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Product Specifications
Analytical sensitivity
5.33 pg/mL
Assay range
19.66-12,000 pg/mL
Sample type/volume
Serum
50 µL
Plasma
50 µL
Fecal Extract
50 µL
Urine
12.5 µL
Supernatant
50 µL
Hands-on time
45 min
Time-to-result
2 hr 30 min
Homogenous (no wash)
No
Interassay CV
12%
Intraassay CV
10%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Anti-IgG coated 96 well plate
Standard
Assay Buffer Concentrate
Capture Antibody
HRP-conjugated Antigen
Wash Buffer Concentrate
TMB Substrate
Stop Solution
Plate Sealer
Standard
Assay Buffer Concentrate
Capture Antibody
HRP-conjugated Antigen
Wash Buffer Concentrate
TMB Substrate
Stop Solution
Plate Sealer
Shipping conditions
Dry ice
Storage
-20°C
Protein name
Estriol
Species (tested)
Human
Assay kit format
Competitive ELISA
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Estriol Competitive ELISA quantitates estriol in serum, plasma, urine, saliva, dried fecal extracts or or cell culture medium.|Principle of the method: The Estriol Competitive ELISA research-use-only kit is designed to quantitatively measure estriol independent of species. An estriol standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with anti-rabbit IgG antibodies. Anti-rabbit estriol antibodies and estriol-peroxidase conjugate is added to the wells. The estriol-peroxidase conjugate and any estriol in the sample will compete to bind to the anti-rabbit estriol antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound estriol-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of estriol present in the sample.|Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Estriol is the main estrogen in the blood and urine of pregnant females. Serum estriol determinations reflect the status of the fetus and are used to evaluate fetal distress and placental function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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