Simultaneous detection of three gene targets in a whole-mount Drosophila embryo by fluorescence in situ hybridization.

Pseudocolored green-fluorescent labeling represents a fluorescein-labeled cRNA probe detected using a rabbit anti-fluorescein/Oregon Green® primary antibody (Cat. No. A889) and an Alexa Fluor® 488 dye–labeled anti-rabbit secondary antibody (Cat. No. A11008). Pseudocolored yellow- and red-fluorescent labeling represents a biotinylated cRNA probe detected using HRP–streptavidin and Alexa Fluor® 568 tyramide (TSA Kit #24, Cat. No. T20934). Pseudocolored blue-fluorescent labeling represents a digoxigenin-labeled cRNA probe detected using a mouse anti-digoxigenin primary antibody in conjunction with an Alexa Fluor® 647 dye–labeled anti-mouse secondary antibody (Cat. No. A21235). The image was contributed by Ethan Bier and David Kosman, University of California, San Diego.

Pseudocolored green-fluorescent labeling represents a fluorescein-labeled cRNA probe detected using a rabbit anti-fluorescein/Oregon Green® primary antibody (Cat. No. A889) and an Alexa Fluor® 488 dye–labeled anti-rabbit secondary antibody (Cat. No. A11008). Pseudocolored yellow- and red-fluorescent labeling represents a biotinylated cRNA probe detected using HRP–streptavidin and Alexa Fluor® 568 tyramide (TSA Kit #24, Cat. No. T20934). Pseudocolored blue-fluorescent labeling represents a digoxigenin-labeled cRNA probe detected using a mouse anti-digoxigenin primary antibody in conjunction with an Alexa Fluor® 647 dye–labeled anti-mouse secondary antibody (Cat. No. A21235). The image was contributed by Ethan Bier and David Kosman, University of California, San Diego.

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