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The Attune Xenith Flow Cytometer is set to be released in the US, Canada, and Europe* by the end of 2024. All other countries will be available in 2025.
The Invitrogen Attune Xenith™ Flow Cytometer is a reliable solution designed to help meet the rigorous demands of researchers. Leveraging our legacy core acoustic focusing technology, this innovative instrument combines acoustic-assisted hydrodynamic focusing with advanced spectral capabilities. With the Attune Xenith instrument, you can evaluate more parameters faster and with high sensitivity. Get data you can trust from a system that won’t slow you down.
Experience the power of the Attune Xenith firsthand—request your personalized demo.
Our Attune Flow Cytometers offer faster sample processing, surpassing traditional cytometers, due to acoustic-assisted hydrodynamic focusing (see figure below). The new Attune Xenith Flow Cytometer integrates acoustic focusing with advanced spectral flow capabilities, offering outstanding value. With expanded experimental capabilities and the ability to accommodate a wide range of cell types, the acoustic focusing technology helps ensure exceptional performance. Additionally, the advanced spectral flow capabilities enable simultaneous detection of a larger number of parameters, enhancing sensitivity, improved resolution, and increased flexibility in panel design while helping streamline workflows.
Figure 1. Comparison of acoustic and traditional focusing. Unlike traditional hydrodynamic focusing (B), acoustic focusing maintains a narrow core width even at faster sample flow rates (A). This precise alignment ensures that only one cell passes through the laser at a time, eliminating the risk of overlapping signals and enhancing data accuracy.
Experience efficient, high-resolution workflows for spectral and conventional compensation analysis. Now with more colors, faster results, and the ability to evaluate multiple parameters with high sensitivity using minimal sample volume. The Attune Xenith cytometer offers expanded optical capabilities, including UV/NIR, and supports both traditional compensation and spectral unmixing analysis.
Join us for an insightful presentation on the advancements in flow cytometry. As panel complexity increases, there is a greater demand for advanced instrumentation, panel design services, and a wider range of fluorophore offerings. Spectral unmixing has revolutionized panel size and diversity, enabling exploration of uncharted territories and identification of rare cell populations.
A collection of data showcasing the exceptional performance and capabilities of Attune Xenith Flow Cytometer. This section provides valuable insights and inspiration for your own experiments, allowing you to see data generated using the Attune Xenith Flow Cytometer.
Figure 2. Immunophenotyping of mouse tissue digests using a 32-color panel. In this study, we utilized the advanced fluidics and high detection sensitivity of the Attune Xenith Flow Cytometer to conduct broad spectral unmixing immunophenotyping, with a specific focus on the development and maturation of B cells across various tissues. The instrument's clog-resistant design combined with its acoustic focusing technology allowed for rapid data acquisition without compromising the resolution of the analysis. Even at high cell concentrations of 1E7 cells/mL in mouse tissue digests, the Attune Xenith Flow Cytometer demonstrated its capability to deliver accurate and reliable results.
Figure 3. Precision in rare event detection using a 25-color natural killer cell panel. The study utilized high sensitivity and specificity to interrogate natural killer (NK) cell populations, providing crucial insights for detailed research. Immature, mature and terminal NK populations were identified from human PBMCs, and surface marker expression of each of these subpopulations was characterized. The acquisition of rare events was achieved without the need to concentrate samples, while high sample throughput reduced time to results without compromising population resolution. The sensitivity in detection ensured robust data resolution, enabling the differentiation of even rare populations in high complexity panels. View complete data set here.
Figure 4. Exploration of expression profiles of NK cells across activation states. The study utilized the NK cell panel mentioned earlier to investigate the expression of surface markers before and after activation. Human NK cells were incubated with or without IL-2, IL-15, and IL-21 cytokine cocktail for a duration of 48 hours, and the antigen expression was evaluated. The Attune Xenith Flow Cytometer demonstrated strong sensitivity in detecting changes across different stimulation procedures, ensuring reliable and accurate characterization of these rare cell subsets. This highlights the potential of the panel for conducting in-depth analysis and gaining a deeper understanding of NK cell subsets.
Here we provide a series of articles to help you get started designing and executing experiments using spectral flow cytometry. Topics include an overview of the differences between conventional and spectral methods, panel design, control and sample preparation, panel evaluation, and data analysis.
*Europe includes Denmark, France, Germany, Italy, Netherlands, Norway, Poland, Portugal, Spain, Sweden, Switzerland, United Kingdom. United States and Canada.
For Research Use Only. Not for use in diagnostic procedures.