jirovecii

P. jirovecii

Viral

HHV6

HHV6

EV_pan

EV_pan

SARS_CoV

SARS_CoV

hPIV1

hPIV1

HHV5

HHV5

MERS_CoV

MERS_CoV

CoV_OC43

CoV_OC43

HHV3

HHV3

Mumps

Mumps

EV_D68

EV_D68

hPIV3

hPIV3

CoV_229E

CoV_229E

hPIV2

hPIV2

CoV_NL63

CoV_NL63

CoV_HKU1

CoV_HKU1

HHV4

HHV4

AdV_1of2

AdV_1of2

AdV_2of2

AdV_2of2

HBoV

HBoV

hMPV

hMPV

hPIV4

hPIV4

HPeV

HPeV

Flu_A_H1

Flu_A_H1

Flu_A_H3

Flu_A_H3

Flu_A_pan

Flu_A_pan

Flu_B_pan

Flu_B_pan

Measles

Measles

RSVA

RSVA

RSVB

RSVB

RV_1of2

RV_1of2

RV_2of2

RV_2of2

 

SARS-CoV2S

 

SARS-CoV2N

Control

Xeno

Xeno

RPPH1

RPPH1

18s

18s

Pathogen Type

OpenArray Plate

OpenArray Plate 2.0

Bacterial

S. aureus

S. aureus

K. pneumoniae

K. pneumoniae

C. pneumoniae

C. pneumoniae

L. pneumophila

L. pneumophila

S. pneumoniae

S. pneumoniae

M. pneumoniae

M. pneumoniae

B. pertussis

B. pertussis

Bordetella

Bordetella

H. influenzae

H. influenzae

Viral

HHV6

HHV6

EV_pan

EV_pan

hPIV1

hPIV1

HHV5

HHV5

CoV_OC43

CoV_OC43

HHV3

 

EV_D68

EV_D68

hPIV3

hPIV3

CoV_229E

CoV_229E

hPIV2

hPIV2

CoV_NL63

CoV_NL63

CoV_HKU1

CoV_HKU1

HHV4

HHV4

AdV_1of2

AdV_1of2

AdV_2of2

AdV_2of2

HBoV

 

hMPV

hMPV

hPIV4

hPIV4

Flu_A_H1

Flu_A_H1

Flu_A_H3

Flu_A_H3

Flu_A_pan

Flu_A_pan

Flu_B_pan

Flu_B_pan

RSVA

RSVA

RSVB

RSVB

RV_1of2

RV_1of2

RV_2of2

RV_2of2

 

SARS-CoV2S

 

SARS-CoV2N

Control

IC Xeno

IC Xeno

IC B.atrophaeus

IC B.atrophaeus

IC RNase P

IC RNase P

To download a PDF version of the information above, please visit Pathogen Target Guide for Respiratory Panels.

For Research Use Only. Not for use in diagnostic procedures.

Highly accurate

TaqMan Assays for respiratory pathogens have undergone rigorous bioinformatic analysis to help ensure maximum strain coverage while minimizing off-target cross-reactivity. Each assay has been tested with target and non-target genomic RNA or DNA isolated from target organisms (nucleic acid acquired from ATCC) in our inclusivity panel.

Pathogen

True positive*

False negative**

Concordance

Adenovirus

20

1

95.24%

Coronavirus 229E

2

0

100.00%

Coronavirus HKU1

1

0

100.00%

Coronavirus OC34

1

0

100.00%

Influenza A

4

0

100.00%

Influenza A/H1

6

0

100.00%

Influenza A/H3

16

0

100.00%

Influenza B

16

0

100.00%

hMPV

16

0

94.12%

hPIV1

10

1

90.91%

hPIV2

10

0

100.00%

hPIV3

12

0

100.00%

RSV

6

0

100.00%

RSVA

17

1

94.44%

RSVB

16

1

100.00%

Rhinovirus, enterovirus

27

0

100.00%

Total

180

5

97.30%

* Call from the vendor

** Call not detected when tested on TaqMan Array Card Assay (TAC)

The analytical testing characteristics of the assays on the TaqMan Array Respiratory Tract Microbiota Comprehensive Card were examined by testing with 180 procured samples. The control samples included in this study have application in respiratory disease research and cover 16 key respiratory viruses.

Scalable and flexible workflow

The QuantStudio family of instruments can meet any throughput requirement. For higher throughput laboratories, leverage our OpenArray nanofluidic technology. A complete end-to-end solution is available and includes the Thermo Scientific KingFisher Flex benchtop automated DNA/RNA extraction instrument, convenient extraction and purification kits, and the OpenArray AccuFill System. Combined, these systems can automate sample prep and liquid handling to enable the processing of hundreds to thousands of samples per day.    

Whether you already own an instrument or are considering an expansion to your capabilities, your sample processing and throughput requirements can be addressed through different formats compatible with your instrument (i.e. TaqMan Array Cards, 96- or 384-well plates, or single tube assays) with made-to-stock or custom panels.


Ordering
 

Explore QuantStudio PCR Systems, a flexible and scalable real-time PCR platform.

Order the TaqMan™ Array Respiratory Tract Microbiota Comprehensive Card or TrueMark™ Respiratory Panel 2.0, TaqMan™ Array Card


For Research Use Only. Not for use in diagnostic procedures.
 


Services
 

Analytical validation consulting services for research products

When adopting a new testing method for your research needs, we can assist in enabling the verification of the workflow to help ensure a successful launch. Our analytical validation (AV) consulting services can speed up the launch process by up to 75% and cut costs by up to half. Our expert team will consult to help develop and optimize your research workflow, while providing data analysis support and template documentation to fully maximize your instrument and reagent investment.

Learn more ›


Consulting services offered for enabling the analytical validation cannot be used to validate a clinical diagnostic workflow and can only be used to support ongoing true research efforts.
 



Resources
 

Learn more about utilizing sensitive, syndromic panel–based real-time PCR solutions for the research of respiratory pathogens in your lab.


For Research Use Only. Not for use in diagnostic procedures.
 

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