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Product Specifications
Analytical sensitivity
<78.1 pg/mL
Assay range
78.1-5000 pg/mL
Sample type/volume
Serum
100 µL
Supernatant
100 µL
Hands-on time
24 hr
Time-to-result
2 hr 50 min
Homogenous (no wash)
No
Instrument
Colorimetric Microplate Reader
Product size
5 x 96 Tests
Contents
Coating Antibody
Lyophilized Recombinant Standard
Detection Antibody
Streptavidin-HRP
Substrate Solution
Ready to use Stop Solution
0.16M Sulfuric Acid
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
Lyophilized Recombinant Standard
Detection Antibody
Streptavidin-HRP
Substrate Solution
Ready to use Stop Solution
0.16M Sulfuric Acid
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
IL-6
Species (tested)
Bovine
Assay kit format
Uncoated ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Bovine IL-6 Uncoated ELISA Kit contains pre-matched antibody pairs and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of bovine IL-6.
Principle of the method
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Target information
IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : IL6
Gene ID : (Bovine) 280826
Gene symbol : IL6
Protein Aliases : IL-6, interleukin 6 (interferon, beta 2), Interleukin-6
UniProt ID (Bovine) P26892
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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