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HeLa cells were fed 50uM ManNAz under regular growth conditions, after 24 hours the media was changed and 30uM chloroquine was added. The following day cells were fixed and permeabilized. ManNaz was detected using Click-iT® reactions with an alkyne Alexa Fluor® 488 (Green). Cells were labeled with 0.5ug/mL anti-LC3B with a goat anti rabbit Alexa Fluor® 647 secondary (red) and 1ug/mL Hoechst 33342 (Blue). Cells were imaged on a Zeiss LSM 710 confocal microscope.
1% Agarose gel containing 16S and 23S ribosomal RNA (rRNA). SYBR® Green II RNA gel stain. Go ›
Bovine pulmonary artery endothelial cells (BPAEC). MitoTracker® Red CMXRos, SYTOX® Green nucleic acid stain, biotin-XX goat anti–mouse IgG antibody and Cascade Blue® NeutrAvidin biotin-binding protein. Go ›
REF-52 fibroblasts. Cyclic AMP Fluorosensor (FlCRhR) and fura-2 AM Go ›
BPAE cells fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit. Go ›
CD335 (NKp46) Antibody (63335182) in RE Go ›
CD223 (LAG-3) Antibody (56223942) in TM Go ›
Mouse Anti-Alpha Tubulin Monoclonal Antibody (Cat. No. A11126) Go ›
Muntjac fibroblast labeled with probes for actin and the nucleus. Go ›
BPAE cells fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit. Go ›
Cell proliferation detected with Click-iT® EdU and counter stained with anti-LC3B and a goat anti-rabbit Alexa Fluor® 647 secondary antibody. Go ›
Imaging autophagy in live HeLa cells with CellLight® reagents for mitochondria and lysosomes: Go ›
U-2 OS cells were transduced with Premo™ Autophagy sensor LC3B-RFP (red) and CellLight® Tubulin-GFP (green) Go ›