TaqMan hPSC Scorecard Panel | Thermo Fisher Scientific

Quantitative analysis of trilineage potential of your PSCs

The Applied Biosystems TaqMan hPSC Scorecard Panel assesses pluripotency and trilineage differentiation potential using real-time qPCR assays and intuitive data analysis software. The hPSC Scorecard assay was developed in collaboration with Alex Meissner, and follows from his landmark publication (Cell 2011 144:439–452). The accompanying analysis software gives researchers anywhere in the world the ability to compare the gene expression profiles of their cell lines to a common reference set.

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Why we chose the TaqMan hPSC Scorecard Assay

Hear from Jennifer Moore at RUCDR/Infinite Biologics on the benefits Scorecard has to offer.

Instrument requirements

See which instruments are compatible with the TaqMan hPSC Scorecard Panel and how to set them up.

Data analysis

The TaqMan hPSC Scorecard Panel includes access to free, intuitive web-based analysis software that allows researchers to quantitatively compare the gene expression profile of their sample to that of a reference set.

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What is it?

The TaqMan hPSC Scorecard Panels quantitatively confirms pluripotency (trilineage differentiation potential)
for both embryonic and induced pluripotent stem cell lines. The hPSC Scorecard Panel (available in 96 and 384-well format) contains 94 predefined TaqMan Gene Expression assays (including endogenous controls) dried-down in assay plate wells. The panel of genes was developed in collaboration with Alex Meissner, and based on his landmark publication (Cell 144:439-452 (2011)).

The TaqMan hPSC Scorecard Panel provides:

A quantitative assay for confirming pluripotency and trilineage differentiation potential
Comparison of expression profiles to a reference standard
An easy to use platform with pre-plated assays and dedicated, intuitive analysis software

Overview of protocol:

Test undifferentiated, spontaneous differentiation (EB) or directed (monolayer) differentiation samples
Extract and purify RNA
Generate cDNA
Run gene expression panel on a compatible qRT-PCR instrument
Upload .eds files to the hPSC Scorecard Analysis Software
Analyze data and export results from the convenient cloud-based analysis application