Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Flow cytometry intrinsically facilitates the analysis of the effects of cell treatment in heterogeneous cell populations. Antibodies are tested for staining following treatment of cells with a known stimulant. This representative image demonstrates the type of data that was used in the verification of specificity for this antibody. Legend: Normal human peripheral blood cells were unstimulated (blue histogram) or stimulated for 3 days with Con A and LPS (purple histogram). Cells were stained with Fixable Viability Dye eFluor 780, CD4 (clone SK3) and CD25 (clone CD25-4E3). Viable cells in the CD4+ gate were used for analysis. As expected based on known expression patterns, there is a significant increase in CD25 antibody staining in CD4+ T cells after stimulation.