Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

0.13 ng/mL

Assay range

0.13-25 ng/mL

Sample type/volume

Serum
50 µL
Plasma
50 µL
Supernatant
50 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

GM-CSF

Species (tested)

Dog

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

Canine GM-CSF quantitates canine GM-CSF in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant canine GM-CSF.

Target information

The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include interleukins 4, 5, and 13. The gene is involved in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : CSF2, GM-CSF

Gene ID : (Dog) 403923

Gene symbol : CSF2

Protein Aliases : Colony-stimulating factor, CSF, GM-CSF, Granulocyte-macrophage colony-stimulating factor

UniProt ID (Dog) P48749

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