Technical Resources - Media Formulations

11320 - DMEM/F-12

Catalog Number(s)
11320033 , 11320074 , 11320082

Components	Molecular Weight	Concentration (mg/L)	mM
Amino Acids
Glycine	75.0	18.75	0.25
L-Alanine	89.0	4.45	0.049999997
L-Arginine hydrochloride	211.0	147.5	0.69905216
L-Asparagine-H2O	150.0	7.5	0.05
L-Aspartic acid	133.0	6.65	0.05
L-Cysteine hydrochloride-H2O	176.0	17.56	0.09977272
L-Cystine 2HCl	313.0	31.29	0.09996805
L-Glutamic Acid	147.0	7.35	0.05
L-Glutamine	146.0	365.0	2.5
L-Histidine hydrochloride-H2O	210.0	31.48	0.14990476
L-Isoleucine	131.0	54.47	0.41580153
L-Leucine	131.0	59.05	0.45076334
L-Lysine hydrochloride	183.0	91.25	0.4986339
L-Methionine	149.0	17.24	0.11570469
L-Phenylalanine	165.0	35.48	0.2150303
L-Proline	115.0	17.25	0.15
L-Serine	105.0	26.25	0.25
L-Threonine	119.0	53.45	0.44915968
L-Tryptophan	204.0	9.02	0.04421569
L-Tyrosine disodium salt dihydrate	261.0	55.79	0.21375479
L-Valine	117.0	52.85	0.4517094
Vitamins
Biotin	244.0	0.0035	1.4344263E-5
Choline chloride	140.0	8.98	0.06414285
D-Calcium pantothenate	477.0	2.24	0.0046960167
Folic Acid	441.0	2.65	0.0060090707
Niacinamide	122.0	2.02	0.016557377
Pyridoxine hydrochloride	206.0	2.013	0.009771844
Riboflavin	376.0	0.219	5.824468E-4
Thiamine hydrochloride	337.0	2.17	0.0064391694
Vitamin B12	1355.0	0.68	5.0184503E-4
i-Inositol	180.0	12.6	0.07
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.)	111.0	116.6	1.0504504
Cupric sulfate (CuSO4-5H2O)	250.0	0.0013	5.2E-6
Ferric Nitrate (Fe(NO3)3"9H2O)	404.0	0.05	1.2376238E-4
Ferric sulfate (FeSO4-7H2O)	278.0	0.417	0.0015
Magnesium Chloride (anhydrous)	95.0	28.64	0.30147368
Magnesium Sulfate (MgSO4) (anhyd.)	120.0	48.84	0.407
Potassium Chloride (KCl)	75.0	311.8	4.1573334
Sodium Bicarbonate (NaHCO3)	84.0	2438.0	29.02381
Sodium Chloride (NaCl)	58.0	6995.5	120.61207
Sodium Phosphate dibasic (Na2HPO4) anhydrous	142.0	71.02	0.50014085
Sodium Phosphate monobasic (NaH2PO4-H2O)	138.0	62.5	0.45289856
Zinc sulfate (ZnSO4-7H2O)	288.0	0.432	0.0015
Other Components
D-Glucose (Dextrose)	180.0	3151.0	17.505556
Hypoxanthine Na	159.0	2.39	0.015031448
Linoleic Acid	280.0	0.042	1.4999999E-4
Lipoic Acid	206.0	0.105	5.097087E-4
Phenol Red	376.4	8.1	0.021519661
Putrescine 2HCl	161.0	0.081	5.031056E-4
Sodium Pyruvate	110.0	55.0	0.5
Thymidine	242.0	0.365	0.0015082645

Reference:

1. Dulbecco, R. and Freeman, G., (1959) Plaque formation by the polyoma virus. Virology 8:396.

2. Ham, R.G., (1965) Clonal growth of mammalian cells in a chemically defined synthetic medium. Proc. Natl. Acad. Sci., 53:288.

3. Morton, H.J. (1970) A survey of commercially available tissue culture media. In Vitro 6(2):89.

4. Smith, J.D., Freeman, G., Vogt, M., et al., (1960) The nucleic acid of polyoma virus. Virology 12:185.

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