Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

2.67 pg/mL

Assay range

62.5-2000 pg/mL

Sample type/volume

Cell Lysate
12.5 µL

Hands-on time

1 hr 20 min

Time-to-result

3 hr

Homogenous (no wash)

No

Interassay CV

6.3%

Intraassay CV

3.7%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Detection Antibody
Anti-Rabbit-HRP
Cell Lysis Buffer
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Dry ice

Storage

2-8°C

Protein name

ERK1/2

Target site-specificity

Phosphorylated Thr202, Phosphorylated Tyr204

Protein family

Other Proteins

Species (tested)

Human, Mouse, Rat

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

HRP

Label or dye

HRP

About This Kit

The ERK1/2 [pThr202/Tyr204] ELISA quantitates IL-1b in cell lysates. The assay will exclusively recognize both natural and recombinant ERK 1/2, when phosphorylated at Threonine 202 and Tyrosine 204.

Principle of the method

The phospho ERK1/2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Gene aliases : 9030612K14Rik, AA407128, AU018647, C78273, ERK, ERK-1, ERK-2, ERK1, ERK2, ERT1, ERT2, Esrk1, HS44KDAP, HUMKER1A, Mapk, MAPK1, MAPK2, MAPK3, MNK1, Mtap2k, p38, p40, p41, p41mapk, p42-MAPK, P42MAPK, p44, p44-ERK1, p44-MAPK, P44ERK1, P44MAPK, PRKM1, PRKM2, PRKM3

Gene ID : (Human) 5594, (Human) 5595, (Mouse) 26413, (Rat) 116590, (Rat) 50689, (Mouse) 26417

Gene symbol : MAPK1, MAPK3, Mapk1, Mapk1, Mapk3, Mapk3

Protein Aliases : ERK-1, ERK-2, ERT1, ERT2, Extracellular signal-regulated kinase 1, Extracellular signal-regulated kinase 2, extracellular signal-related kinase 1, extracellular-signal-regulated kinase 2, Insulin-stimulated MAP2 kinase, MAP kinase 1, MAP kinase 2, MAP kinase 3, MAP kinase isoform p42, MAP kinase isoform p44, MAPK 1, MAPK 2, MAPK 3, Microtubule-associated protein 2 kinase, mitogen activated protein kinase 1, mitogen-activated 3, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 2, Mitogen-activated protein kinase 3, MNK1, p42-MAPK, p44 MAP kinase, p44-ERK1, p44-MAPK, pp42/MAP kinase, protein tyrosine kinase ERK2

UniProt ID (Human) P28482, (Human) P27361, (Mouse) P63085, (Rat) P63086, (Rat) P21708, (Mouse) Q63844

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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