Having difficulties with your experiment?

We are dedicated to your success. Get back on track. View our expert recommendations for commonly encountered problem scenarios.

View the relevant questions below:

 

Beginning your experiment?
Visit our

Getting Started page

 

Your MembranePro™ Functional Protein Expression Kit/Expi293™ MembranePro™ Expression System does not include a positive control. How do I tell if MembranePro™ particles were formed?

You can visually tell if MembranePro™ particles formed via a pellet at the bottom of the tube following precipitation. You can also test the function of your MembranePro™ particles via receptor-ligand binding studies. 

Can I use a different expression vector with the MembranePro™ Functional Protein Expression Kit or the Expi293™ MembranePro™ Expression System?

While other expression vectors and promoters can be tested, we recommend using the pEF6 V5-His TOPO® TA expression vector for optimal yields, as the performance of these kits has been optimized for use with this expression vector containing the EF-1α promoter. 

I had a problem growing up the 293T cells provided in the MembranePro™ Functional Protein Expression Kit. Can I use a different cell line?

While other cell lines can be tested, we recommend using the supplied 293FT cells, as the performance of the kit has been optimized for use with this cell line (i.e., transfection efficiency). 

The Expi293F™ cells supplied with the Expi293™ MembranePro™ Expression System had poor viability upon thawing. Can I use a different cell line?

While other cell lines can be tested, we recommend using the Expi293F™ cells, as the performance of the kit has been optimized for use with this cell line (i.e., transfection efficiency using ExpiFectamine™ Transfection Reagent). 

Need more information? Contact us ›

For Research Use Only. Not for use in diagnostic procedures.