Simultaneous detection of expression of five genes in a whole mount Drosophila embryo by fluorescence in situ hybridization (FISH) with five RNA probes

Simultaneous detection of expression of five genes in a whole-mount Drosophila embryo by fluorescence in situ hybridization (FISH) with five RNA probes. Red: sog labeled using aminoallyl UTP (Cat. No. A21663, A32765) and Alexa Fluor® 647 succinimidyl ester (Cat. No. A20006, A20106). Green: ind labeled with DNP, followed by rabbit anti–dinitrophenyl-KLH IgG antibody (Cat. No. A6430) prelabeled with the Zenon® Alexa Fluor® 555 Rabbit IgG Labeling Kit (Cat. No. Z25305). Blue: en labeled with biotin and detected with HRP–streptavidin and Alexa Fluor® 405 tyramide (TSA™ Kit #39, T30952). Yellow: wg labeled with digoxigenin and detected with sheep anti–digoxigenin IgG antibody and Alexa Fluor® 594 Donkey Anti–Sheep IgG antibody (Cat. No. A11016). Magenta: msh labeled with fluorescein and detected with mouse anti–fluorescein/Oregon Green® IgG2a antibody (Cat. No. A6421) and Alexa Fluor® 488 Goat Anti–Mouse IgG antibody (Cat. No. A11001, A11029). Image contributed by Dave Kosman and Ethan Bier, University of California, San Diego.

Simultaneous detection of expression of five genes in a whole-mount Drosophila embryo by fluorescence <i>in situ</i> hybridization (FISH) with five RNA probes. Red: <i>sog</i> labeled using aminoallyl UTP (Cat. No. A21663, A32765) and Alexa Fluor® 647 succinimidyl ester (Cat. No. A20006, A20106). Green: <i>ind</i> labeled with DNP, followed by rabbit anti–dinitrophenyl-KLH IgG antibody (Cat. No. A6430) prelabeled with the Zenon® Alexa Fluor® 555 Rabbit IgG Labeling Kit (Cat. No. Z25305). Blue: <i>en</i> labeled with biotin and detected with HRP–streptavidin and Alexa Fluor® 405 tyramide (TSA™ Kit #39, T30952). Yellow: <i>wg</i> labeled with digoxigenin and detected with sheep anti–digoxigenin IgG antibody and Alexa Fluor® 594 Donkey Anti–Sheep IgG antibody (Cat. No. A11016). Magenta: <i>msh</i> labeled with fluorescein and detected with mouse anti–fluorescein/Oregon Green® IgG<sub>2a</sub> antibody (Cat. No. A6421) and Alexa Fluor® 488 Goat Anti–Mouse IgG antibody (Cat. No. A11001, A11029). Image contributed by Dave Kosman and Ethan Bier, University of California, San Diego.

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