Fluorescent Secondary Antibodies

Fluorescent-dye conjugated secondary antibodies help provide a tool for identifying proteins in many applications including fluorescent cell imaging, western blotting, immunohistochemistry, and more. The advantages of using a fluorescently labeled secondary antibody include brighter signal, multiplexing capabilities, and ease of use. Browse our portfolio of Alexa Fluor secondary antibodies, classic secondaries (such as FITC and TRITC), and our selection of enzyme and biotin-labeled secondaries.

Available fluorescent secondary antibody conjugates

Alexa Fluor conjugates	Alexa Fluor Plus conjugates	DyLight conjugates	Classic fluorophore conjugates
Alexa Fluor 350 Alexa Fluor 405 Alexa Fluor 430 Alexa Fluor 488 Alexa Fluor 514 Alexa Fluor 532 Alexa Fluor 546 Alexa Fluor 555 Alexa Fluor 568 Alexa Fluor 594 Alexa Fluor 633 Alexa Fluor 635 Alexa Fluor 647 Alexa Fluor 660 Alexa Fluor 680 Alexa Fluor 700 Alexa Fluor 750 Alexa Fluor 790	Alexa Fluor Plus 405 Alexa Fluor Plus 488 Alexa Fluor Plus 555 Alexa Fluor Plus 594 Alexa Fluor Plus 647 Alexa Fluor Plus 680 Alexa Fluor Plus 800	DyLight 350 DyLight 405 DyLight 488 DyLight 550 DyLight 594 DyLight 633 DyLight 650 DyLight 680 DyLight 755 DyLight 800	APC Cy3 Cy5 FITC PE Pacific dyes Qdots TRITC Rhodamine R-PE Texas Red & Texas Red-X

Fluorescent secondary antibody selection guide

	Classic fluorophore conjugates	Alexa Fluor conjugates	Alexa Fluor Plus conjugates
Use	For detection of high-abundance targets For use with easy to obtain samples.	For detection of medium-to-high- abundance targets For use in most research areas	For detection of low-abundance targets For use with hard to obtain samples
Applications	Cell imaging Immunohistochemistry (IHC) Immunocytochemistry (ICC) Immunofluorescence (IF) Multiplex, fluorescent western blotting (WB)
Signal-to-noise ratio	Low	Medium	High
Photostability	Varying levels of photostability, depending on dye	Photostable	Photostable
Specificity	Various options available	Various options available	High specific, low cross-reactivity (highly cross-adsorbed)
Sensitivity and brightness	Lower sensitivity and brightness compared to leading Alexa Fluor conjugates	Widely recognized standard for brightness and sensitivity	Improved sensitivity and brightness over leading Alexa Fluor conjugates in IF/ICC imaging and fluorescent western blotting
Range of linear detection	Low	Medium	High