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Miltenyi "MicroBead" Artifacts Detected in T cells after 72 hours of Incubation
The Results
Miltenyi nanoparticles are retained by T cells, after 72hr incubation
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What you’re about to see:
- Confocal, iso-surface and TEM images reveal Miltenyi nanoparticles internalizing in human CD3+ T cells.
- Miltenyi nanoparticles accumulating in human CD3+ T cell vesicles.
What this means:
After 72 hours (see new TEM's below), Miltenyi nanoparticles are not biodegraded. In contrast, Dynabeads® are released and removed from the cells.
Notes: All video imagery is actual footage and unaltered.
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What immunologists say after seeing the videos
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Verify these findings for yourself...
See more data
- Ex vivo isolation protocols differentially affect the phenotype of human CD4+ T cells, (abstract) Bernard et al 2002
Dynabeads® are released & removed from your cells
Positive Cell Isolation
- Learn more about Dynabeads® FlowComp™
Negative Cell Isolation
- Learn more aboutDynabeads® Untouched™
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More TEM images of Miltenyi nanoparticles before and after 24, 48 and 72hr incubation
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24 hrs post isolation: Epon sections of CD3 isolated cells incubated at 37° c showing large multivesicular bodies containing Miltenyi nanoparticles (black specs) at high magnification. | |
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48 hrs post isolation: Epon sections of CD3 isolated cells incubated for at 37° c. High magnification of Miltenyi nanoparticles containing electron dense compartment. | |
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72 hrs post isolation: Epon sections of CD3 cells incubated at 37° c. Miltenyi nanoparticles in different intracellular compartments in two neighboring CD3 positive cells. | |
Avoid Artifacts - Use Dynabeads®
Miltenyi vs. Dynabeads® Comparison Table
Miltenyi nanoparticles | Dynabeads® FlowComp™ | |
| Approx. 50nm | Size | 2.8µM |
| None | Uniformity | Spherical (1-3% variability) |
| No | T-Cell Release? | Yes |
| Yes | Columns Required? | No |
 Yes | Internalized into T-Cells? | No  |
 Yes | Retained in T-Cells after 72hrs? | No  |
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