Invitrogen

ACE2 Polyclonal Antibody

ACE2 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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ACE2 Antibody (PA5-47488) in IHC (F)

Immunohistochemical analysis of ACE2 in perfusion fixed frozen sections of mouse kidney. Samples were incubated in ACE2 polyclonal antibody (Product # PA5-47488) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and... View More

ACE2 Antibody in Immunohistochemistry (IHC)

ACE2 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

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Product Details

PA5-47488

Applications

Tested Dilution

Publications

Western Blot (WB)

0.25 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Frozen) (IHC (F))

5-15 µg/mL

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/10^6 cells

ELISA (ELISA)

Assay-Dependent

Immunoprecipitation (IP)

25 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CHO-derived recombinant mouse ACE-2 Gln18-Thr740

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2606505

Product Specific Information

In Western blots, approximately 40% cross-reactivity with recombinant human ACE-2 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Ocular Symptoms Associated with COVID-19 Are Correlated with the Expression Profile of Mouse SARS-CoV-2 Binding Sites.

Viruses

Brechbühl J,Ferreira F,Lopes AC,Corset E,Gilliand N,Broillet MC

PA5-47488 was used in Immunohistochemistry to give new insight into the expression of SARS-CoV-2 binding sites in the mouse eye and propose an interpretation of the human ocular-associated symptoms linked to SARS-CoV-2.

Thu Jan 26 00:00:00 EST 2023

Ocular Symptoms Associated with COVID-19 Are Correlated with the Expression Profile of Mouse SARS-CoV-2 Binding Sites.

Viruses

Brechbühl J,Ferreira F,Lopes AC,Corset E,Gilliand N,Broillet MC

Thu Jan 26 00:00:00 EST 2023

Supporting Cells of the Human Olfactory Epithelium Co-Express the Lipid Scramblase TMEM16F and ACE2 and May Cause Smell Loss by SARS-CoV-2 Spike-Induced Syncytia.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Hernandez-Clavijo A,Gonzalez-Velandia KY,Rangaswamy U,Guarneri G,Boscolo-Rizzo P,Tofanelli M,Gardenal N,Sanges R,Dibattista M,Tirelli G,Menini A

PA5-47488 was used in Immunocytochemistry to investigate if proteins involved in Spike-induced syncytia formation, i.e., ACE2 and TMEM16F, are expressed in the human olfactory epithelium.

Tue Jun 07 00:00:00 EDT 2022

Influenza A virus modulates ACE2 expression and SARS-CoV-2 infectivity in human cardiomyocytes.

iScience

Wu Q,Kumar N,Lafuse WP,Ahumada OS,Saljoughian N,Whetstone E,Zani A,Patton AK,El Refaey M,Webb A,Pietrzak M,Yu L,Kc M,Peeples ME,Ganesan LP,Yount JS,Rajaram MVS

PA5-47488 was used in Immunohistochemistry to determine the impact of IAV infection on SARS-CoV-2 pathogenesis and cardiomyocyte function.

Thu Dec 22 00:00:00 EST 2022

Age-dependent appearance of SARS-CoV-2 entry sites in mouse chemosensory systems reflects COVID-19 anosmia-ageusia symptoms.

Communications biology

Brechbühl J,Lopes AC,Wood D,Bouteiller S,de Vallière A,Verdumo C,Broillet MC

Published figure using ACE2 polyclonal antibody (Product # PA5-47488) in Immunohistochemistry

Thu Jul 15 00:00:00 EDT 2021

Neonatal hyperoxia enhances age-dependent expression of SARS-CoV-2 receptors in mice.

Scientific reports

Yee M,David Cohen E,Haak J,Dylag AM,O'Reilly MA

PA5-47488 was used in Western Blotting to find that hyperoxia enhances the age-dependent expression of SARS-CoV-2 receptors in mice.

Mon Dec 28 00:00:00 EST 2020

Neonatal hyperoxia enhances age-dependent expression of SARS-CoV-2 receptors in mice.

