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Traditional feeder-free stem cell media were developed over 10 years ago. A lot has changed since then, so why hasn’t your media?

Traditional media formulations for the culture of pluripotent stem cells (PSCs) were originally designed over a decade ago when the most important questions researchers were asking were about how can they reproducibly derive and maintain these cells. Over the course of the last ten years, the use of PSCs has expanded exponentially to more complex and intricate applications, including single-cell passaging and gene editing. Using the original culture media, researchers have had difficulty keeping up with the technological advances of the field and frequently experience the challenges below.

Today’s top stem cell challenges:

  • Aberrant differentiation due to unstable growth factors
  • Slow and variable recovery rates post-passaging
  • Poor cell survival following single cell dissociation
  • Poor cell survival following cryopreservation
  • Challenging workflow schemes required to transition from richer media systems
  • Challenging positive selection and propagation of iPSCs following reprogramming due to small size of colonies
  • Requirement of daily feeding and maintenance upon addition of PSC Medium
  • Poor reprogramming efficiency for traditional media that include BSA
  • Poor cell survival post gene editing
  • Aberrant differentiation upon expansion post gene editing
  • Poor clonal cell survival post gene editing
  • Aberrant differentiation upon expansion of PSCs
  • Incompatibility with downstream differentiation workflows
  • Inconsistent CRISPR editing in stem cells

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