Automated Protein Structure Analysis Using DIA Based Hydrogen Deuterium Exchange Mass Spectrometry

Hydrogen/deuterium exchange coupled to mass spectrometry (HX-MS) is a valuable technique for analyzing the structural features and dynamic properties of individual proteins and large protein complexes. HX-MS can be used to study a wide range of structural properties including protein folding, ligand binding and allostery, and epitope mapping in the development of therapeutic antibodies. However, HX-MS is not routinely used in protein structure analysis because of data analysis challenges that limit its throughput and scope.  

In this GEN webinar, Dr. David Schriemer (University of Calgary) will present an innovative approach for applying HX-MS to protein analysis in biotherapeutics development. He will demonstrate a data independent acquisition-based HX-MS workflow that significantly reduces analysis times, enables the study of larger datasets, and paves the way for a standardized strategy for protein structure analysis. Dr. Schriemer will show how HX-MS can rapidly characterize drug-binding interactions using a 500-kDa mammalian kinase using affinity pulldowns.  

Key takeaways include:

• Discovering the latest MS advances that benefit HX-MS technology.
• Learning about the implementation of data-independent acquisition for HX-MS.
• Learning how to use HX-MS to study drug binding interactions and higher-order protein structure dynamics.
• Understanding the data mining approach used to confirm peptide ID and authenticate, or even correct, MS1-based deuteration calculations.

About the presenter