Scientific reports

Yee M,David Cohen E,Haak J,Dylag AM,O'Reilly MA

PA5-47488 was used in Western Blotting to find that hyperoxia enhances the age-dependent expression of SARS-CoV-2 receptors in mice.

Mon Dec 28 00:00:00 EST 2020

Neonatal hyperoxia enhances age-dependent expression of SARS-CoV-2 receptors in mice.

Scientific reports

Yee M,David Cohen E,Haak J,Dylag AM,O'Reilly MA

PA5-47488 was used in Western Blotting to find that hyperoxia enhances the age-dependent expression of SARS-CoV-2 receptors in mice.

Mon Dec 28 00:00:00 EST 2020

Non-neuronal expression of SARS-CoV-2 entry genes in the olfactory system suggests mechanisms underlying COVID-19-associated anosmia.

Science advances

Brann DH,Tsukahara T,Weinreb C,Lipovsek M,Van den Berge K,Gong B,Chance R,Macaulay IC,Chou HJ,Fletcher RB,Das D,Street K,de Bezieux HR,Choi YG,Risso D,Dudoit S,Purdom E,Mill J,Hachem RA,Matsunami H,Logan DW,Goldstein BJ,Grubb MS,Ngai J,Datta SR

PA5-47488 was used in Western Blotting to identify cell types in the olfactory epithelium and olfactory bulb that express SARS-CoV-2 cell entry molecules.

Fri Jul 31 00:00:00 EDT 2020

Neonatal hyperoxia enhances age-dependent expression of SARS-CoV-2 receptors in mice.

bioRxiv : the preprint server for biology

Yee M,Cohen ED,Haak J,Dylag AM,O'Reilly MA

PA5-47488 was used in Western Blot, Immunohistochemistry to make the surprising discovery that expression of Ace2 and Tmprss2 mRNA increases as mice age and is accelerated by exposing mice to neonatal hyperoxia.

Wed Jul 22 00:00:00 EDT 2020

Neonatal hyperoxia enhances age-dependent expression of SARS-CoV-2 receptors in mice.

bioRxiv : the preprint server for biology

Yee M,Cohen ED,Haak J,Dylag AM,O'Reilly MA

Wed Jul 22 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: ACE 2; ACE-related carboxypeptidase; ACEH; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin I converting enzyme 2; Angiotensin-converting enzyme 2; Angiotensin-converting enzyme homolog; Angiotensin-converting enzyme-related carboxypeptidase; anigotensin-converting enzyme-related carboxypeptidase; Metalloprotease MPROT15; peptidyl-dipeptidase A; renal angiotensin-converting enzyme 2

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Gene Aliases: 2010305L05Rik; ACE2; ACEH; UNQ868/PRO1885

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UniProt ID: (Human) Q9BYF1, (Rat) Q5EGZ1, (Mouse) Q8R0I0

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Entrez Gene ID: (Human) 59272, (Rat) 302668, (Mouse) 70008

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Function(s)

virus receptor activity glycoprotein binding endopeptidase activity carboxypeptidase activity metallocarboxypeptidase activity protein binding metallopeptidase activity peptidyl-dipeptidase activity zinc ion binding metal ion binding peptidase activity hydrolase activity metalloprotease

Process(es)

regulation of cytokine production angiotensin maturation angiotensin catabolic process in blood angiotensin-mediated drinking behavior regulation of systemic arterial blood pressure by renin-angiotensin tryptophan transport regulation of vasoconstriction receptor biosynthetic process regulation of cell proliferation regulation of vasodilation viral entry into host cell receptor-mediated virion attachment to host cell regulation of inflammatory response positive regulation of cardiac muscle contraction positive regulation of gap junction assembly regulation of cardiac conduction positive regulation of reactive oxygen species metabolic process proteolysis response to virus negative regulation of smooth muscle cell proliferation maternal process involved in female pregnancy negative regulation of ERK1 and ERK2 cascade

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ACE2 Polyclonal Antibody

ACE2 Antibody (PA5-47488) in IHC (F)

Product Details

PA5-47488

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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